| 质粒名称: | ABE8.20-m |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 136300 |
| 存储实验室: | Nicole Gaudelli |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322, beta-lactamase maintenance, CMV promoter |
| 载体出品公司: | -- |
| 空载体大小: | 3400 bp |
| 完整质粒大小: | 8166 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ABE8.20-m |
| 物种来源: | -- |
| 插入大小: | 4800 bp |
| 基因突变: | D10A in S. pyogenes Cas9, TadA mutations described in manuscript |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | C-terminal BPNLS（插入件上的C端子） |
| 克隆方法: | Ligation Independent克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7 |
| 3’测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | ABE8.20-m was a gift from Nicole Gaudelli (Addgene plasmid # 136300 ; http://n2t.net/addgene:136300 ; RRID:Addgene_136300) |
| 参考文献部分: | Directed evolution of adenine base editors with increased activity and therapeutic application. Gaudelli NM, Lam DK, Rees HA, Sola-Esteves NM, Barrera LA, Born DA, Edwards A, Gehrke JM, Lee SJ, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. 10.1038/s41587-020-0491-6 PubMed 32284586 |
买家导航
ABE8.20-m载体 ABE8.20m质粒基本信息
ABE8.20-m质粒图谱 ABE8.20m载体图谱和ABE8.20-m载体序列 ABE8.20m质粒序列多克隆位点信息
ABE8.20-m质粒 ABE8.20m载体简介
ABE8.20-m质粒序列 ABE8.20m载体序列
LOCUS ABE8.20-m 8166 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION expresses ABE8.20-m in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS ABE8.20-m
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8166)
AUTHORS Gaudelli NM, Lam DK, Rees HA, Sola-Esteves NM, Barrera LA, Born DA,
Edwards A, Gehrke JM, Lee SJ, Liquori AJ, Murray R, Packer MS,
Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G
TITLE Directed evolution of adenine base editors with increased activity
and therapeutic application.
JOURNAL Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi:
10.1038/s41587-020-0491-6.
PUBMED 32284586
REFERENCE 2 (bases 1 to 8166)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8166
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 103..482
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 483..686
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 637..657
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 728..747
/label=T7
/note="T7 promoter, forward primer"
promoter 728..746
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 763..1260
/codon_start=1
/product="DNA adenosine deaminase evolved from the E. coli
tRNA adenosine deaminase TadA (Gaudelli et al., 2017)"
/label=TadA*(7.10)
/translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
RAIGLHDPTAHAEIMALRQGGLVMQNYRLYDATLYSTFEPCVMCAGAMIHSRIGRVVFG
VRNAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLCRFFRMPRRVFNAQKKAQS
STD"
CDS 1357..5457
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5503..5523
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5532..5549
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5572..5589)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5578..5802
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5873..5889)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5873..5889)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5886..5908)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5897..5913
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5921..5951)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5966..5987
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6104..6121)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6275..6863)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6355..6374)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7034..7894)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7657..7676
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7895..7999)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8078..8097)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
61 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
121 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
181 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
241 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
301 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
361 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
421 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
481 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
541 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
601 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
661 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagagatccg
721 cggccgctaa tacgactcac tatagggaga gccgccacca tgtccgaagt cgagttttcc
781 catgagtact ggatgagaca cgcattgact ctcgcaaaga gggctcgaga tgaacgcgag
841 gtgcccgtgg gggcagtact cgtgctcaac aatcgcgtaa tcggcgaagg ttggaatagg
901 gcaatcggac tccacgaccc cactgcacat gcggaaatca tggcccttcg acagggaggg
961 cttgtgatgc agaattatcg actttatgat gcgacgctgt actcgacgtt tgaaccttgc
1021 gtaatgtgcg cgggagctat gattcactcc cgcattggac gagttgtatt cggtgttcgc
1081 aacgccaaga cgggtgccgc aggttcactg atggacgtgc tgcatcatcc aggcatgaac
1141 caccgggtag aaatcacaga aggcatattg gcggacgaat gtgcggcgct gttgtgtcgt
1201 ttttttcgca tgcccaggcg ggtctttaac gcccagaaaa aagcacaatc ctctactgac
1261 tctggtggtt cttctggtgg ttctagcggc agcgagactc ccgggacctc agagtccgcc
1321 acacccgaaa gttctggtgg ttcttctggt ggttctgaca agaagtacag catcggcctg
1381 gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
1441 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
1501 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
1561 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
1621 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
1681 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
1741 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
1801 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
1861 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
1921 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
1981 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
2041 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
2101 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
2161 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
2221 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
2281 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
2341 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
2401 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
2461 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
2521 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
2581 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
2641 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
2701 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
2761 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
2821 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
2881 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
2941 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
3001 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
3061 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
3121 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
3181 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
3241 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
3301 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
3361 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
3421 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
3481 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
3541 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
3601 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
3661 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
3721 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
3781 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
3841 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
3901 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
3961 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
4021 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
4081 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
4141 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
4201 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
4261 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
4321 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
4381 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
4441 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
4501 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
4561 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
4621 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
4681 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
4741 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
4801 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
4861 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
4921 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
4981 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
5041 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
5101 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
5161 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
5221 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
5281 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
5341 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
5401 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgacgag
5461 ggagctgata agcgcaccgc cgatggttcc gagttcgaaa gccccaagaa gaagaggaaa
5521 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
5581 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
5641 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
5701 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
5761 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
5821 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
5881 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
5941 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
6001 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
6061 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
6121 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
6181 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
6241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
6301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
6361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
6421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
6481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
6541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
6601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
6661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
6721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
6781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
6841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
6901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
6961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
7021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
7081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
7141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
7201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
7261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
7321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
7381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
7441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
7501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
7561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
7621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
7681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
7741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
7801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
7861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
7921 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
7981 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
8041 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat
8101 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
8161 aaattt
//
