| 质粒名称: | pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001190 |
| 产品用途: | in vivo |
| 存储实验室: | Yuxuan Guo Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒表达 |
| 启动子: |
hTNNT2
|
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
|
插入基因信息 |
|
| 插入基因名称: | YAP5SA |
| 物种来源: | MUS |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Neural Organoids Protect Engineered Heart Tissues From Glucolipotoxicity by Transferring Versican in a Co-Culture System.
Bai B., Li J., Wang Z., Yang Y., He J., Chen G., Zhang Y., Qi Y., Wan Z., Cai L., Wang R., Wang K., Zhao D., Zhang J., Huang W., Xu X.R., Sun M., Han X., Liu Y., Zhang D., Zhu W., Liu J., Guo Y.
Cell Prolif. 0, e70070 (2025)
|
买家导航
pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122载体质粒基本信息
pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122质粒图谱载体图谱和pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122载体序列质粒序列多克隆位点信息
pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122质粒载体简介
pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122质粒序列载体序列
LOCUS pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122 bp ds-DNA circular 21-JUN-2024
DEFINITION .
COMMENT ^^
FEATURES Location/Qualifiers
misc_feature 1..130
/label="5' ITR"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
misc_feature 29..34
/label="XmaI"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 40..45
/label="XmaI"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
misc_feature 176..197
/label="overlap"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
misc_feature 178..197
/label="nhe1cut"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 198..218
/label="overlap"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 198..741
/label="hTNT promoter"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 733..756
/label="NOT1 cut"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
primer 737..777
/label="tnt4-zn-clone-F"
/note="sequence: GGCAGagctCCTCGAGAAGcACTGACCAACAGCGTGAAGAC"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 757..777
/label="overlap"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 757..795
/label="upstream"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
exon 757..795
/label="Zfr-201 Exon 19"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
gene 757..1989
/label="Zfr minigene(1233BP)"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
primer 769..792
/label="Zfr-rtpcr-F"
/note="sequence: CGTGAAGACATTACATCCAGTGCA"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 841..930
/label="I1D1"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 841..1200
/label="to delete"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 931..1020
/label="I1D2"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 1021..1110
/label="I1D3"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 1111..1200
/label="I1D4"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 1261..1264
/label="bp"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 1296..1379
/label="PSEX"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
CDS 1299..1379
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="KGLHIKNIV*TL*GQRSVLISPD*KL*"
primer 1345..1369
/label="Zfr-qpcr-F"
/note="sequence: GAAGTGTGCTCATTTCTCCCGATTA"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
CDS 1376..1379
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="M"
misc_feature 1380..1948
/label="keep"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
misc_feature 1433..1522
/label="I2D1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 1433..1882
/label="to delete"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 1523..1612
/label="I2D2"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 1613..1702
/label="I2D3"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 1703..1792
/label="I2D4"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 1793..1882
/label="I2D5"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 1933..1937
/label="bp"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 1949..1989
/label="downstream"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
exon 1949..1989
/label="Zfr-201 Exon 20"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
CDS 1951..1989
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="CIETPCIPSDTQS"
misc_feature 1966..1989
/label="overlap"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
misc_feature 1990..2046
/label="P2A"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
CDS 1990..2046
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="ATNFSLLKQAGDVEENPGP"
primer complement(2003..2025)
/label="P2A-R1"
/note="sequence: gtctccagcctgTttTagTaggc"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
primer complement(2005..2029)
/label="P2A-R2"
/note="sequence: cAacgtctccagcctgTttTagTag"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 2007..2028
