| 质粒名称: | pCMV-MCP-mCherry |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0003060 |
| 产品用途: | Mammalian expression of MCP-fused mCherry for MCP-MS2 tethering assay |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MCP-mCherry |
| 物种来源: |
-
|
| 密码子优化: | - |
|
文献引用方法 |
|
| 参考文献部分: |
Alphaviral non-structural protein-host RBP co-condensation as a mechanism to sustain virus replication
Molecular Cell, Volume 86, Issue 9, 2026
Yi Liu, Peiguo Yang
|
pCMV-MCP-mCherry
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCMV-MCP-mCherry载体质粒基本信息
pCMV-MCP-mCherry质粒图谱载体图谱和pCMV-MCP-mCherry载体序列质粒序列多克隆位点信息
pCMV-MCP-mCherry质粒载体简介
pCMV-MCP-mCherry质粒序列载体序列
LOCUS pCMV-MCP-mCherry 5052 bp DNA circular SYN 12-AUG-2026
DEFINITION Localization: C1 Cloning Vector, Excitation: 488, Emission: 507.
ACCESSION .
VERSION .
KEYWORDS 3060-pCMV-MCP-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5052)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 5052)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 7, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5052
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 142..445
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 446..649
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 600..620
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind complement(672..708)
/label=引物 6
primer_bind 679..717
/label=引物 1
regulatory 688..693
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 694..1080
/codon_start=1
/label=MCP
/translation="MLAVKMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRS
QAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGL
LKDGNPIPSAIAANSGIYADPPVAT"
primer_bind 1087..1104
/label=引物 1
CDS 1102..1809
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(1787..1827)
/label=引物 2
primer_bind 1795..1836
/label=引物 3
polyA_signal 1921..2042
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1958..1977)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2012..2031
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2049..2504)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2186..2207)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2398..2417
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2531..2635
/gene="bla"
/label=AmpR promoter
promoter 2637..2994
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2660..2680)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2845..2980
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2907..2926
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2930..2941)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3029..3823
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3083..3102)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3693..3712
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4011..4030)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4055..4102
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4431..5019
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4920..4939
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
61 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
121 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
181 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
241 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
301 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
361 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
421 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
481 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
541 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
601 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
661 ccgtcagatc cgctagcgct accggtcgcc accatgctag ccgttaaaat ggcttctaac
721 tttactcagt tcgttctcgt cgacaatggc ggaactggcg acgtgactgt cgccccaagc
781 aacttcgcta acgggatcgc tgaatggatc agctctaact cgcgttcaca ggcttacaaa
841 gtaacctgta gcgttcgtca gagctctgcg cagaatcgca aatacaccat caaagtcgag
901 gtgcctaaag gcgcctggcg ttcgtactta aatatggaac taaccattcc aattttcgcc
961 acgaattccg actgcgagct tattgttaag gcaatgcaag gtctcctaaa agatggaaac
1021 ccgattccct cagcaatcgc agcaaactcc ggcatctacg cggatccacc ggtcgccacc
1081 ctagcgctac cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc
1141 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
1201 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
1261 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
1321 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
1381 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
1441 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
1501 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
1561 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
1621 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
1681 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
1741 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
1801 ctgtacaagt aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
1861 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
1921 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
1981 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
2041 taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
2101 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
2161 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
2221 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
2281 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
2341 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
2401 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
2461 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
2521 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
2581 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
2641 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
2701 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
2761 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
2821 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
2881 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
2941 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
3001 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
3061 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
3121 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
3181 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
3241 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
3301 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
3361 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
3421 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
3481 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
3541 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
3601 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
3661 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
3721 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
3781 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
3841 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
3901 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
3961 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
4021 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
4081 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
4141 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
4201 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
4261 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
4321 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
4381 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
4441 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
4501 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
4561 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
4621 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
4681 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
4741 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
4801 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
4861 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
4921 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
4981 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
5041 ttacggttcc tg
//
