| 质粒名称: | LZRS-HOTAIR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26110 |
| 存储实验室: | Howard Chang |
|
载体骨架基本信息 |
|
| 载体名称: | LZRS |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 11100 bp |
| 完整质粒大小: | 13246 bp |
| 载体类型: | Retroviral |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HOTAIR |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2146 bp |
| 克隆方法: | Gateway Cloning |
| 5' 克隆位点: | attR(破坏) |
| 3' 克隆位点: | attR(破坏) |
| 5' 测序引物 | TGGATACACGCCGCCCACGTG |
| 3' 测序引物 | ATCGTCGACCACTGTGCTGG |
|
文献引用方法 |
|
| 材料和方法部分: | LZRS-HOTAIR was a gift from Howard Chang (爱迪基因 plasmid # 26110) |
| 参考文献部分: | Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, Hung T, Argani P, Rinn JL, Wang Y, Brzoska P, Kong B, Li R, West RB, van de Vijver MJ, Sukumar S, Chang HY. Nature. 2010 Apr 15. 464(7291):1071-6. 10.1038/nature08975 PubMed 20393566 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS LZRS-HOTAIR 13046 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS LZRS-HOTAIR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13046)
AUTHORS Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC,
Hung T, Argani P, Rinn JL, Wang Y, Brzoska P, Kong B, Li R, West RB,
van de Vijver MJ, Sukumar S, Chang HY
TITLE Long non-coding RNA HOTAIR reprograms chromatin state to promote
cancer metastasis.
JOURNAL Nature. 2010 Apr 15. 464(7291):1071-6.
PUBMED 20393566
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|LZRS-HOTAIR|
FEATURES Location/Qualifiers
source 1..13046
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 52..521
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 586..943
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1008..1424
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1354..1376
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1392..1408
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 1434..1808
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 1746..1762
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
LTR 4255..4724
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(4854..4871)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5025..5613)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5105..5124)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
polyA_signal 5736..5870
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5760..5779)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5814..5833
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(6100..6699)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6183..6203)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 6680..6699
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
promoter complement(6782..7281)
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(6830..6849)
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
primer_bind 7223..7246
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
rep_origin 9313..11102
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
CDS complement(11596..12456)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12219..12238
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12457..12561)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cataactgag aatagaaaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
61 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
121 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
181 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
241 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
301 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
361 cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
421 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
481 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
541 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
601 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
661 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
721 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
781 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctta gaggagggat
841 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
901 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
961 gtctctgtct gactgtgttt ctgtatttgt ctgaaaattt gggcccgggc cagactgtta
1021 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc
1081 agtcggtagt tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct
1141 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga
1201 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct
