PCDH-TetO-mEGFP-TRIM1-TurboID

LOCUS      PCDH-TetO-mEGFP-TRIM1-TurboID               10394 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10394)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1980..2250
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2283..2320
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2444..3160
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2444..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3161..3190
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3197..5401
                     /label=TRIM1
     misc_feature    3284..3433
                     /label=RING 30-80
     CDS             5420..5446
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5450..5476
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5480..5506
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5507..6487
                     /codon_start=1
                     /label=TurboID
                     /translation="ASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEKEFSRAD"
     misc_feature    6491..7078
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6962..6973)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7151..7384
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7456..7577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7596..7731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7769..7785)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7793..7809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7817..7847)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7862..7883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8171..8759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8930..9790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9791..9895)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10369..10385
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtcgg gtttattaca gggacagcag agatccagtt tggttatcga gtttaccact
     1981 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2041 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2101 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2161 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2221 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
     2281 gagtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2341 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2401 cctccgcggc ccctctagac cgctagcgct accggtcgcc accatggtga gcaagggcga
     2461 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2521 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2581 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2641 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2701 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2761 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2821 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2881 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2941 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3001 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3061 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3121 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc
     3181 tcaagcttcg gaattcatgg gtgaaagccc agcctccgtg gttcttaatg cctcaggagg
     3241 actattttca ctaaagatgg aaacactgga gtctgaattg acctgtccaa tctgcctaga
     3301 gttgtttgaa gacccccttc tgctcccttg tgctcacagc ctctgcttca gctgtgccca
     3361 tcgcattttg gtatcaagct gcagctctgg tgaatccatt gaacccatta ctgctttcca
     3421 gtgtcctacc tgcaggtatg ttatctcgct gaaccaccgg ggcctggatg gcctcaagag
     3481 gaatgtgact ctgcagaaca ttattgatcg cttccagaag gcttcagtca gtgggcccaa
     3541 ttcccctagt gagagccgcc gggaaaggac ttacaggccc accactgcca tgtctagcga
     3601 gcgaattgct tgccaattct gtgagcagga cccgccaagg gatgcagtaa aaacatgcat
     3661 cacctgtgag gtctcctact gtgaccgttg cctgcgggcc acgcacccca acaagaaacc
     3721 tttcaccagc caccgcctgg tggaaccagt gccagacaca catcttcgag ggatcacctg
     3781 cctggaccat gagaatgaga aagtgaacat gtactgtgta tctgatgacc aattgatctg
     3841 tgccttatgc aaactggtgg gtcgtcaccg agaccatcag gtcgcatccc tgaatgatcg
     3901 atttgagaaa ctcaagcaaa ctctggagat gaacctcacc aacctggtta agcgcaacag
     3961 cgaactagaa aatcaaatgg ccaaactaat acagatctgc cagcaggttg aggtgaatac
     4021 tgctatgcat gaggcaaaac ttatggaaga atgtgacgag ttggtagaga tcatccagca
     4081 gaggaagcaa atgatcgctg tcaaaatcaa agagacaaag gttatgaaac tgagaaagtt
     4141 ggcacagcag gttgctaatt gccgccagtg tcttgaacgg tcaacagtcc tcatcaacca
     4201 agctgagcat atcctgaaag aaaatgacca ggcacggttt ctacagtctg caaaaaatat
     4261 tgctgagagg gtcgctatgg caactgcatc ttctcaagtt ctgattccag acatcaattt
     4321 taatgatgcc tttgaaaact ttgctttaga tttttccaga gaaaagaaac tgctagaggg
     4381 gttagattat ttaacagccc caaacccacc atctatccga gaagaactct gtactgcctc
     4441 ccatgacacc attacagtcc actggatctc ggatgatgag ttcagcatca gctcctatga
     4501 gcttcagtac accatattca ctggccaggc taacttcatc agtaagtcat ggtgtagttg
     4561 gggcctgtgg ccagagataa ggaaatgtaa ggaagcagta agctgctcaa gattggccgg
     4621 ggcgccacga ggcctgtata attcagtaga cagctggatg attgttccca acattaaaca
     4681 gaaccattac acagtgcatg gactccagag cgggactcgc tacatcttca tcgttaaagc
     4741 cataaaccaa gccggcagcc ggaacagtga acctacccga ctaaaaacaa acagccaacc
     4801 ctttaaattg gatcccaaaa tgactcacaa gaagttgaag atctccaatg atggattgca
     4861 gatggagaag gatgaaagct ctctaaagaa gagccacacc ccagagaggt ttagtggcac
     4921 agggtgctat ggggcagcag gaaatatatt cattgacagt ggctgccact attgggaggt
     4981 ggtcatgggt tcctcaacat ggtatgcaat tggcattgcc tacaaatcag ctccaaagaa
     5041 tgaatggatt ggcaagaatg cctcctcatg ggtcttctct cgctgcaata gtaacttcgt
