| 质粒名称: | pT3-EF1A-mTert |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 162555 |
| 存储实验室: | Amaia Lujambio |
|
载体骨架基本信息 |
|
| 载体名称: | pT3 |
| 载体出品公司: | Xin Chen lab |
| 空载体大小: | 8500bp |
| 完整质粒大小: | 7967bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Overexpression of Tert |
| 物种来源: | M. musculus (mouse) |
| 基因突变: | 无 |
| 启动子: | EF1A |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | pT3-EF1A-mTert was a gift from Amaia Lujambio (Addgene plasmid # 162555 ; http://n2t.net/addgene:162555 ; RRID:Addgene_162555) |
| 参考文献部分: | Cooperation between distinct cancer driver genes underlies inter-tumor heterogeneity in hepatocellular carcinoma. Molina-Sanchez P, Ruiz de Galarreta M, Yao MA, Lindblad KE, Bresnahan E, Bitterman E, Martin TC, Rubenstein T, Nie K, Golas J, Choudhary S, Barcena-Varela M, Elmas A, Miguela V, Ding Y, Kan Z, Grinspan LT, Huang KL, Parsons RE, Shields DJ, Rollins RA, Lujambio A. Gastroenterology. 2020 Aug 16. pii: S0016-5085(20)35054-X. doi: 10.1053/j.gastro.2020.08.015. 10.1053/j.gastro.2020.08.015 PubMed 32814112 |
买家导航
pT3-EF1A-mTert载体质粒基本信息
pT3-EF1A-mTert质粒图谱载体图谱和pT3-EF1A-mTert载体序列质粒序列多克隆位点信息
pT3-EF1A-mTert质粒载体简介
pT3-EF1A-mTert质粒序列载体序列
LOCUS pT3-EF1A-mTert 7967 bp DNA circular SYN 14-APR-2025
DEFINITION Overexpression of Tert.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7967)
AUTHORS Molina-Sanchez P, Ruiz de Galarreta M, Yao MA, Lindblad KE,
Bresnahan E, Bitterman E, Martin TC, Rubenstein T, Nie K, Golas J,
Choudhary S, Barcena-Varela M, Elmas A, Miguela V, Ding Y, Kan Z,
Grinspan LT, Huang KL, Parsons RE, Shields DJ, Rollins RA, Lujambio
A
TITLE Cooperation between distinct cancer driver genes underlies
inter-tumor heterogeneity in hepatocellular carcinoma.
JOURNAL Gastroenterology. 2020 Aug 16. pii: S0016-5085(20)35054-X. doi:
10.1053/j.gastro.2020.08.015.
PUBMED 32814112
REFERENCE 2 (bases 1 to 7967)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Gastroenterology. 2020 Aug 16. pii: S0016-5085(20)35054-X. doi:
10.1053/j.gastro.2020.08.015."
COMMENT Sequence Label: pT3-EF1A-mTert
FEATURES Location/Qualifiers
source 1..7967
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(385..405)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
polyA_signal 4594..4715
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 4659..4678
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(5346..5362)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5346..5362)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5359..5381)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5370..5386
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(5394..5400,5401..5418,5419..5424))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5439..5460
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5577..5594)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5748..6336)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5828..5847)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6507..7298,7299..7367))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7130..7149
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7368..7472)
/gene="bla"
/label=AmpR promoter
primer_bind 7540..7558
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7596..7618)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7718..7737
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7931..7953
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7945..7962
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 7946..7962
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 aattcgagct cggtacccta cagttgaagt cggaagttta catacactta agttggagtc
61 attaaaactc gtttttcaac tactccacaa atttcttgtt aacaaacaat agttttggca
121 agtcagttag gacatctact ttgtgcatga cacaagtcat ttttccaaca attgtttaca
181 gacagattat ttcacttata attcactgta tcacaattcc agtgggtcag aagtttacat
241 acactaagtt gactgtgcct ttaaacagct tggaaaattc cagaaaatga tgtcatggct
301 ttagaagctt ctgatagact aattgacatc atttgagtca attggaggtg tacctgtgga
361 tgtatttcaa ggaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
421 gctccccagc aggcagaagt atgcaaagca tgcatttaat taaaattatc tctaaggcat