/label="non-AUG start codons removed"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer complement(2011..2031)
/label="P2A-R5"
/note="sequence: ctcAacgtctccagcctgTtt"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
primer complement(2017..2037)
/label="P2A-R3"
/note="sequence: gttctcctcAacgtctccagc"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
primer complement(2025..2044)
/label="P2A-R4"
/note="sequence: gtccagggttctcctcAacg"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
CDS 2047..2070
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="YPYDVPDY"
misc_feature 2047..2073
/label="HA tag"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 2071..2089
/label="NOT1 cut"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
CDS 2071..3543
/label="Translation 2071-3543"
/translation="ADPGQQPPPQPAPQGQGQPPSQPPQGQGPPSGPGQPAPAATQAAPQAPPAGHQIVHVRGDAETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQAATDAGTAGALTPQHVRAHAAPAALQLGAVSPGTLTPTGVVSGPAATPTAQHLRQAAFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDEATDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWLYL*"
exon 2073..2391
/label="YAP1"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
CDS 2073..3533
/label="YAP1"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
misc_feature 2251..2253
/label="YAP1"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
misc_feature 2257..2259
/label="YAP1"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
PCR_primer 2266..2292
/label="hYAP-seq-F1"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 2383..2385
/label="YAP1"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
exon 2392..2642
/label="YAP1"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 2395..2397
/label="YAP1"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
misc_feature 2398..2400
/label="YAP1"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 2425..2427
/label="YAP1"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 2449..2451
/label="YAP1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 2449..2451
/label="Zhiqiang's S127A mutation"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
misc_feature 2452..2454
/label="YAP1"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
misc_feature 2461..2463
/label="YAP1"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 2482..2484
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 2530..2532
/label="YAP1"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 2557..2559
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 2560..2562
/label="YAP1"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
exon 2643..2758
/label="YAP1"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
exon 2759..2872
/label="YAP1"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
PCR_primer 2844..2873
/label="hYAP-Seq-F2"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
exon 2873..3054
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 2890..2892
/label="YAP1"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 2935..2937
/label="YAP1"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 2941..3533
/label="YAP1"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 3053..3058
/label="skip one exon here in Zhiqiang's plasmid"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
exon 3055..3055
/label="YAP1"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
exon 3056..3185
/label="YAP1"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 3121..3123
/label="YAP1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 3133..3135
/label="YAP1"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 3166..3168
/label="YAP1"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
exon 3186..3298
/label="YAP1"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
misc_feature 3211..3213
/label="YAP1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 3220..3222
/label="YAP1"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 3229..3231
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 3241..3243
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 3256..3258
/label="YAP1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
exon 3299..3533
/label="YAP1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
PCR_primer 3370..3394
/label="hYAP-Seq-F3"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 3505..3533
/label="overlap"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
misc_feature 3528..3547
/label="kpn1-CUT"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
primer 3528..3568
/label="mir122-clone-F"
/note="sequence: atggttGTACCTCTAGGTACgatggggactgaaagcttcgg"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
misc_feature 3548..3568
/label="ol"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 3560..3560
/label="overlap"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 3561..3565
/label="HindIII"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 3566..3586