     5101 ggtgagacac aacaacaagg aaatgctggt ggatgtgccc ccacacctga agcgtctggg
     5161 tgtcctcctg gattatgaca acaatatgct gtctttctat gacccagcta actctctcca
     5221 tcttcatact tttgatgtga ccttcattct tccagtttgt ccaacattta caatctggaa
     5281 caaatcccta atgatcctgt ctggcttgcc tgccccagat tttattgatt accctgagcg
     5341 gcaggaatgc aactgcaggc ctcaagaatc cccttatgtt tctgggatga aaacctgtca
     5401 tggatccgga tctggctcct acccgtatga tgttccggat tacgctggct atccctacga
     5461 cgtgcccgac tatgccgggt acccctatga cgtcccagac tacgcagcta gcaaagacaa
     5521 tactgtgcct ctgaagctga tcgctctcct ggctaatggc gagttccata gtggcgaaca
     5581 gctgggagaa accctgggca tgtccagggc cgctatcaac aagcacattc agactctgcg
     5641 cgactggggc gtggacgtgt tcaccgtgcc cggaaagggc tactctctgc ccgagcctat
     5701 cccgctgctg aacgctaaac agattctggg acagctggac ggcgggagcg tggcagtcct
     5761 gcctgtggtc gactccacca atcagtacct gctggatcga atcggcgagc tgaagagtgg
     5821 ggatgcttgc attgcagaat atcagcaggc agggagagga agcagaggga ggaaatggtt
     5881 ctctcctttt ggagctaacc tgtacctgag tatgttttgg cgcctgaagc ggggaccagc
     5941 agcaatcggc ctgggcccgg tcatcggaat tgtcatggca gaagcgctgc gaaagctggg
     6001 agcagacaag gtgcgagtca aatggcccaa tgacctgtat ctgcaggata gaaagctggc
     6061 aggcatcctg gtggagctgg ccggaataac aggcgatgct gcacagatcg tcattggcgc
     6121 cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac
     6181 actgcaggaa gcagggatta acctggacag gaatactctg gccgctacgc tgatccgaga
     6241 gctgcgggca gccctggaac tgttcgagca ggaaggcctg gctccatatc tgccacggtg
     6301 ggagaagctg gataacttca tcaatagacc cgtgaagctg atcattgggg acaaagagat
     6361 tttcgggatt agccggggga ttgataaaca gggagccctg ctgctggaac aggacggagt
     6421 tatcaaaccc tggatgggcg gagaaatcag tctgcggtct gccgaaaagg agttcagcag
     6481 ggccgactga aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6541 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6601 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6661 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6721 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6781 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6841 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     6901 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6961 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7021 tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgccg
     7081 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7141 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     7201 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7261 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7321 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7381 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7441 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7501 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7561 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     7621 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7681 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     7741 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7801 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7861 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7921 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7981 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8041 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8101 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8161 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8221 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8281 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8341 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8401 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8461 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8521 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8581 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8641 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8701 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8761 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8821 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8881 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8941 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9001 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9061 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9121 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9181 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9241 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9301 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9361 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9421 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9481 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9541 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9601 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9661 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9721 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9781 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9841 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9901 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     9961 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10021 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10081 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    10141 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10201 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    10261 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10321 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10381 ccagtgccaa gctg
//