1261 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc
1321 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc
1381 ctcgatcctc cctttatcca gccctcactc cttctctagg cgcccccata tggccatatg
1441 agatcttata tggggcaccc ccgccccttg taaacttccc tgaccctgac ttgacaagag
1501 ttactaacag cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag
1561 tctggagacc tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc
1621 cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc
1681 gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa gtagacggca
1741 tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct
1801 ctagactgcc ggatccagac gctgcgtttg ctggctttga tgaaagactc gcctgtgctc
1861 tggagcttga tccgaaagct tccacagtga ggactgctcc gtgggggtaa gagagcacca
1921 ggcactgagg cctgggagtt ccacagacca acacccctgc tcctggcggc tcccacccgg
1981 ggcttagacc ctcaggtccc taatatcccg gaggtgctct caatcagaaa ggtcctgctc
2041 cgcttcgcag tggaatggaa cggatttaga agcctgcagt aggggagtgg ggagtggaga
2101 gagggagccc agagttacag acggcggcga gaggaaggag gggcgtcttt atttttttaa
2161 ggccccaaag agtctgatgt ttacaagacc agaaatgcca cggccgcgtc ctggcagaga
2221 aaaggctgaa atggaggacc ggcgccttcc ttataagtat gcacattggc gagagaatta
2281 agtgctgcaa cctaaaccag caattacacc caagctcgtt ggggcctaag ccagtaccga
2341 cctggtagaa aaagcaacca cgaagctaga gagagagcca gaggagggaa gagagcgcca
2401 gacgaaggtg aaagcgaacc acgcagagaa atgcaggcaa gggagcaagg cggcagttcc
2461 cggaacaaac gtggcagagg gcaagacggg cactcacaga cagaggttta tgtattttta
2521 ttttttaaaa tctgatttgg tgttccatga ggaaaaggga aaatctaggg aacgggagta
2581 cagagagaat aatccgggtc ctagctcgcc acatgaacgc ccagagaacg ctggaaaaac
2641 ctgagcgggt gccggggcag cacccggctc gggtcagcca ctgccccaca ccgggcccac
2701 caagccccgc ccctcgcggc caccggggct tccttgctct tcttatcatc tccatcttta
2761 tgatgaggct tgttaacaag accagagagc tggccaagca cctctatctc agccgcgccc
2821 gctcagccga gcagcggtcg gtggggggac tgggaggcgc taattaattg attcctttgg
2881 actgtaaaat atggcggcgt ctacacggaa cccatggact cataaacaat atatctgttg
2941 ggcgtgagtg cactgtctct caaataattt ttccataggc aaatgtcaga gggttctgga
3001 tttttagttg ctaaggaaag atccaaatgg gaccaatttt aggaggccca aacagagtcc
3061 gttcagtgtc agaaaatgct tccccaaagg gttggcagtg tgttttgttg gaaaaaagct
3121 tgggttatag gaaagccttt ccctgctact tgtgtagacc cagcccaatt taagaattac
3181 aaggaagcga aggggttgtg taggccggaa gcctctctgt cccggctgga tgcaggggac
3241 ttgagctgct ccggaatttg agaggaacat agaagcaaag gtccagcctt tgcttcgtgc
3301 tgattcctag acttaagatt caaaaacaaa tttttaaaag tgaaaccagc cctagccttt
3361 ggaagctctt gaaggttcag cacccaccca ggaatccacc tgcctgttac acgcctctcc
3421 aagacacagt ggcaccgctt ttctaactgg cagcacagag caactctata atatgcttat
3481 attaggtcta gaagaatgca tcttgagaca catgggtaac ctaattatat aatgcttgtt
3541 ccatacagga gtgattatgc agtgggaccc tgctgcaaac gggactttgc actctaaata
3601 taggccccag cttgggacaa aagttgcagt agaaaaatag acataggaga acacttaaat
3661 aagtgatgca tgtagacaca gaaggggtat ttaaaagaca gaaataatag aagtacagaa
3721 gaacagaaaa aaaatcagca gatggagatt accattccca atgcctgaac ttcctcctgc
3781 tattaagatt gctagagaat tgtgtcttaa acagttcatg aacccagaag aacgcaattt
3841 caatgtattt agtacacaca cagtatgtat ataaacacaa ctcacagaat atattttcca
3901 tacattgggt aggtatgcac tttgtgtata tataataatg tattttccat gcagttttaa
3961 aatgtagata tattaatatc tggatgcatt ttcaaaaaaa aaacctatag tggtcgtatt
4021 aattcggatc cgcgaagggc gaattctgca gatatccatc acactggcgg ccgccagcac
4081 agtggtcgac gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
4141 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
4201 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat
4261 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
4321 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
4381 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
4441 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
4501 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
4561 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
4621 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
4681 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca
4741 aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga
4801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
4861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
4921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
4981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
5041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
5101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
5161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