/label="PCR-mi122-F"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
misc_feature 3566..3652
/label="合成"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
source 3566..3652
/label="source"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
/note="organism: synthetic DNA construct mol_type: other DNA"
misc_feature 3570..3591
/label="miR122"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 3598..3619
/label="miR122"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 3626..3647
/label="miR122"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 3637..3652
/label="PCR-mi122-R"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 3648..3668
/label="ol"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
primer complement(3648..3688)
/label="mir122-clone-R"
/note="sequence: CGTCCTCGAGGCCGCCCGGGcggtatcgataagctTcggcg"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
misc_feature 3653..3657
/label="HindIII"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 3669..3688
/label="sal1-cut"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
terminator 3712..3838
/label="PolyA"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
primer complement(3743..3762)
/label="polyA-R"
/note="sequence: CTCAAGGGGCTTCATGATGT"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
misc_feature 3927..4056
/label="3' ITR"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
misc_feature 4012..4017
/label="XmaI"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 4023..4028
/label="XmaI"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
rep_origin 4233..4688
/label="f1 origin"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 4714..4818
/label="Amp Promoter"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 4819..5676
/label="Amp resistent"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
rep_origin 5850..6438
/label="origin"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
ORIGIN
1 CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT
61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG
181 ATCCTCTAGA ACTATAGCTC AGTCCATTAG GAGCCAGTAG CCTGGAAGAT GTCTTTACCC
241 CCAGCATCAG TTCAAGTGGA GCAGCACATA ACTCTTGCCC TCTGCCTTCC AAGATTCTGG
301 TGCTGAGACT TATGGAGTGT CTTGGAGGTT GCCTTCTGCC CCCCAACCCT GCTCCCAGCT
361 GGCCCTCCCA GGCCTGGGTT GCTGGCCTCT GCTTTATCAG GATTCTCAAG AGGGACAGCT
421 GGTTTATGTT GCATGACTGT TCCCTGCATA TCTGCTCTGG TTTTAAATAG CTTATCTGAG
481 CAGCTGGAGG ACCACATGGG CTTATATGGC GTGGGGTACA TGTTCCTGTA GCCTTGTCCC
541 TGGCACCTGC CAAAATAGCA GCCAACAccc cccaccccca ccgccatccc cctgccccac
601 ccgtccccTG TCGCACATTC CTCCCTCCGC AGGGCTGGCT CACCAGGCCC CAGCCCACAT
661 GCCTGCTTAA AGCCCTCTCC ATCCTCTGCC TCACCCAGTC CCCGCTGAGA CTGAGCAGAC
721 GCCTCCAGGA TCTGTCGGCA GagctCCTCG AGAAGcACTG ACCAACAGCG TGAAGACATT
781 ACATCCAGTG CACAGGTAAT TGAATTCTTG TTGGATTACT GTCTCTAAGG CCTGGGAGTT
841 TTATAATTGT TTCTTTATGT GTAATAATAA CCGTACTAGT CATTGTGATA CTTATTTGGG
901 AAGTAAGCCT TTAAAGAGGT TATTAAAAAG ATTTACTGAT TCCCACACCG GAAGTTGCTC
961 TTCTAGCCGT GAAAGAAGGA CGGCGTGGTC TTCAGTTTGC TATGTCCACT AACAATATGT
1021 CCGACCCACG AAGGCCCACC AAAGTTATAC TTGTGGCACA TATAAACCTT TGGAATCAAG
1081 GGACCTGATA CTGGATTATT AAATATTTAA GAGAATTTAT ATTTAATAAA CTTGATACTT
1141 CAGTGAGAAA GGACAAATCA TCAGTAGAAT GGTTTTGAGA TAGCTGATGA TTCTATTAAA
1201 AGTAGATATG TGTGTGGGTT AAATGACTTA AGCATACATC AAAAGACTGA GAAAGGCTCT
1261 TAACATTAAA AGATGGGCCt ttttttttCT TTTAGGTTAA AGGATTACAT ATTAAAAATA
1321 TTGTATAGAC TCTTTAAGGC CAAAGAAGTG TGCTCATTTC TCCCGATTAA AAGTTATGAG
1381 TAAGAATTGG GTATTTTGCC TTTTGGGGGT GGGAGAtttg aaacagggtt tctccttaca
1441 gctctggctt tcctggagca gtgaattgag cctggctcct ctgtaggagt aacgagttgc
1501 tttaaccact gacatctttc caccaccacc accaccCCTT ATGAATAGAC TAAATTTATT
1561 TAAAGATTGA TATTTTCTAG CTTAATTATT TGGAAAGCAA ATAAGCACAC AGAAAAGCCT
1621 CCTCCCCaaa aaaatataaa taaaaaaGTC TATCAGGACA TAGTATATGT CCCTTCTGAT
1681 TAATCATTGG TGGTGAAACA ACCCATTCAG GGTGTCTCTT CCATCACAGT GGAttttttt
1741 CATATTGTTA CATGGAGAAG TTAACTTAGT AAGAATTGAT GTGCTTCTGT GCCAGTGAGA
1801 TAGCTCTGCA GGTAAAAGGG AGACTGACCC CCAACTTGTA CTCTGGCCGT GGCAGTCATG
1861 TGCACACAAG TAAACTGAAT AAATATAAAG AGCAGATTTT AGAGTGTTTT GGCTTTAAAT
1921 TAATTCTATA TTCTAACTCT ATTTGCAGTT TGCATTGAGA CTCCTTGCAT TCCGTCAGAT
1981 ACACAAAGTg ctactaactt cagcctActA aaAcaggctg gagacgtTga ggagaaccct
2041 ggacctTATC CATAcGACGT TCCAGATTAC GCagatcccg ggcagcagcc gccgcctcaa
2101 ccggcccccc agggccaagg gcagccgcct tcgcagcccc cgcaggggca gggcccgccg
2161 tccggacccg ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc accccccgcc
2221 gggcatcaga tcgtgcacgt ccgcggggac gcggagaccg acctggaggc gctcttcaac
2281 gccgtcatga accccaagac ggccaacgtg ccccagaccg tgcccatgag gctccggaag
2341 ctgcccgact ccttcttcaa gccgccggag cccaaatccc actcccgaca ggccgctact
2401 gatgcaggca ctgcaggagc cctgactcca cagcatgttc gagctcatgc cgctccagct
2461 gctctgcagt tgggagctgt ttctcctggg acactgaccc ccactggagt agtctctggc
