| 质粒名称: | pBAD-HisD-Electra2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000249 |
| 产品用途: | Expresses Electra2 in E. coli |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: | araBAD |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Electra2 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
买家导航
pBAD-HisD-Electra2载体质粒基本信息
pBAD-HisD-Electra2质粒图谱载体图谱和pBAD-HisD-Electra2载体序列质粒序列多克隆位点信息
pBAD-HisD-Electra2质粒载体简介
pBAD-HisD-Electra2质粒序列载体序列
LOCUS pBAD-HisD-Electra2 4713 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4713)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4713
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 120..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
CDS 319..327
/codon_start=1
/label=Start
/translation="MGS"
primer_bind 327..376
/label=0509pBAD-El1-F
CDS 328..345
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 355..1068
/codon_start=1
/label=Electra2
/translation="MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFEQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGGLVYNVKVRGVNFHSKGPVMQKKTEGWEPFTEMMYPADGGLRGYTD
IALKVDGGGHLHANIVTTYRSKKTVGNIKMPGVHAVDYRLERIEESDNETYVVLREVAV
AKYSNLGGGMDELFK"
regulatory 355..358
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(1045..1092)
/label=0509pBAD-EL2-R
terminator 1284..1370
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1462..1489
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1508..1599
/gene="bla"
/label=AmpR promoter
CDS 1600..2460
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2631..3219
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 3405..3545
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3809..4687)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcct cgagatggtg
361 tctaagggcg aagagctgat cgaggaaaat atgcgtatga aggtggtcat ggaaggttcg
421 gtcaacggcc accaattcaa atgcacaggt gaaggagaag gcagaccgta cgagggagtg
481 caaaccatga ggatcaaagt catcgaggga ggacccctgc catttgcctt tgacattctt
541 gccacgtcgt tcctgttcgg cagcaagacc tttatcaagt acccggccga catccctgat
601 ttcttcgaac agtcctttcc tgagggcttt acttgggaaa gagttacgag atacgaagat
661 ggtggagtag tcaccgtcac gcaggacacc agccttgagg atggtgggct cgtctacaac
721 gtcaaggtca gaggggtaaa ctttcactcc aagggtcccg tgatgcagaa gaagaccgag
781 ggttgggagc ctttcacaga gatgatgtat ccagcagatg gtggtctgag aggatatacc
841 gacatcgcac tgaaagttga tggtggtggc catctgcacg ccaacatcgt gacaacttac
901 aggtcaaaaa agaccgtcgg gaacatcaag atgcccggtg tccatgccgt tgattaccgc
961 ctggaaagga tcgaagagag tgacaatgaa acctacgtag tgctaagaga agtggcagtt
1021 gccaaataca gcaaccttgg tggtggcatg gacgagctgt tcaagtaaga attcgaagct
1081 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
1141 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
1201 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
1261 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
1321 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
1381 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
1441 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
1501 aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
1561 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
1621 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
1681 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
1741 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
1801 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
1861 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
1921 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
1981 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
2041 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
2101 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
2161 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
2221 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
2281 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
2341 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
2401 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
2461 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
2521 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
2581 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
2641 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
2701 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
2761 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
2821 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
2881 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
2941 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
3001 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
3061 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
3121 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
3181 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
3241 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
3301 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
3361 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
3421 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
3481 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
3541 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
3601 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
3661 cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg
3721 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
3781 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
3841 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
3901 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
3961 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
4021 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
4081 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
4141 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
4201 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
4261 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
4321 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
4381 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
4441 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
4501 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
4561 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
4621 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
4681 cagccatact tttcatactc ccgccattca gag
//
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