| 质粒名称: | pLJC5-Tmem192-2xFlag |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 102929 |
| 存储实验室: | David Sabatini |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 载体出品公司: | pLJC5 |
| 空载体大小: | 8885bp |
| 完整质粒大小: | 8885 bp |
| 载体类型: | Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Tmem192 |
| 物种来源: | H. sapiens (human) |
| 启动子: | ubc |
| 标签/融合蛋白: | Flag (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI (not destroyed) |
| 3’克隆位点: | EcorI (not destroyed) |
| 5’测序引物 | CGAAGGAATAGAAGAAGAAGGTGGAGA |
|
文献引用方法 |
|
| 材料和方法部分: | pLJC5-Tmem192-2xFlag was a gift from David Sabatini (Addgene plasmid # 102929 ; http://n2t.net/addgene:102929 ; RRID:Addgene_102929) |
| 参考文献部分: | Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes. Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, Freinkman E, Sabatini DM. Science. 2017 Nov 10;358(6364):807-813. doi: 10.1126/science.aan6298. Epub 2017 Oct 26. 10.1126/science.aan6298 PubMed 29074583 |
买家导航
pLJC5-Tmem192-2xFlag载体质粒基本信息
pLJC5-Tmem192-2xFlag质粒图谱载体图谱和 pLJC5-Tmem192-2xFlag载体序列质粒序列多克隆位点信息
pLJC5-Tmem192-2xFlag质粒载体简介
pLJC5-Tmem192-2xFlag质粒序列载体序列
LOCUS pLJC5-Tmem192-2xFlag 8885 bp DNA circular SYN 14-APR-2025
DEFINITION Express a lysosomal Tag.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8885)
AUTHORS Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH,
Freinkman E, Sabatini DM
TITLE Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent
regulation of amino acid efflux from lysosomes.
JOURNAL Science. 2017 Nov 10;358(6364):807-813. doi:
10.1126/science.aan6298. Epub 2017 Oct 26.
PUBMED 29074583
REFERENCE 2 (bases 1 to 8885)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1126/science.aan6298"; journalName: "Science"; date:
"2017-11-10- 10"; volume: "358"; issue: "6364"; pages: "807-813"
COMMENT Sequence Label: pLJC5-Tmem192-2xFlag
FEATURES Location/Qualifiers
source 1..8885
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 435..560
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
LTR 607..787
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(788..1014)
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
primer_bind complement(1042..1062)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(1042..1060)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(1081..1097)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1081..1097)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1094..1116)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1105..1121
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(1129..1135,1136..1153,1154..1159))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1174..1195
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1312..1329)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1483..2071)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1563..1582)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(2242..3033,3034..3102))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2865..2884
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3103..3207)
/gene="bla"
/label=AmpR promoter
rep_origin complement(3233..3688)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3370..3391)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3582..3601
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3815..3837
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 3829..3846
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3830..3846
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3856..3875
/label=T7
/note="T7 promoter, forward primer"
promoter 3856..3874
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 3895..4030
/label=SV40 ori
/note="SV40 origin of replication"
polyA_signal 4070..4191
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4081..4100)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 4135..4154
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
LTR 4263..4496
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
CDS complement(4624..5223)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4707..4727)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 5204..5223
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
promoter complement(5245..5755)
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind complement(5308..5326)
/label=hPGK-F
/note="Human PGK promoter, forward primer"
misc_feature 5804..5921
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(5966..6013)
/codon_start=1
/product="two tandem FLAG(R) epitope tags"
/label=2xFLAG
/translation="DYKDDDDKDYKDDDDK"
regulatory 6834..6843
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
promoter complement(6865..8076)
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind complement(7009..7028)
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS complement(8365..8409)
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8594..8827
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
ORIGIN
1 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
61 tcatatctcc tcctccaggt ctgaagatca gctgctgctt gctgtgcggt ggtcttactt
121 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
181 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
241 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
301 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
361 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
421 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
481 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
541 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
601 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
661 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
721 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
781 gagacccgtt tatgtatcga gctaggcact taaatacaat atctctgcaa tgcggcaatt
841 cagtggttcg tccaatccat gtcagacccg tctgttgcct tcctaataag gcacgatcgt
901 accaccttac ttccaccaat cggcatgcac ggtgcttttt ctctccttgt aaggcatgtt
961 gctaactcat cgttaccatg ttgcaagact acaagagtat tgcataagac tacattaagc
1021 ttgcagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
1081 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
1141 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
1201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
1261 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
1321 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
1381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
1441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
1501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
1561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
1621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
1681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
1741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
1801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
1861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
1921 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
1981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
2041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
2101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
2161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
2221 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