481 gtgaactggc tgtcttggtt ttcatctgta cttcatctgc tacctctgtg acctgaaaca
541 tatttataat tccattaagc tgtgcatatg atagatttat catatgtatt ttccttaaag
601 gatttttgta agaactaatt gaattgatac ctgtaaagtc tttatcacac tacccaataa
661 ataataaatc tctttgttca gctctctgtt tctataaata tgtaccagtt ttattgtttt
721 tagtggtagt gattttattc tctttctata tatatacaca cacatgtgtg cattcataaa
781 tatatacaat ttttatgaat aaaaaattat tagcaatcaa tattgaaaac cactgatttt
841 tgtttatgtg agcaaacagc agattaaaag gaatttcaat tgcctgcagg agtcaatggg
901 aaaaacccat tggagccaag tacactgact caatagggac tttccattgg gttttgccca
961 gtacataagg tcaatagggg gtgagtcaac aggaaagtcc cattggagcc aagtacattg
1021 agtcaatagg gactttccaa tgggttttgc ccagtacata aggtcaatgg gaggtaagcc
1081 aatgggtttt tcccattact gacatgtata ctgagtcatt agggactttc caatgggttt
1141 tgcccagtac ataaggtcaa taggggtgaa tcaacaggaa agtcccattg gagccaagta
1201 cactgagtca atagggactt tccattgggt tttgcccagt acaaaaggtc aatagggggt
1261 gagtcaatgg gtttttccca ttattggcac atacataagg tcaatagggg tgactagtgg
1321 agaagagcat gcttgagggc tgagtgcccc tcagtgggca gagagcacat ggcccacagt
1381 ccctgagaag ttggggggag gggtgggcaa ttgaactggt gcctagagaa ggtggggctt
1441 gggtaaactg ggaaagtgat gtggtgtact ggctccacct ttttccccag ggtgggggag
1501 aaccatatat aagtgcagta gtctctgtga acattcaagc ttctgccttc tccctcctgt
1561 gagtttggta agtcactgac tgtctatgcc tgggaaaggg tgggcaggag atggggcagt
1621 gcaggaaaag tggcactatg aaccctgcag ccctagacaa ttgtactaac cttcttctct
1681 ttcctctcct gacaggttgg tgtacagtag cttccaagta ctaagatcta gtgcacaggg
1741 cccaccatgg agctagctgg ggggatctca tgacccgcgc tcctcgttgc cccgcggtgc
1801 gctctctgct gcgcagccga taccgggagg tgtggccgct ggcaaccttt gtgcggcgcc
1861 tggggcccga gggcaggcgg cttgtgcaac ccggggaccc gaagatctac cgcactttgg
1921 ttgcccaatg cctagtgtgc atgcactggg gctcacagcc tccacctgcc gacctttcct
1981 tccaccaggt gtcatccctg aaagagctgg tggccagggt tgtgcagaga ctctgcgagc
2041 gcaacgagag aaacgtgctg gcttttggct ttgagctgct taacgaggcc agaggcgggc
2101 ctcccatggc cttcactagt agcgtgcgta gctacttgcc caacactgtt attgagaccc
2161 tgcgtgtcag tggtgcatgg atgctactgt tgagccgagt gggcgacgac ctgctggtct
2221 acctgctggc acactgtgct ctttatcttc tggtgccccc cagctgtgcc taccagggga
2281 gatggccaag agcgtctaaa cccctcattc ctactcagca acctccagcc taacttgact
2341 ggggccagga gactggtgga gatcatcttt ctgggctcaa ggcctaggac atcaggacca
2401 ctctgcagga cacaccgtct atcgcgtcga tactggcaga tgcggcccct gttccaacag
2461 ctgctggtga accatgcaga gtgccaatat gtcagactcc tcaggtcaca ttgcaggttt
2521 cgaacagcaa accaacaggt gacagatgcc ttgaacacca gcccaccgca cctcatggat
2581 ttgctccgcc tgcacagcag tccctggcag ggaaggaccg tgtccccgct gcagagcacc
2641 gtctgaggga gaggatcctg gctacgttcc tgttctggct gatggacaca tacgtggtac
2701 agctgcttag gtcattcttt tacatcacag agagcacatt ccagaagaac aggctcttct
2761 tctaccgtaa gagtgtgtgg agcaagctgc agagcattgg agtcaggcaa caccttgaga
2821 gagtgcggct acgggagctg tcacaagagg aggtcaggca tcaccaggac acctggctag
2881 ccatgcccat ctgcagactg cgcttcatcc ccaagcccaa cggcctgcgg cccattgtga
2941 acatgagtta tagcatgggt accagagctt tgggcagaag gaagcaggcc cagcatttca
3001 cccagcgtct caagactctc ttcagcatgc tcaactatga gcggacaaaa catcctcacc
3061 ttatggggtc ttctgtactg ggtatgaatg acatctacag gacctggcgg gcctttgtgc
3121 tgcgtgtgcg tgctctggac cagacaccca ggatgtactt tgttaaggca gatgtgaccg
3181 gggcctatga tgccatcccc cagggtaagc tggtggaggt tgttgccaat atgatcaggc
3241 actcggagag cacgtactgt atccgccagt atgcagtggt ccggagagat agccaaggcc
3301 aagtccacaa gtcctttagg agacaggtca ccaccctctc tgacctccag ccatacatgg
3361 gccagttcct taagcatctg caggattcag atgccagtgc actgaggaac tccgttgtca
3421 tcgagcagag catctctatg aatgagagca gcagcagcct gtttgacttc ttcctgcact
3481 tcctgcgtca cagtgtcgta aagattggtg acaggtgcta tacgcagtgc cagggcatcc
3541 cccagggctc cagcctatcc accctgctct gcagtctgtg tttcggagac atggagaaca
3601 agctgtttgc tgaggtgcag cgggatgggt tgcttttacg ttttgttgat gactttctgt
3661 tggtgacgcc tcacttggac caagcaaaaa ccttcctcag caccctggtc catggcgttc
3721 ctgagtatgg gtgcatgata aacttgcaga agacagtggt gaacttccct gtggagcctg
3781 gtaccctggg tggtgcagct ccataccagc tgcctgctca ctgcctgttt ccctggtgtg
3841 gcttgctgct ggacactcag actttggagg tgttctgtga ctactcaggt tatgcccaga
3901 cctcaattaa gacgagcctc accttccaga gtgtcttcaa agctgggaag accatgcgga
