| 质粒名称: | GFP-TAF1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 65395 |
| 存储实验室: | Kyle Miller |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA6.2/N-EmGFP-DEST |
| 载体出品公司: | invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5847 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 杀稻瘟菌素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TAF1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 5616 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: |
EmGFP（主干上的N终端）
V5（主干上的C终端）
|
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | EGFP_C_primer |
| 3' 测序引物 | TK polyA reverse primer |
|
文献引用方法 |
|
| 材料和方法部分: | GFP-TAF1 was a gift from Kyle Miller (Addgene plasmid # 65395 ; http://n2t.net/addgene:65395 ; RRID:Addgene_65395) |
| 参考文献部分: | Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM. Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114. 10.1101/gad.252189.114 PubMed 25593309 |
买家导航
GFP-TAF1载体 GFPTAF1质粒基本信息
GFP-TAF1质粒图谱 GFPTAF1载体图谱和GFP-TAF1载体序列 GFPTAF1质粒序列多克隆位点信息
GFP-TAF1质粒 GFPTAF1载体简介
GFP-TAF1质粒序列 GFPTAF1载体序列
LOCUS GFP-TAF1 11569 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION GFP-tagged BRD protein, which can be used to be expressed in
mammaiian cells..
ACCESSION .
VERSION .
KEYWORDS GFP-TAF1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11569)
AUTHORS Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
TITLE Screen identifies bromodomain protein ZMYND8 in chromatin
recognition of transcription-associated DNA damage that promotes
homologous recombination.
JOURNAL Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
PUBMED 25593309
REFERENCE 2 (bases 1 to 11569)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11569
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(11..28)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(262..281)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1564..1583
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
primer_bind complement(1981..2000)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 2172..2551
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2552..2755
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2706..2726
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2752..2776
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2880..3596
/codon_start=1
/product="Emerald GFP"
/label=EmGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2925..2946)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3186..3205)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3533..3554
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind 3612..3636
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(9331..9355)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 9363..9404
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
primer_bind complement(9444..9463)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 9488..9536
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 9738..10166
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9825..9844)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 10035..10056
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(10175..10195)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 10180..10509
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10360..10495
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10422..10441
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 10557..10604
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10623..11021
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 11179..11300
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11216..11235)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 11270..11289
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(11349..11365)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11349..11365)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11362..11384)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11373..11389
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11397..11427)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11442..11463
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
2761 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
2821 ccgggaccga tccagcctcc ggactctaga ggatccctac cggtgatatc ctcgagacca
2881 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
2941 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
3001 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
3061 tcgtgaccac cttcacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
3121 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
3181 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
3241 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
3301 agctggagta caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg
