| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-TetOne |
| 质粒类型: | 慢病毒载体；四环素调控系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | TRE3GS |
| 载体大小: | 8279 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 慢病毒载体pLVX-TetOne是集调控与应答于一体的四环素诱导载体。 |
| 产品目录号: | 631846 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-TetOne 8279 bp ds-DNA circular DNA 11-JUL-2013
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT 631844, 631845, 631846
FEATURES Location/Qualifiers
LTR 1..635
/label="5' LTR"
misc_feature 681..806
/label="packaging signal"
misc_feature 1303..1536
/label="RRE"
misc_feature 2028..2144
/label="cPPT/CTS"
polyA_signal 2187..2321
/label="SV40 polyA signal"
misc_feature complement(2496..2527)
/label="MCS"
misc_feature complement(2528..2892)
/label="TRE3GS promoter"
promoter 2912..3422
/label="hPGK promoter"
CDS 3441..4187
/label="Tet-On 3G"
misc_feature 4201..4789
/label="WPRE"
LTR 4997..5630
/label="3' LTR"
rep_origin complement(6161..6746)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6917..7777)
/label="AmpR"
promoter complement(7778..7882)
/label="AmpR promoter"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc cgccggcacc ggtgtatacg
2521 ggaattcttt acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg
2581 gaattgaagt ttactcaaaa aatcagcact cttttatagg cgccctggtt tacataagca
2641 aagcttatac gttctctatc actgataggg agtaaactgg atatacgttc tctatcactg
2701 atagggagta aactgtagat acgttctcta tcactgatag ggagtaaact ggtcatacgt
2761 tctctatcac tgatagggag taaactcctt atacgttctc tatcactgat agggagtaaa
2821 gtctgcatac gttctctatc actgataggg agtaaactct tcatacgttc tctatcactg
2881 atagggagta aactcgaggt gataattcca cggggttggg gttgcgcctt ttccaaggca
2941 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
3001 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
3061 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
3121 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
3181 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
3241 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
3301 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
3361 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
3421 agggggatca tcgaattacc atgtctagac tggacaagag caaagtcata aactctgctc
3481 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
3541 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
3601 ccctgccaat cgagatgctg gacaggcatc atacccactc ctgccccctg gaaggcgagt
3661 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
3721 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
3781 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
3841 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
3901 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg aaacaagcaa
3961 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
4021 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg
4081 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc
4141 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaaacg cgtctggaac
4201 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
4261 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
4321 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
4381 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
4441 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
4501 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
4561 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
4621 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
4681 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
4741 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
4801 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
4861 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt tccagtcaca
4921 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
4981 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
5041 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
5101 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
5161 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
5221 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
5281 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
5341 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
5401 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
5461 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
5521 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
5581 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
5641 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
5701 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt
5761 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
5821 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
5881 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
5941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
6001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
6061 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
6121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
6181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
6241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
6301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
6361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
6421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
6481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
6541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
6601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
6661 gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga
6721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
6781 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
6841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
6901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
6961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
7021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
7081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
7141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
7201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
7261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
7321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
7381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
7441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
7501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
7561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
7621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
7681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
7741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
7801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
7861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
7921 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
7981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
8041 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
8101 cttcccaccc cataccctat taccactgcc aattacctag tggtttcatt tactctaaac
8161 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
8221 aaacttagta gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