5221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
5281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
5341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
5401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
5461 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
5521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
5581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
5641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
5701 aggatcttca cctagatcct tttgcggccg aattagatcc agacatgata agatacattg
5761 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
5821 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
5881 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
5941 aaaacctcta caaatgtggt atggctgatt atgatcctct agagtcggtg ggcctcgggg
6001 gcgggtgcgg ggtcggcggg gccgccccgg gtggcttcgg tcggagccat ggggtcgtgc
6061 gctcctttcg gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt
6121 catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag
6181 ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc
6241 gcgctcggcc gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg
6301 gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc
6361 caggaggcct tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg
6421 cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc
6481 caccgcggcg ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag
6541 gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccgggtcga cggtgtggcg
6601 cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggcccggg ggacgtcgtc
6661 gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gaaggtcgtc tccttgtgag
6721 gggtcagggg cgtgggtcag gggatggtgg cggcaccggt cgtggcggcc gacctgcagg
6781 tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg
6841 tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc
6901 tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa
6961 gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca
7021 tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct
7081 gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac
7141 caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc
7201 cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg
7261 ggaaaagcgc ctcccctacc cggtagaatt aattctcatg tttgacagct tatcatcgat
7321 aagctgatcc tcacaggccg cacccagctt ttcttccgtt gccccagtag catctctgtc
7381 tggtgacctt gaagaggaag aggaggggtc ccgagaatcc ccatccctac cgtccagcaa
7441 aaagggggac gaggaatttg aggcctggct tgaggctcag gacgcaaatc ttgaggatgt
7501 tcagcgggag ttttccgggc tgcgagtaat tggtgatgag gacgaggatg gttcggagga
7561 tggggaattt tcagacctgg atctgtctga cagcgaccat gaaggggatg agggtggggg
7621 ggctgttgga gggggcagga gtctgcactc cctgtattca ctgagcgtcg tctaataaag
7681 atgtctattg atctctttta gtgtgaatca tgtctgacga ggggccaggt acaggacctg
7741 gaaatggcct aggagagaag ggagacacat ctggaccaga aggctccggc ggcagtggac
7801 ctcaaagaag agggggtgat aaccatggac gaggacgggg aagaggacga ggacgaggag
7861 gcggaagacc aggagccccg ggcggctcag gatcagggcc aagacataga gatggtgtcc
7921 ggagacccca aaaacgtcca agttgcattg gctgcaaagg gacccacggt ggaacaggag
7981 caggagcagg agcgggaggg gcaggagcag gaggggcagg agcaggagga ggggcaggag
8041 caggaggggc aggagcagga ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc
8101 gaggaggtag tggaggccgg ggtcgaggag gtagtggagg ccgccggggt agaggacgtg
8161 aaagagccag ggggggaagt cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa
8221 agaggcccag gagtcccagt agtcagtcat catcatccgg gtctccaccg cgcaggcccc
8281 ctccaggtag aaggccattt ttccaccctg taggggaagc cgattatttt gaataccacc
8341 aagaaggtgg cccagatggt gagcctgacg tgcccccggg agcgatagag cagggccccg
8401 cagatgaccc aggagaaggc ccaagcactg gaccccgggg tcagggtgat ggaggcaggc
8461 gcaaaaaagg agggtggttt ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg
8521 agaacattgc agaaggttta agagctctcc tggctaggag tcacgtagaa aggactaccg
8581 acgaaggaac ttgggtcgcc ggtgtgttcg tatatggagg tagtaagacc tccctttaca
8641 acctaaggcg aggaactgcc cttgctattc cacaatgtcg tcttacacca ttgagtcgtc
8701 tcccctttgg aatggcccct ggacccggcc cacaacctgg cccgctaagg gagtccattg
8761 tctgttattt catggtcttt ttacaaactc atatatttgc tgaggttttg aaggatgcga
8821 ttaaggacct tgttatgaca aagcccgctc ctacctgcaa tatcagggtg actgtgtgca
8881 gctttgacga tggagtagat ttgcctccct ggtttccacc tatggtggaa ggggctgccg
8941 cggagggtga tgacggagat gacggagatg aaggaggtga tggagatgag ggtgaggaag
9001 ggcaggagtg atgtaacttg ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc
9061 cccgcactaa agaataaatc cccagtagac atcatgcgtg ctgttggtgt atttctggcc
