| 质粒名称: | pQL162 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002532 |
| 产品用途: | Synthetic PhSurv-driven mCherry expression vector (PhSurv-mCherry-pA) |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | Synthetic |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | mCherry |
| 物种来源: | Discosoma |
| 密码子优化: | 未优化 |
pQL162
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pQL162载体质粒基本信息
pQL162质粒图谱载体图谱和pQL162载体序列质粒序列多克隆位点信息
pQL162质粒载体简介
pQL162质粒序列载体序列
LOCUS pQL162 5661 bp DNA circular SYN 08-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 5661)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5661
/mol_type="other DNA"
/organism="recombinant plasmid"
primer_bind 209..229
/label=QL426-F
primer_bind 213..257
/label=QL414-F
misc_feature 234..463
/label=-230
misc_feature 234..463
/label=-1430
primer_bind complement(298..312)
/label=QL138-R
primer_bind 459..519
/label=QL 431-F
primer_bind 460..519
/label=QL 432-F
misc_feature 464..493
/label=5UTR
misc_feature 464..493
/label=+30
primer_bind complement(473..514)
/label=QL425-R
primer_bind complement(480..499)
/label=QL571 R
primer_bind 499..518
/label=QL 429-F
CDS 500
/codon_start=1
/product="start codon"
/label=ATG
/translation=""
CDS 503..1207
/codon_start=1
/label=mCherry
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(1187..1232)
/label=QL138-R
primer_bind complement(1190..1211)
/label=QL144-R
primer_bind complement(1191..1243)
/label=QL 430-R
terminator 1258..1485
/label=bGH PA
primer_bind complement(1495..1517)
/label=QL215 R
misc_feature 2153..2230
/label=SV40 ORI
CDS 2372..3163
/label=Kan/neoR
primer_bind complement(3503..3523)
/label=M13-rev
misc_binding complement(3529..3551)
/label=LacO
promoter complement(3556..3585)
/label=lac
CDS complement(4668..5327)
/label=AmpR
promoter complement(5567..5595)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgcgtgag
241 ccactgcacc cggcctgcac gcgttctttg aaagcagtcg agggggcgct aggtgtgggc
301 agggacgagc tggcgcggcg tcgctgggtg caccgcgacc acgggcagag ccacgcggcg
361 ggaggactac aactcccggc acaccccgcg ccgccccgcc tctactccca gaaggccgcg
421 gggggtggac cgcctaagag ggcgtgcgct cccgacatgc cccgcggcgc gccattaacc
481 gccagatttg aatgaattca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
541 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
601 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
661 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
721 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
781 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
841 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
901 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
961 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
1021 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
1081 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
1141 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
1201 gtacaagtaa gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
1261 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
1321 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
1381 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
1441 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
1501 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
1561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
1621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
1681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
1741 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
1801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
1861 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
1921 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
1981 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
2041 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2101 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
2161 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
2221 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
2281 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
2341 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
2401 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
2461 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
2521 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
2581 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
2641 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
2701 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
2761 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
2821 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
2881 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
2941 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
3001 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
3061 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
3121 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
3181 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
3241 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
3301 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
3361 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
3421 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
3481 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
3541 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
3601 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
3661 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
3721 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
3781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
3841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
3901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
3961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
4021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
4081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
4141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
4201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
4261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
4321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
4381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
4441 agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
4501 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4561 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4621 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
4681 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4741 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
4801 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4861 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4921 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
4981 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
5041 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
5101 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
5161 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
5221 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
5281 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
5341 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
5401 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
5461 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
5521 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5581 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5641 cgaaaagtgc cacctgacgt c
//
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