3361 gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg
3421 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
3481 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
3541 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggct
3601 cgagcccatc aacaagtttg tacaaaaaag caggcttcga aggagataga accaattctc
3661 taaggaaata cttaaccatg gtcgacatgg gacccggctg cgatttgctg ctgcggacag
3721 cagctaccat cactgctgcc gccatcatgt cagacacgga cagcgacgaa gattccgctg
3781 gaggcggccc attttcttta gcgggtttcc ttttcggcaa catcaatgga gccgggcagc
3841 tggaggggga aagcgtcttg gatgatgaat gtaagaagca cttggcaggc ttgggggctt
3901 tggggctggg cagcctgatc actgaactca cggcaaatga agaattgacc gggactgacg
3961 gtgccttggt aaatgatgaa gggtgggtta ggagtacaga agatgctgtg gactattcag
4021 acatcaatga ggtggcagaa gatgaaagcc gaagatacca gcagacgatg gggagcttgc
4081 agcccctttg ccactcagat tatgatgaag atgactatga tgctgattgt gaagacattg
4141 attgcaagtt gatgcctcct ccacctccac ccccgggacc aatgaagaag gataaggacc
4201 aggattctat tactggtgag aaagtggact tcagtagttc ctctgactca gaatctgaga
4261 tgggacctca ggaagcaaca caggcagaat ctgaagatgg aaagctgacc cttccattgg
4321 ctgggattat gcagcatgat gccaccaagc tgttgccaag tgtcacagaa ctttttccag
4381 aatttcgacc tggaaaggtg ttacgttttc tacgtctttt tggaccaggg aagaatgtcc
4441 catctgtttg gcggagtgct cggagaaaga ggaagaagaa gcaccgtgag ctgatacagg
4501 aagagcagat ccaggaggtg gagtgctcag tagaatcaga agtcagccag aagtctttgt
4561 ggaactacga ctacgctcca ccaccacctc cagagcagtg tctctctgat gatgaaatca
4621 cgatgatggc tcctgtggag tccaaatttt cccaatcaac tggagatata gataaagtga
4681 cagataccaa accaagagtg gctgagtggc gttatgggcc tgcccgactg tggtatgata
4741 tgctgggtgt ccctgaagat ggcagtgggt ttgactatgg cttcaaactg agaaagacag
4801 aacatgaacc tgtgataaaa tctagaatga tagaggaatt taggaaactt gaggaaaaca
4861 atggcactga tcttctggct gatgaaaact tcctgatggt gacacagctg cattgggagg
4921 atgatatcat ctgggatggg gaggatgtca aacacaaagg gacaaaacct cagcgtgcaa
4981 gcctggcagg ctggcttcct tctagcatga ctaggaatgc gatggcttac aatgttcagc
5041 aaggttttgc agccactctt gatgatgaca aaccttggta ctccattttt cccattgaca
5101 atgaggatct ggtatatgga cgctgggagg acaatatcat ttgggatgct caggccatgc
5161 cccggctgtt ggaacctcct gttttgacac ttgatcccaa tgatgagaac ctcattttgg
5221 aaattcctga tgagaaggaa gaggccacct ctaactcccc ctccaaggag agtaagaagg
5281 aatcatctct gaagaagagt cgaattctct tagggaaaac aggagtcatc aaggaggaac
5341 cacagcagaa catgtctcag ccagaagtga aagatccatg gaatctctcc aatgatgagt
5401 attattatcc caagcaacag ggtcttcgag gcacctttgg agggaatatt atccagcatt
5461 caattcctgc tgtggaatta cggcagccct tctttcccac ccacatgggg cccatcaaac
5521 tccggcagtt ccatcgccca cctctgaaaa agtactcatt tggtgcactt tctcagccag
5581 gtccccactc agtccaacct ttgctaaagc acatcaaaaa aaaggccaag atgagagaac
5641 aagagaggca agcttcaggt ggtggagaga tgttttttat gcgcacacct caggacctca
5701 caggcaaaga tggtgatctt attcttgcag aatatagtga ggaaaatgga cccttaatga
5761 tgcaggttgg catggcaacc aagataaaga actattataa acggaaacct ggaaaagatc
5821 ctggagcacc agattgtaaa tatggggaaa ctgtttactg ccatacatct cctttcctgg
5881 gttctctcca tcctggccaa ttgctgcaag catttgagaa caaccttttt cgtgctccaa
5941 tttatcttca taagatgcca gaaactgatt tcttgatcat tcggacaaga cagggttact
6001 atattcggga attagtggat atttttgtgg ttggccagca gtgtcccttg tttgaagttc
6061 ctgggcctaa ctccaaaagg gccaatacgc atattcgaga ctttctacag gtttttattt
6121 accgcctttt ctggaaaagt aaagatcggc cacggaggat acgaatggaa gatataaaaa
6181 aagcctttcc ttcccattca gaaagcagca tccggaagag gctaaagctc tgcgctgact
6241 tcaaacgcac agggatggac tcaaactggt gggtgcttaa gtctgatttt cgtttaccaa
6301 cggaagaaga gatcagagct atggtgtcac cagagcagtg ctgtgcttat tatagcatga
6361 tagctgcaga gcaacgactg aaggatgctg gctatggtga gaaatccttt tttgctccag
6421 aagaagaaaa tgaggaagat ttccagatga agattgatga tgaagttcgc actgcccctt
6481 ggaacaccac aagggccttc attgctgcca tgaagggcaa gtgtctgcta gaggtgactg
6541 gggtggcaga tcccacgggg tgtggtgaag gattctccta tgtgaagatt ccaaacaaac
6601 caacacagca gaaggatgat aaagaaccgc agccagtgaa gaagacagtg acaggaacag
6661 atgcagacct tcgtcgcctt tccctgaaaa atgccaagca acttctacgt aaatttggtg
6721 tgcctgagga agagattaaa aagttgtccc gctgggaagt gattgatgtg gtgcgcacaa
6781 tgtcaacaga acaggctcgt tctggagagg ggcccatgag taaatttgcc cgtggatcaa
6841 ggttttctgt ggctgagcat caagagcgtt acaaagagga atgtcagcgc atctttgacc
6901 tacagaacaa ggttctgtca tcaactgaag tcttatcaac tgacacagac agcagctcag
6961 ctgaagatag tgactttgaa gaaatgggaa agaacattga gaacatgttg cagaacaaga
7021 aaaccagctc tcagctttca cgtgaacggg aggaacagga gcggaaggaa ctacagcgaa
7081 tgctactggc agcaggctca gcagcatccg gaaacaatca cagagatgat gacacagctt
7141 ccgtgactag ccttaactct tctgccactg gacgctgtct caagatttat cgcacgtttc
7201 gagatgaaga ggggaaagag tatgttcgct gtgagacagt ccgaaaacca gctgtcattg
7261 atgcctatgt gcgcatacgg actacaaaag atgaggaatt cattcgaaaa tttgcccttt
7321 ttgatgaaca acatcgggaa gagatgcgaa aagaacggcg gaggattcaa gagcaactga