9121 atctgtcttg tcaccatttt cgtcctccca acatggggca attgggcata cccatgttgt
9181 cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac
9241 ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat
9301 gggagcaacc agcaggaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg
9361 gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg
9421 catgaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
9481 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
9541 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
9601 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta cccagatata
9661 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca gatataaatt
9721 aggatagcat atactaccct aatctctatt aggatagcat atgctacccg gatacagatt
9781 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
9841 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
9901 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
9961 aggatagcat atgctatcca gatatttggg tagtatatgc tacccatggc aacattagcc
10021 caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag
10081 gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc
10141 ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt
10201 gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc
10261 aaaaccgcag ggcggcgtgt gggggctgac gcgtgccccc actccacaat ttcaaaaaaa
10321 agagtggcca cttgtctttg tttatgggcc ccattggcgt ggagccccgt ttaattttcg
10381 ggggtgttag agacaaccag tggagtccgc tgctgtcggc gtccactctc tttccccttg
10441 ttacaaatag agtgtaacaa catggttcac ctgtcttggt ccctgcctgg gacacatctt
10501 aataacccca gtatcatatt gcactaggat tatgtgttgc ccatagccat aaattcgtgt
10561 gagatggaca tccagtcttt acggcttgtc cccaccccat ggatttctat tgttaaagat
10621 attcagaatg tttcattcct acactagtat ttattgccca aggggtttgt gagggttata
10681 ttggtgtcat agcacaatgc caccactgaa ccccccgtcc aaattttatt ctgggggcgt
10741 cacctgaaac cttgttttcg agcacctcac atacacctta ctgttcacaa ctcagcagtt
10801 attctattag ctaaacgaag gagaatgaag aagcaggcga agattcagga gagttcactg
10861 cccgctcctt gatcttcagc cactgccctt gtgactaaaa tggttcacta ccctcgtgga
10921 atcctgaccc catgtaaata aaaccgtgac agctcatggg gtgggagata tcgctgttcc
10981 ttaggaccct tttactaacc ctaattcgat agcatatgct tcccgttggg taacatatgc
11041 tattgaatta gggttagtct ggatagtata tactactacc cgggaagcat atgctacccg
11101 tttagggtta acaagggggc cttataaaca ctattgctaa tgccctcttg agggtccgct
11161 tatcggtagc tacacaggcc cctctgattg acgttggtgt agcctcccgt agtcttcctg
11221 ggcccctggg aggtacatgt cccccagcat tggtgtaaga gcttcagcca agagttacac
11281 ataaaggcaa tgttgtgttg cagtccacag actgcaaagt ctgctccagg atgaaagcca
11341 ctcagtgttg gcaaatgtgc acatccattt ataaggatgt caactacagt cagagaaccc
11401 ctttgtgttt ggtccccccc cgtgtcacat gtggaacagg gcccagttgg caagttgtac
11461 caaccaactg aagggattac atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc
11521 aggtttagtt cgtccggggc ggggatctgg ccgcaaatca atctaaagta tatatgagta
11581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
11641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
11701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
11761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
11821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
11881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
11941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
12001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
12061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
12121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
12181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
12241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
12301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
12361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
12421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
12481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
12541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccaccagctt tgctcttagg
12601 agtttcctaa tacatcccaa actcaaatat ataaagcatt tgacttgttc tatgccctag
12661 ggggcggggg gaagctaagc cagctttttt taacatttaa aatgttaatt ccattttaaa
12721 tgcacagatg tttttatttc ataagggttt caatgtgcat gaatgctgca atattcctgt
12781 taccaaagct agtataaata aaaatagata aacgtggaaa ttacttagag tttctgtcat
12841 taacgtttcc ttcctcagtt gacaacataa atgcgctgct gagaagccag tttgcatctg
12901 tcaggatcaa tttcccatta tgccagtcat attaattact agtcaattag ttgattttta
12961 tttttgacat atacatgtga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
13021 gccattttgc aaggcatgga aaaata
//
