| 质粒名称: | pAAVLINK-3'-ATRX-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001923 |
| 产品用途: | 3' AAVLINK plasmid for ATRX expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ATRX |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-ATRX-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-ATRX-FLAG载体质粒基本信息
pAAVLINK-3'-ATRX-FLAG质粒图谱载体图谱和pAAVLINK-3'-ATRX-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-ATRX-FLAG质粒载体简介
pAAVLINK-3'-ATRX-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-ATRX-FLAG 7271 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7271)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7271
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4010
/codon_start=1
/gene="ATRX"
/gene_synonym="JMS; MRX52; RAD54; RAD54L; XH2; XNP;ZNF-HX"
/product="transcriptional regulator ATRX isoform 1"
/EC_number="3.6.4.12"
/label=ATRX
/note="isoform 1 is encoded by transcript variant
1;X-linked nuclear protein; transcriptional regulator ATRX;
X-linked helicase II; ATP-dependent helicase ATRX"
/db_xref="GI:530788276"
/db_xref="CCDS:CCDS14434.1"
/db_xref="GeneID:546"
/db_xref="HGNC:HGNC:886"
/db_xref="MIM:300032"
/protein_id="NP_000480.3"
/translation="DEALSKSVPVTVDDDDDDNDPENRIAKKMLLEEIKANLSSDEDGS
SDDEPEEGKKRTGKQNEENPGDEEAKNQVNSESDSDSEESKKPRYRHRLLRHKLTVSDG
ESGEEKKTKPKEHKEVKGRNRRKVSSEDSEDSDFQESGVSEEVSESEDEQRPRTRSAKK
AELEENQRSYKQKKKRRRIKVQEDSSSENKSNSEEEEEEKEEEEEEEEEEEEEEEDEND
DSKSPGKGRKKIRKILKDDKLRTETQNALKEEEERRKRIAEREREREKLREVIEIEDAS
PTKCPITTKLVLDEDEETKEPLVQVHRNMVIKLKPHQVDGVQFMWDCCCESVKKTKKSP
GSGCILAHCMGLGKTLQVVSFLHTVLLCDKLDFSTALVVCPLNTALNWMNEFEKWQEGL
KDDEKLEVSELATVKRPQERSYMLQRWQEDGGVMIIGYEMYRNLAQGRNVKSRKLKEIF
NKALVDPGPDFVVCDEGHILKNEASAVSKAMNSIRSRRRIILTGTPLQNNLIEYHCMVN
FIKENLLGSIKEFRNRFINPIQNGQCADSTMVDVRVMKKRAHILYEMLAGCVQRKDYTA
LTKFLPPKHEYVLAVRMTSIQCKLYQYYLDHLTGVGNNSEGGRGKAGAKLFQDFQMLSR
IWTHPWCLQLDYISKENKGYFDEDSMDEFIASDSDETSMSLSSDDYTKKKKKGKKGKKD
SSSSGSGSDNDVEVIKVWNSRSRGGGEGNVDETGNNPSVSLKLEESKATSSSNPSSPAP
DWYKDFVTDADAEVLEHSGKMVLLFEILRMAEEIGDKVLVFSQSLISLDLIEDFLELAS
REKTEDKDKPLIYKGEGKWLRNIDYYRLDGSTTAQSRKKWAEEFNDETNVRGRLFIIST
KAGSLGINLVAANRVIIFDASWNPSYDIQSIFRVYRFGQTKPVYVYRFLAQGTMEDKIY
DRQVTKQSLSFRVVDQQQVERHFTMNELTELYTFEPDLLDDPNSEKKKKRDTPMLPKDT
ILAELLQIHKEHIVGYHEHDSLLDHKEEEELTEEERKAAWAEYEAEKKGLTMRFNIPTG
TNLPPVSFNSQTPYIPFNLGALSAMSNQQLEDLINQGREKVVEATNSVTAVRIQPLEDI
ISAVWKENMNLSEAQVQALALSRQASQELDVKRREAIYNDVLTKQQMLISCVQRILMNR
RLQQQYNQQQQQQMTYQQATLGHLMMPKPPNLIMNPSNYQQIDMRGMYQPVAGGMQPPP
LQRAPPPMRSKNPGPSQGKSM"
misc_feature 871..876
/label=SDS
misc_feature 886..891
/label=SDS
misc_feature 889..894
/label=SDS
misc_feature 898..903
/label=SDS
misc_feature 901..906
/label=SDS
misc_feature 904..909
/label=SDS
misc_feature 907..912
/label=SDS
misc_feature 916..921
/label=SDS
misc_feature 919..924
/label=SDS
misc_feature 1755..1760
/label=SDS
misc_feature 1758..1763
/label=SDS
CDS 4017..4082
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4086..4517
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4230..4235
/label=SDS
repeat_region 4534..4674
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4534..4663
/label=AAV2 ITR
repeat_region 4535..4675
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4749..5204
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5486..5590
/gene="bla"
/label=AmpR promoter
CDS 5591..6451
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6622..7210
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gagatgaagc cttatctaaa tcagtgcctg
301 tcacagtgga tgatgatgat gacgacaatg atcctgagaa tagaattgcc aagaagatgc
361 ttttagaaga aattaaagcc aatctttcct ctgatgagga tggatcttca gatgatgagc
421 cagaagaagg gaaaaaaaga actggaaaac aaaatgaaga aaacccagga gatgaggaag
481 caaaaaatca agtcaattct gaatcagatt cagattctga agaatctaag aagccaagat
