| 质粒名称: | pAAV-Syn-NES-FRCaMPi |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001087 |
| 产品用途: | Syn promoted nuclear excluded version of red calcium indicator, FRCaMPi for in vivo calcium imaging |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | Syn |
| 拷贝数: | 中拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | NES-FRCaMPi |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution
Zhou S., Zhu Q., Eom M., Fang S., Subach O.M., Ran C., Alvarado J.S., Sunkavalli P.S., Dong Y., Wang Y., Hu J., Zhang H., Wang Z., Sun X., Yang T., Mu Y., Yoon Y.G., Guo Z.V., Subach F.V., Piatkevich K.D.
PLOS Biol. 23, e3003048 (2025)
|
pAAV-Syn-NES-FRCaMPi
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-Syn-NES-FRCaMPi载体质粒基本信息
pAAV-Syn-NES-FRCaMPi质粒图谱载体图谱和pAAV-Syn-NES-FRCaMPi载体序列质粒序列多克隆位点信息
pAAV-Syn-NES-FRCaMPi质粒载体简介
pAAV-Syn-NES-FRCaMPi质粒序列载体序列
LOCUS pAAV-Syn-NES-FRCaMPi 5761 bp DNA circular SYN 14-SEP-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5761)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Aug 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5761
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 311..800
/label=Syn
promoter 349..796
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
primer_bind 734..755
/label=Syn-F
misc_feature 812..817
/label=Kozak sequence
CDS 818..2134
/codon_start=1
/label=NES-FRCaMPi
/translation="MLQLPPLERLTLSRSMVSKGEEDNMAIIKEFMRFKVHMEGSVNGH
EFEIEGEGEGRPYEAFQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFK
LSFPEGFRWERVMSFEDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGW
EASYEMTTRNLTDEQIAEFREAFSLLDRDQDGNITSNELGVVMRSLGLSPTAAELQDMI
NEVDADGNGTIDFTEFLTMMARKMKDTDDEEEVREAFKVFDKDGSGYITVEELTHVLTS
LGERLSREEVADVIREADSDGDGVINYEEFSRVISSKGGSGGSGSGSMRKPFRGAGNAV
RAFSTWKKLVPRVSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLPGA
YIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYK"
misc_feature 2159..2747
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2630..2641)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2765..2972
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 3024..3164
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3239..3694
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3976..4080
/gene="bla"
/label=AmpR promoter
CDS 4081..4941
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5112..5700
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
301 aattaattaa tggttaatta atctagactg cagagggccc tgcgtatgag tgcaagtggg
361 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
421 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
481 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
541 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
601 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
661 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
721 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
781 gtgcctgaga gcgcagtcga gaggatccta agccaccatg cttcaacttc ctcctcttga
841 acgtcttact ctttcgagat ctatggtgag caagggcgag gaggataaca tggccatcat
901 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
961 gatcgagggc gagggcgagg gccgccccta cgaggccttc cagaccgcta ggctgaaggt
1021 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
1081 ctccaaggcc tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc
1141 cgagggcttc aggtgggagc gcgtgatgag cttcgaggac ggcggcatta ttcacgttaa
1201 tcaggactcc tccctgcagg acggcgtatt catctacaag gtgaagctgc tcggcaccaa
1261 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cttcctacga
1321 gatgactacc cgtaacctta cagatgagca gattgcggag ttccgtgagg ccttttcgct
1381 gcttgatcgt gatcaggatg gaaatatcac gtccaatgaa ttgggtgtgg ttatgaggtc
1441 gttaggtcta tcgcctactg ccgccgaatt acaagatatg attaatgagg tcgatgccga
1501 tggtaatggc acaattgatt ttaccgaatt tttgacaatg atggcccgga aaatgaagga
1561 taccgacgac gaagaggaag ttcgcgaagc ctttaaagtc ttcgataaag atggaagtgg
1621 atacattaca gtcgaggagc tgactcatgt tcttacaagt ctcggtgaac gtttgtctcg
1681 agaagaagta gccgatgtga tacgtgaagc cgactccgat ggcgatggtg taatcaacta
1741 cgaagaattt tctcgtgtca tttcttccaa gggcggttca ggaggcagcg ggtcaggttc
1801 aatgaggaaa ccgttccgtg gcgcgggcaa cgctgtgcgt gctttcagca cttggaaaaa
1861 gctagtgccg cgggtttccg agtggatgta ccccgaggac ggcgccctga agagcgagat
1921 caggaagggg ctgaggctga aggacggcgg ccactacgcc gccgaggtca agaccaccta
1981 caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatcg agttggacat
2041 cttgtcccac aacgaggact acaccatcgt ggaacagtgc gaacgcgccg tgggccgcca
2101 ccccaccggt ggcacggacg cgctgtacaa gtaggaattc cgtacaaagc ttatcgataa
2161 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
2221 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
2281 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
2341 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
2401 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
2461 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
2521 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
2581 ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
2641 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
2701 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccggct
2761 cgagctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
2821 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
2881 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
2941 gattgggaag acaatagcag gcatgctggg gatgcggtct cgagctgatt ttgtaggtaa
3001 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
3061 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
3121 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
3181 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
3241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
3301 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
3361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
3421 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
3481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
3541 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
3601 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
3661 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
3721 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
3781 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
3841 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
3901 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
3961 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
4021 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
4081 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
4141 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
4201 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
4261 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
4321 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
4381 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
4441 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
4501 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
4561 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
4621 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
4681 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
4741 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
4801 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
4861 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
4921 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
4981 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
5041 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
5101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
5161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
5221 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
5281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
5341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
5401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
5461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
5521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
5581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
5641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
5701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
5761 t
//
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