pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD2-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD2-FLAG                6710 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3423
                     /codon_start=1
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /product="chromodomain-helicase-DNA-binding protein 
                     2isoform 1"
                     /EC_number="3.6.4.12"
                     /label=CHD2
                     /note="isoform 1 is encoded by transcript variant 1;CHD-2; 
                     ATP-dependent helicase CHD2"
                     /db_xref="GI:118421089"
                     /db_xref="CCDS:CCDS10374.2"
                     /db_xref="GeneID:1106"
                     /db_xref="HGNC:HGNC:1917"
                     /db_xref="MIM:602119"
                     /protein_id="NP_001262.3"
                     /translation="KRRGPTIKISGVQVNVKSIIQHEEEFEMLHKSIPVDPEEKKKYCL
                     TCRVKAAHFDVEWGVEDDSRLLLGIYEHGYGNWELIKTDPELKLTDKILPVETDKKPQG
                     KQLQTRADYLLKLLRKGLEKKGAVTGGEEAKLKKRKPRVKKENKVPRLKEEHGIELSSP
                     RHSDNPSEEGEVKDDGLEKSPMKKKQKKKENKENKEKQMSSRKDKEGDKERKKSKDKKE
                     KPKSGDAKSSSKSKRSQGPVHITAGSEPVPIGEDEDDDLDQETFSICKERMRPVKKALK
                     QLDKPDKGLNVQEQLEHTRNCLLKIGDRIAECLKAYSDQEHIKLWRRNLWIFVSKFTEF
                     DARKLHKLYKMAHKKRSQEEEEQKKKDDVTGGKKPFRPEASGSSRDSLISQSHTSHNLH
                     PQKPHLPASHGPQMHGHPRDNYNHPNKRHFSNADRGDWQRERKFNYGGGNNNPPWGSDR
                     HHQYEQHWYKDHHYGDRRHMDAHRSGSYRPNNMSRKRPYDQYSSDRDHRGHRDYYDRHH
                     HDSKRRRSDEFRPQNYHQQDFRRMSDHRPAMGYHGQGPSDHYRSFHTDKLGEYKQPLPP
                     LHPAVSDPRSPPSQKSPHDSKSPLDHRSPLERSLEQKNNPDYNWNVRKT"
     misc_feature    2527..2532
                     /label=SDS
     CDS             3430..3495
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3499..3930
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3643..3648
                     /label=SDS
     repeat_region   3973..4113
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3973..4102
                     /label=AAV2 ITR
     repeat_region   3974..4114
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4188..4643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4925..5029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5030..5890
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6061..6649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggaa aaggagaggt
     1561 ccaacaatca agatatccgg agttcaggtt aatgtgaaat ccattatcca acatgaagag
     1621 gagtttgaga tgctgcataa atctatccct gtggaccctg aagaaaaaaa aaaatactgc
     1681 ttaacctgtc gtgtcaaagc tgcacatttt gatgtagagt ggggggtgga agatgattct
     1741 cgcctgttgc tggggattta tgaacatggc tatggaaact gggagttaat taaaacagac
     1801 ccagagctta aattaactga caaaattctg ccggtggaga cagataaaaa gcctcagggg
     1861 aagcagctac agacccgagc ggattacttg ttgaagctgc tcagaaaggg tctggagaag
     1921 aagggggctg tgacaggtgg ggaagaggcc aaattaaaga agcggaagcc tcgggtaaag
     1981 aaggaaaaca aagtgcccag gctgaaagag gagcatggaa ttgagctttc atctcctagg
     2041 cattcagata atccatcaga agagggagaa gtgaaagatg atggcttgga aaaaagtcca
     2101 atgaaaaaaa aacagaagaa gaaagagaac aaggagaaca aggagaaaca aatgagttct
     2161 aggaaagaca aagaagggga caaggaaaga aagaagtcaa aagataagaa agagaagcct
     2221 aaaagtggtg atgccaaatc ttcgagtaaa tcaaagcgat ctcagggtcc tgtccatatt
     2281 acagcaggaa gtgaacctgt ccccattgga gaggatgagg atgatgatct ggaccaggag
     2341 acattcagca tatgtaagga gaggatgagg cccgtgaaaa aggcactgaa acagctcgac
     2401 aaacctgaca aggggctcaa cgtgcaagaa cagctggaac acacccggaa ctgcctgctg
     2461 aaaatcggag accggatagc cgagtgcctt aaagcctact cagatcagga gcacatcaaa
     2521 ctctggagga ggaacctatg gatttttgtt tccaagttta cagaatttga tgctcgaaaa
     2581 ctgcataagt tatacaagat ggctcataag aaaaggtctc aagaagaaga ggagcaaaag
     2641 aagaaagacg acgtgactgg gggtaagaaa ccatttcgtc cagaggcctc aggctccagc
     2701 cgggactctc tgatatctca gtcccatacc tcacacaacc ttcaccctca gaagcctcat
     2761 ttgcctgcct cccatggccc acagatgcat ggacacccaa gagataacta caatcacccc
     2821 aacaagagac acttcagtaa tgcagatcga ggagactggc agagggaaag aaagttcaac
     2881 tatggtggtg gcaacaacaa tccaccatgg ggaagcgaca ggcaccatca gtatgagcag
     2941 cactggtaca aggaccacca ttatggggac cggcgacata tggatgccca ccgttccgga
     3001 agctatcgac ccaacaacat gtccagaaag aggccttatg accagtacag cagtgaccga
     3061 gaccaccggg gacacagaga ttattatgac aggcaccatc atgactccaa gcggaggaga
     3121 tccgatgaat ttaggcctca aaattaccac cagcaggatt tccgacgaat gtctgatcac
     3181 cgccccgcta tgggctacca tggccaggga ccctcagacc attaccgctc tttccacaca
     3241 gataaactgg gggaatataa acagcctcta cccccattgc accctgcagt ctcagatcct
     3301 cgctcacccc cttctcagaa atctcctcac gattccaagt cacccctgga tcataggtct
     3361 cctttggaga gatcactaga acagaaaaac aacccagatt ataactggaa tgttcggaaa
     3421 acatgtacag actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     3481 gatgacgatg acaagtaact cgagataatc aacctctgga ttacaaaatt tgtgaaagat
     3541 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3601 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3661 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3721 ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg
     3781 ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3841 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3901 gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg cacgtgcgga
     3961 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4021 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4081 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     4141 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     4201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4261 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4381 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4501 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     4561 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4621 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     4681 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4741 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4801 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4861 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4921 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4981 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5041 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5101 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5161 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5221 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5281 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5341 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5401 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5461 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5521 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5581 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5641 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5701 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5761 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5821 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5881 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5941 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6001 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6061 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6121 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6181 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6241 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6301 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6361 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6421 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6481 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6541 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6601 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6661 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//