| 质粒名称: | pBiFC-bFOSDelataZipVC155 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22014 |
| 存储实验室: | Chang-Deng Hu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV-HA |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 3756 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | bFosDelataZIPVC155 |
| 物种来源: | R. norvegicus (rat); Aequorea Victoria |
| 插入大小: | 551 bp |
| 基因突变: | deletion of residues 179-193 in bFos(118-211) |
| 标签/融合蛋白: | HA（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | KpnI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | EBV-Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pBiFC-bFOSDelataZipVC155 was a gift from Chang-Deng Hu (Addgene plasmid # 22014) |
| 参考文献部分: | Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Jan . 40(1):61-6. 10.2144/000112036 PubMed 16454041 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBiFC-bFOSDelataZipVC155 4307 bp DNA SYN
DEFINITION pBiFC-bFOSDelataZipVC155
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4307
/organism="pBiFC-bFOSDelataZipVC155"
/mol_type="other DNA"
misc_feature 31..318
/label="CAG_enhancer"
promoter 36..528
/label="CMV_immearly_promoter"
misc_feature 485..505
/label="CMV_fwd_primer"
promoter 486..555
/label="CMV_promoter"
promoter 498..617
/label="CMV2_promoter"
misc_feature 529..548
/label="pCEP_fwd_primer"
misc_feature 531..555
/label="LNCX_primer"
misc_feature 631..657
/label="pCMV_primer"
misc_feature 672..768
/label="modSV40_late_16s_int"
misc_feature 672..702
/label="SV40_late_19s_int"
CDS 829..1434
/label="ORF frame 1"
misc_feature 832..858
/label="HA"
gene 1186..1431
/label="ECFP (468 - 713)"
/gene="ECFP (468 - 713)"
misc_feature 1368..1389
/label="EGFP_C_primer"
terminator complement(1456..1575)
/label="SV40_PA_terminator"
misc_feature complement(1470..1489)
/label="EBV_rev_primer"
promoter complement(1687..1705)
/label="M13_reverse_primer"
misc_feature complement(1704..1726)
/label="M13_pUC_rev_primer"
promoter complement(1740..1769)
/label="lac_promoter"
gene complement(2852..3712)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2852..3712)
/label="ORF frame 2"
promoter complement(3754..3782)
/label="AmpR_promoter"
misc_feature complement(3941..3963)
/label="pGEX_3_primer"
misc_feature complement(4162..4305)
/label="lacZ_a"
misc_feature 4276..4298
/label="M13_pUC_fwd_primer"
promoter 4291..4307
/label="M13_forward20_primer"
ORIGIN
1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC
601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG
661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG
721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA
781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GTACCCATAC
841 GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA TTCGGGGTCG TGCGCAGTCC
901 ATCGGTCGTC GCGGTAAAGT TGAACAACTG TCCCCGGAAG AGGAAGAGAA ACGTCGCATC
961 CGCCGTGAAC GTAACAAAAT GGCGGCAGCG AAATGCCGTA ACCGCCGTCG TGAACTGACC
1021 GACACCCTGC AGGCGGAAAC CGACCAGCTG GAAGACGAAA AATCCGCGGA GTTCATCCTG
1081 GCGGCACACC GTCCGGCGTG CAAAATCCCG AACGACCTGG GTGGTACCCG TCCGGCGTGC
1141 AAAATCCCGA ACGACCTGAA ACAGAAAGTC ATGAACCACG ACAAGCAGAA GAACGGCATC
1201 AAGGCCAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC
1261 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
1321 AGCTACCAGT CCAAACTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
1381 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTAAGCGGCC
1441 GCGGGGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC
1501 AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
1561 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
1621 GGGAGGTGTG GGAGGTTTTT TCGGATCCTC TAGAGTCGAT CTGCAGGCAT GCTAGCTTGG
1681 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
1741 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
1801 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
1861 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
1921 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
1981 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
2041 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
2101 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
2161 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
2221 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
2281 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
2341 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
2401 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
2461 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
2521 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
2581 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
2641 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
2701 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
2761 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
2821 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
2881 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
2941 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
3001 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
3061 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
3121 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
3181 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
3241 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
3301 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
3361 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
3421 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
3481 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
3541 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
3601 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
3661 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
3721 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
3781 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
3841 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
3901 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC
3961 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG
4021 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG
4081 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC
4141 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
4201 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
4261 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGT
//