541 acagacatag gcttttgcgg cacaaattga ctgtgagtga cggagaatct ggagaagaaa
601 aaaagacaaa gcctaaagag cataaagaag tcaaaggcag aaacagaaga aaggtgagca
661 gtgaagattc agaagattct gattttcagg aatcaggagt tagtgaagaa gttagtgaat
721 ccgaagatga acagcggccc agaacaaggt ctgcaaagaa agcagagttg gaagaaaatc
781 agcggagcta taaacagaaa aagaaaaggc gacgtattaa ggttcaagaa gattcatcca
841 gtgaaaacaa gagtaattct gaggaagaag aggaggaaaa agaagaggag gaggaagagg
901 aggaggagga ggaagaggag gaggaagatg aaaatgatga ttccaagtct cctggaaaag
961 gcagaaagaa aattcggaag attcttaaag atgataaact gagaacagaa acacaaaatg
1021 ctcttaagga agaggaagag agacgaaaac gtattgctga gagggagcgt gagcgagaaa
1081 aattgagaga ggtgatagaa attgaagatg cttcacccac caagtgtcca ataacaacca
1141 agttggtttt agatgaagat gaagaaacca aagaaccttt agtgcaggtt catagaaata
1201 tggttatcaa attgaaaccc catcaagtag atggtgttca gtttatgtgg gattgctgct
1261 gtgagtctgt gaaaaaaaca aagaaatctc caggttcagg atgcattctt gcccactgta
1321 tgggccttgg taagacttta caggtggtaa gttttcttca tacagttctt ttgtgtgaca
1381 aactggattt cagcacggcg ttagtggttt gtcctcttaa tactgctttg aattggatga
1441 atgaatttga gaagtggcaa gagggattaa aagatgatga gaagcttgag gtttctgaat
1501 tagcaactgt gaaacgtcct caggagagaa gctacatgct gcagaggtgg caagaagatg
1561 gtggtgttat gatcataggc tatgagatgt atagaaatct tgctcaagga aggaatgtga
1621 agagtcggaa acttaaagaa atatttaaca aagctttggt tgatccaggc cctgattttg
1681 ttgtttgtga tgaaggccat attctaaaaa atgaagcatc tgctgtttct aaagctatga
1741 attctatacg atcaaggagg aggattattt taacaggaac accacttcaa aataacctaa
1801 ttgagtatca ttgtatggtt aattttatca aggaaaattt acttggatcc attaaggagt
1861 tcaggaatag atttataaat ccaattcaaa atggtcagtg tgcagattct accatggtag
1921 atgtcagagt gatgaaaaaa cgtgctcaca ttctctatga gatgttagct ggatgtgttc
1981 agaggaaaga ttatacagca ttaacaaaat tcttgcctcc aaaacacgaa tatgtgttag
2041 ctgtgagaat gacttctatt cagtgcaagc tctatcagta ctacttagat cacttaacag
2101 gtgtgggcaa taatagtgaa ggtggaagag gaaaggcagg tgcaaagctt ttccaagatt
2161 ttcagatgtt aagtagaata tggactcatc cttggtgttt gcagctagac tacattagca
2221 aagaaaataa gggttatttt gatgaagaca gtatggatga atttatagcc tcagattctg
2281 atgaaacctc catgagttta agctccgatg attatacaaa aaagaagaaa aaagggaaaa
2341 aggggaaaaa agatagtagc tcaagtggaa gtggcagtga caatgatgtt gaagtgatta
2401 aggtctggaa ttcaagatct cggggaggtg gtgaaggaaa tgtggatgaa acaggaaaca
2461 atccttctgt ttctttaaaa ctggaagaaa gtaaagctac ttcttcttct aatccaagca
2521 gcccagctcc agactggtac aaagattttg ttacagatgc tgatgctgag gttttagagc
2581 attctgggaa aatggtactt ctctttgaaa ttcttcgaat ggcagaggaa attggggata
2641 aagtccttgt tttcagccag tccctcatat ctctggactt gattgaagat tttcttgaat
2701 tagctagtag ggagaagaca gaagataaag ataaacccct tatttataaa ggtgagggga
2761 agtggcttcg aaacattgac tattaccgtt tagatggttc cactactgca cagtcaagga
2821 agaagtgggc tgaagaattt aatgatgaaa ctaatgtgag aggacgatta tttatcattt
2881 ctactaaagc aggatctcta ggaattaatc tggtagctgc taatcgagta attatattcg
2941 acgcttcttg gaatccatct tatgacatcc agagtatatt cagagtttat cgctttggac
3001 aaactaagcc tgtttatgta tataggttct tagctcaggg aaccatggaa gataagattt
3061 atgatcggca agtaactaag cagtcactgt cttttcgagt tgttgatcag cagcaggtgg
3121 agcgtcattt tactatgaat gagcttactg aactttatac ttttgagcca gacttattag
3181 atgaccctaa ttcagaaaag aagaagaaga gggatactcc catgctgcca aaggatacca
3241 tacttgcaga gctccttcag atacataaag aacacattgt aggataccat gaacatgatt
3301 ctcttttgga ccacaaagaa gaagaagagt tgactgaaga agaaagaaaa gcagcttggg
3361 ctgagtatga agcagagaag aagggactga ccatgcgttt caacatacca actgggacca
3421 atttaccccc tgtcagtttc aactctcaaa ctccttatat tcctttcaat ttgggagccc
3481 tgtcagcaat gagtaatcaa cagctggagg acctcattaa tcaaggaaga gaaaaagttg
3541 tagaagcaac aaacagtgtg acagcagtga ggattcaacc tcttgaggat ataatttcag
3601 ctgtatggaa ggagaacatg aatctctcag aggcccaagt acaggcgtta gcattaagta
3661 gacaagccag ccaggagctt gatgttaaac gaagagaagc aatctacaat gatgtattga