7381 ggcggcttaa gaggaaccag gaaaaggaga agcttaaggg tcctcctgag aagaagccca
7441 agaaaatgaa ggagcgtcct gacctaaaac tgaaatgtgg ggcatgtggt gccattggac
7501 acatgaggac taacaaattc tgccccctct attatcaaac aaatgcgcca ccttccaacc
7561 ctgttgccat gacagaagaa caggaggagg agttggaaaa gacagtcatt cataatgata
7621 atgaagaact tatcaaggtt gaagggacca aaattgtctt ggggaaacag ctaattgaga
7681 gtgcggatga ggttcgcaga aaatctctgg ttctcaagtt tcctaaacag cagcttcctc
7741 caaagaagaa acggcgagtt ggaaccactg ttcactgtga ctatttgaat agacctcata
7801 agtccatcca ccggcgccgc acagacccta tggtgacgct gtcgtccatc ttggagtcta
7861 tcatcaatga catgagagat cttccaaata catacccttt ccacactcca gtcaatgcaa
7921 aggttgtaaa ggactactac aaaatcatca ctcggccaat ggacctacaa acactccgcg
7981 aaaacgtgcg taaacgcctc tacccatctc gggaagagtt cagagagcat ctggagctaa
8041 ttgtgaaaaa tagtgcaacc tacaatgggc caaaacactc attgactcag atctctcaat
8101 ccatgctgga tctctgtgat gaaaaactca aagagaaaga agacaaatta gctcgcttag
8161 agaaagctat caaccccttg ctggatgatg atgaccaagt ggcgttttct ttcattctgg
8221 acaacattgt cacccagaaa atgatggcag ttccagattc ttggccattt catcacccag
8281 ttaataagaa atttgttcca gattattaca aagtgattgt caatccaatg gatttagaga
8341 ccatacgtaa gaacatctcc aagcacaagt atcagagtcg ggagagcttt ctggatgatg
8401 taaaccttat tctggccaac agtgttaagt ataatggacc tgagagtcag tatactaaga
8461 ctgcccagga gattgtgaac gtctgttacc agacattgac tgagtatgat gaacatttga
8521 ctcaacttga gaaggatatt tgtactgcta aagaagcagc tttggaggaa gcagaattag
8581 aaagcctgga cccaatgacc ccagggccct acacgcctca gcctcctgat ttgtatgata
8641 ccaacacatc cctcagtatg tctcgagatg cctctgtatt tcaagatgag agcaatatgt
8701 ctgtcttgga tattcccagt gccactccag aaaagcaggt aacacaggaa ggtgaagatg
8761 gagatggtga tcttgcagat gaagaggaag gaactgtaca acagcctcaa gccagtgtcc
8821 tgtatgagga tttgcttatg tctgaaggag aagatgatga ggaagatgct gggagtgatg
8881 aagaaggaga caatcctttc tctgctatcc agctgagtga aagtggaagt gactctgatg
8941 tgggatctgg tggaataaga cccaaacaac cccgcatgct tcaggagaac acaaggatgg
9001 acatggaaaa tgaagaaagc atgatgtcct atgagggaga cggtggggag gcttcccatg
9061 gtttggagga tagcaacatc agttatggga gctatgagga gcctgatccc aagtcgaaca
9121 cccaagacac aagcttcagc agcatcggtg ggtatgaggt atcagaggag gaagaagatg
9181 aggaggagga agagcagcgc tctgggccga gcgtactaag ccaggtccac ctgtcagagg
9241 acgaggagga cagtgaggat ttccactcca ttgctgggga cagtgacttg gactctgatg
9301 aattatcgcg gccgcactcg agatatctag acccagcttt cttgtacaaa gtggttgata
9361 acgggaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat
9421 gagtttaaac gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
9481 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
9541 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
9601 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
9661 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcagatctgc gcagctgggg
9721 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
9781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
9841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
9901 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
9961 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
10021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
10081 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
10141 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
10201 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
10261 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
10321 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
10381 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
10441 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
10501 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
10561 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
10621 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
10681 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
10741 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
10801 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
10861 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
10921 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
10981 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacttcgt ggccgaggag
11041 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
11101 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
11161 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
11221 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
11281 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
11341 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
11401 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
11461 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
11521 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgct
//
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GFP-TAF1质粒 GFPTAF1载体应用举例
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