2281 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
2341 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
2401 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
2461 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
2521 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
2581 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
2641 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
2701 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
2761 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
2821 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
2881 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
2941 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
3001 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
3061 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
3121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
3181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
3241 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
3301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
3361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
3421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
3481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
3541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
3601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
3661 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
3721 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
3781 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
3841 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacgtc cctaggcctc
3901 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
3961 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
4021 ggggcgggat gggcggagtt aggggcggga tagctagagc cagacatgat aagatacatt
4081 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
4141 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcctctcact
4201 ctctgatatt catttctttg caagttataa atactgaata ataagatgac atgaactact
4261 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
4321 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
4381 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
4441 cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc
4501 ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
4561 aggtaccgat gcatggggtc gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg
4621 gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
4681 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
4741 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
4801 gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc
4861 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
4921 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
4981 gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc
5041 gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg
5101 gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt
5161 gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt
5221 catggtaagc tccggtggat ccccctgggg agagaggtcg gtgattcggt caacgaggga
5281 gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa
5341 cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc ggccgctgct
5401 ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc ggcgcgctgc
5461 cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg
5521 tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg gagcaggaag
5581 cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc gaacggacgt
5641 gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca
5701 gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca accccctcga
5761 gccgcggcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
5821 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
5881 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
5941 tttgtctcga ggtcgagaat tcttacttgt cgtcatcgtc tttgtagtcc ttgtcgtcat
6001 cgtctttgta gtcactacct ccacctcccg ttctacttgg ctgacagccc aggtctgagg
6061 aagtgagagc caacaatcgc ttactcagca gcgcattgtg tcgcttcagg tattcaatgg
6121 tgtctccttg cttttcaaca atttcttcta ggcttgaaat agttctgaat ccagtctccg
6181 aggtaatatt gctggggtaa gcatagattt tttcttcttc aagtatatca ggctctggtt
6241 tagctttatt aaatctccgg attttcactg tgtaaatgag gagacatatc agggaacaga
6301 tgagttccag tgccaagatg gccagaatga ggtcaagata caatctgcca ggctctggga
6361 aggagtgctg catgcacagt atgaggagaa gcactgtgtt gccagaggac tgtatcatca
6421 acgcaagtct cttgagatgc cttgttgatc ggtagatcaa gttatagcct cggtttctga
6481 ttttgctgtg gtgatactgg atgtagcatt caaggagtaa atggagaatc cacaaaataa
6541 ctttcccaag gattataacc gtctgaactt tcaatgggtt tgtgtaattt cctgggcact
6601 tgtcctcatt tggattagga taagaacaaa gcacacctgt taaaaatgct aaaacaacaa
6661 acacgagatg aataaaccac agaagattca ctatgatgac tgtaggaaga ggatggaatc
6721 ggggtctaaa gtgagcttgt aatgagtggt gtgggagaag ctgggcatcc agaagtgggt
6781 cgtcttcaat actctgggtg atatccaagg aaccgtcctc catcctgccc cccgccgcca
6841 tggtaccggt agcgctagcg cttcgtctaa caaaaaagcc aaaaacggcc agaatttagc
6901 ggacaattta ctagtctaac actgaaaatt acatattgac ccaaatgatt acatttcaaa
6961 aggtgcctaa aaaacttcac aaaacacact cgccaacccc gagcgcatag ttcaaaaccg
7021 gagcttcagc tacttaagaa gataggtaca taaaaccgac caaagaaact gacgcctcac
7081 ttatccctcc cctcaccaga ggtccggcgc ctgtcgattc aggagagcct accctaggcc
7141 cgaaccctgc gtcctgcgac ggagaaaagc ctaccgcaca cctaccggca ggtggcccca
7201 ccctgcatta taagccaaca gaacgggtga cgtcacgaca cgacgagggc gcgcgctccc
7261 aaaggtacgg gtgcactgcc caacggcacc gccataactg ccgcccccgc aacagacgac
7321 aaaccgagtt ctccagtcag tgacaaactt cacgtcaggg tccccagatg gtgccccagc
7381 ccatctcacc cgaataagag ctttcccgca ttagcgaagg cctcaagacc ttgggttctt
7441 gccgcccacc atgcccccca ccttgtttca acgacctcac agcccgcctc acaagcgtct
7501 tccattcaag actcgggaac agccgccatt ttgctgcgct ccccccaacc cccagttcag
7561 ggcaaccttg ctcgcggacc cagactacag cccttggcgg tctctccaca cgcttccgtc
7621 ccaccgagcg gcccggcggc cacgaaagcc ccggccagcc cagcagcccg ctactcacca
7681 agtgacgatc acagcgatcc acaaacaaga accgcgaccc aaatcccggc tgcgacggaa
7741 ctagctgtgc cacacccggc gcgtccttat ataatcatcg gcgttcaccg ccccacggag
7801 atccctccgc agaatcgccg agaagggact acttttcctc gcctgttccg ctctctggaa
7861 agaaaaccag tgccctagag tcacccaagt cccgtcctaa aatgtccttc tgctgatact
7921 ggggttctaa ggccgagtct tatgagcagc gggccgctgt cctgagcgtc cgggcggaag
7981 gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc gctcgccgtg aggagggggg
8041 cgcccgcggg aggcgccaaa acccggcgcg gaggccagat cccgctcgag gctagtctcg
8101 tgatcgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
8161 accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg
8221 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
8281 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
8341 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
8401 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
8461 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
8521 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
8581 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
8641 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
8701 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
8761 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
8821 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
8881 tgcta
//