3721 caaaacaaca gatgttaatc agctgtgttc agcgaatact tatgaacaga aggctccagc
3781 agcagtacaa tcagcagcaa cagcaacaaa tgacttatca acaagcaaca ctgggtcacc
3841 tcatgatgcc aaagccccca aatttgatca tgaatccttc taactaccag cagattgata
3901 tgagaggaat gtatcagcca gtggctggtg gtatgcagcc accaccatta cagcgtgcac
3961 cacccccaat gagaagcaaa aatccaggac cttcccaagg gaaatcaatg tgtacagact
4021 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
4081 agtaactcga gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
4141 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
4201 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc
4261 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
4321 cactgacaat tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac
4381 catctagctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
4441 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
4501 tgtgggaggt tttttaacgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
4561 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
4621 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
4681 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
4741 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4801 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
4861 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4921 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
4981 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
5041 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
5101 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
5161 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
5221 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
5281 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
5341 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
5401 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
5461 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
5521 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
5581 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
5641 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
5701 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
5761 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
5821 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
5881 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
5941 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
6001 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
6061 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
6121 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
6181 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
6241 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
6301 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
6361 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
6421 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
6481 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
6541 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
6601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
6661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
6721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
6781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
6841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
6901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
6961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
7021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
7081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
7141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
7201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
7261 tgctcacatg t
//
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pAAVLINK-3'-ATRX-FLAG质粒载体应用举例
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