2521 ccagcagcta cacccacagc tcagcatctt cgacaggctg cttttgagat acctgatgat
2581 gtacctctgc cagcaggttg ggagatggca aagacatctt ctggtcagag atacttctta
2641 aatcacatcg atcagacaac aacatggcag gaccccagga aggccatgct gtcccagatg
2701 aacgtcacag cccccaccag tccaccagtg cagcagaata tgatgaactc ggcttcaggt
2761 cctcttcctg atggatggga acaagccatg actcaggatg gagaaattta ctatataaac
2821 cataagaaca agaccacctc ttggctagac ccaaggcttg accctcgttt tgccatgaac
2881 cagagaatca gtcagagtgc tccagtgaaa cagccaccac ccctggctcc ccagagccca
2941 cagggaggcg tcatgggtgg cagcaactcc aaccagcagc aacagatgcg actgcagcaa
3001 ctgcagatgg agaaggagag gctgcggctg aaacagcaag aactgcttcg gcaggAgtta
3061 gccctgcgta gccagttacc aacactggag caggatggtg ggactcaaaa tccagtgtct
3121 tctcccggga tgtctcagga attgagaaca atgacgacca atagctcaga tcctttcctt
3181 aacagtggca cctatcactc tcgagatgag gctacagaca gtggactaag catgagcagc
3241 tacagtgtcc ctcgaacccc agatgacttc ctgaacagtg tggatgagat ggatacaggt
3301 gatactatca accaaagcac cctgccctca cagcagaacc gtttcccaga ctaccttgaa
3361 gccattcctg ggacaaatgt ggaccttgga acactggaag gagatggaat gaacatagaa
3421 ggagaggagc tgatgccaag tctgcaggaa gctttgagtt ctgacatcct taatgacatg
3481 gagtctgttt tggctgccac caagctagat aaagaaagct ttcttacatg gttGTACCTC
3541 TAGGTACgat ggggactgaa agcttcggtc aaacaccatt gtcacactcc atactatcaa
3601 acaccattgt cacactccag atcaacaaac accattgtca cactccacgc cgAagcttat
3661 cgataccgCC CGGGCGGCCT CGAGGACGGG GTGAACTACG CCTGAGGATC CGATCTTTTT
3721 CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA
3781 TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA
3841 AGCAATTCGT TGATCTGAAT TTCGACCACC CATAATACCC ATTACCCTGG TAGATAAGTA
3901 GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC
3961 TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT
4021 TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAACCTA ATTCACTGGC
4081 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
4141 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC
4201 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAAGCGC
4261 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC
4321 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT
4381 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA
4441 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC
4501 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT
4561 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG
4621 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT
4681 TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC
4741 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA
4801 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT
4861 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT
4921 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC
4981 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA
5041 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC
5101 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC
5161 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC
5221 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG
5281 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC
5341 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC
5401 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT
5461 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA
5521 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC
5581 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG
5641 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA
5701 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC
5761 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA
5821 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC
5881 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA
5941 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT
6001 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC
6061 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG
6121 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG
6181 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG
6241 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC
6301 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT
6361 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
6421 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
6481 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT
6541 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA
6601 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG
6661 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC
6721 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
6781 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC
6841 CATGATTACG CCAGATTTAA TTAAGGCCTT AATTAGG
//
pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122载体图谱质粒图谱pdf版和pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122载体序列质粒序列等相关资料下载
pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122质粒载体应用举例
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