| 质粒名称: | pfwB |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 37329 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 3729 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Zeocin, Blasticidin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | Aequorea victoria |
| 启动子: | EF1a |
| 克隆方法: | 未知 |
| 5’克隆位点: | N/A |
| 3’克隆位点: | N/A |
|
文献引用方法 |
|
| 材料和方法部分: | pfwB was a gift from John Schiller (Addgene plasmid # 37329 ; http://n2t.net/addgene:37329 ; RRID:Addgene_37329) |
| 参考文献部分: | Maturation of papillomavirus capsids. Buck CB, Thompson CD, Pang YY, Lowy DR, Schiller JT. J Virol. 2005 Mar;79(5):2839-46. 10.1128/JVI.79.5.2839-2846.2005 PubMed 15709003 |
买家导航
pfwB载体质粒基本信息
pfwB质粒图谱载体图谱和pfwB载体序列质粒序列多克隆位点信息
pfwB质粒载体简介
pfwB质粒序列载体序列
LOCUS pfwB 5904 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5904)
AUTHORS Buck CB, Thompson CD, Pang YY, Lowy DR, Schiller JT
TITLE Maturation of papillomavirus capsids.
JOURNAL J Virol. 2005 Mar;79(5):2839-46.
PUBMED 15709003
REFERENCE 2 (bases 1 to 5904)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Virol.";
date: "2005-03"; volume: "79(5)"; pages: "2839-46"
COMMENT Sequence Label: pfwB
FEATURES Location/Qualifiers
source 1..5904
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(53..73)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 58..387
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 238..373
/label=SV40 ori
/note="SV40 origin of replication"
promoter 435..482
/label=EM7 promoter
/note="synthetic bacterial promoter "
polyA_signal 962..1083
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(973..992)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1027..1046
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
promoter 1265..2443
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 1496..2434
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 2391..2411
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 2554..2563
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(2560..2562,2563..2565,2566..3279)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(2560..2562,2563..2565,2566..3276)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2605..2626)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2866..2885)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3213..3234
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 3309..3325
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3309..3325
/label=pBluescriptSK
/note="For pBluescript vector"
misc_feature 3363..3951
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3416..3436)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3834..3845)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3993..4565
/label=EF-1-alpha poly(A) signal
/note="human EF-1-alpha polyadenylation signal"
rep_origin 4736..5324
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5225..5244
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 5410..5457
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter 5449..5467
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ccctgcaggg cctgaaataa cctctgaaag aggaacttgg ttaggtacct gtggaatgtg
61 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
121 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
181 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
241 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
301 atttatacag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
361 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
421 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
481 acaaggtgag gaactaaatc atgaagacct tcaacatctc tcagcaggat ctggagctgg
541 tggaggtcgc cactgagaag atcaccatgc tctatgagga caacaagcac catgtcgggg
601 cggccatcag gaccaagact ggggagatca tctctgctgt ccacattgag gcctacattg
661 gcagggtcac tgtctgtgct gaagccattg ccattgggtc tgctgtgagc aacgggcaga
721 aggactttga caccattgtg gctgtcaggc acccctactc tgatgaggtg gacagatcca
781 tcagggtggt cagcccctgt ggcatgtgca gagagctcat ctctgactat gctcctgact
841 gctttgtgct cattgagatg aatggcaagc tggtcaaaac caccattgag gaactcatcc
901 ccctcaagta caccaggaac taaacctgaa ttcgctagag ggccgcttcg agcagacatg
961 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
1021 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
1081 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
1141 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gggctggcgt
1201 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcggtggag aagagcatgc
1261 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
1321 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
1381 aagtgatgtc gtgtactggc tccgcccttt tcccgagggt gggggagaac cgtatataag
1441 tgcagtagtc gctgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
1501 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
1561 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
1621 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
1681 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
1741 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
1801 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
1861 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
1921 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
1981 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
2041 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
2101 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
2161 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
2221 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
2281 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
2341 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
2401 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaattc tctagagatc
2461 cctcgacctc gagatccatt gtgctggatc tgcgatctaa gtaagcttcg aattctgcag
2521 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
2581 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
2641 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
2701 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
2761 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
2821 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
2881 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
2941 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
3001 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
3061 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
3121 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
3181 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
3241 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgctc caccgcggtg
3301 gcggccgctc tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg
3361 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
3421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
3481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
3541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
3601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
3661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
3721 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
3781 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
3841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
3901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
3961 gtcggcccac tgctccctaa acctgagcta gcattatccc taatacctgc caccccactc
4021 ttaatcagtg gtggaagaac ggtctcagaa ctgtttgttt caattggcca tttaagttta
4081 gtagtaaaag actggttaat gataacaatg catcgtaaaa ccttcagaag gaaaggagaa
4141 tgttttgtgg accactttgg ttttcttttt tgcgtgtggc agttttaagt tattagtttt
4201 taaaatcagt actttttaat ggaaacaact tgaccaaaaa tttgtcacag aattttgaga
4261 cccattaaaa aagttaaatg agaaacctgt gtgttccttt ggtcaacacc gagacattta
4321 ggtgaaagac atctaattct ggttttacga atctggaaac ttcttgaaaa tgtaattctt
4381 gagttaacac ttctgggtgg agaatagggt tgttttcccc ccacataatt ggaaggggaa
4441 ggaatatcat ttaaagctat gggagggttt ctttgattac aacactggag agaaatgcag
4501 catgttgctg attgcctgtc actaaaacag gccaaaaact gagtccttgg gttgcataga
4561 aagcttcatg ttgctaaacc aatgttaagt gaatctttgg aaacaaaatg tttccaaatt
4621 actgggatgt gcatgttgaa acgtgggtta attaactagc catgaccaaa atcccttaac
4681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
4741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
4801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
4861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
4921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
4981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
5041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
5101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
5161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
5221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
5281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
5341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttctta attaaatttt
5401 tcaaaagtag ttgacaatta atcatcggca tagtatatcg gcatagtata atacgactca
5461 ctataggagg gccatcatgg ccaagttgac cagtgctgtc ccagtgctca cagccaggga
5521 tgtggctgga gctgttgagt tctggactga caggttgggg ttctccagag attttgtgga
5581 ggatgacttt gcaggtgtgg tcagagatga tgtcaccctg ttcatctcag cagtccagga
5641 ccaggtggtg cctgacaaca ccctggcttg ggtgtgggtg agaggactgg atgagctgta
5701 tgctgagtgg agtgaggtgg tctccaccaa cttcagggat gccagtggcc ctgccatgac
5761 agagattgga gagcagccct gggggagaga gtttgccctg agagacccag caggcaactg
5821 tgtgcacttt gtggcagagg agcaggactg aggataagaa ttgtaacaaa aaaccccgcc
5881 ccggcggggt tttttgttaa ttaa
//
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