3961 acaagctcct gtcggtcttg cggttgaagt gtcacggtct atttctagac ttgcaggtga
4021 acagcctcca gacagtctgc atcaatatat acaagatctt cctgcttcag gcctacaggt
4081 tccatgcatg tgtgattcag cttccctttg accagcgtgt taggaagaac ctcacattct
4141 ttctgggcat catctccagc caagcatcct gctgctatgc tatcctgaag gtcaagaatc
4201 caggaatgac actaaaggcc tctggctcct ttcctcctga agccgcacat tggctctgct
4261 accaggcctt cctgctcaag ctggctgctc attctgtcat ctacaaatgt ctcctgggac
4321 ctctgaggac agcccaaaaa ctgctgtgcc ggaagctccc agaggcgaca atgaccatcc
4381 ttaaagctgc agctgaccca gccctaagca cagactttca gaccattttg gacatcagtg
4441 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
4501 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
4561 tgtaggtttg gcaagctagc tggccagaca tgataagata cattgatgag tttggacaaa
4621 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
4681 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
4741 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
4801 gtggtatgga attggagccc cactgtgttc atcttacaga tggaaatact gacattcaga
4861 ggagttagtt aacttgccta gcggccgctg cattctagtt gtggtttgtc caaactcatc
4921 aatgtatctt atcatgtctg gatccccttg aaatacatcc acaggtacac ctccaattga
4981 ctcaaatgat gtcaattagt ctatcagaag cttctaaagc catgacatca ttttctggaa
5041 ttttccaagc tgtttaaagg cacagtcaac ttagtgtatg taaacttctg acccactgga
5101 attgtgatac agtgaattat aagtgaaata atctgtctgt aaacaattgt tggaaaaatg
5161 acttgtgtca tgcacaaagt agatgtccta actgacttgc caaaactatt gtttgttaac
5221 aagaaatttg tggagtagtt gaaaaacgag ttttaatgac tccaacttaa gtgtatgtaa
5281 acttccgact tcaactgtat aggtctagag tcgacctgca ggcatgcaag cttggcgtaa
5341 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
5401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
5461 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
5521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
5581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
5641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
5701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
5761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
5821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
5881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
5941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
6001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
6061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
6121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
6181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
6241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
6301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
6361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
6421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
6481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
6541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
6601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
6661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
6721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
6781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
6841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
6901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
6961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
7021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
7081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
7141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
7201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
7261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
7321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
7381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
7441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
7501 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
7561 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
7621 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
7681 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
7741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
7801 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
7861 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
7921 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt
//
