pAtCas9-dead_2

LOCUS       pAtCas9-dead_2               13999 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant 
            Selection: 2x35S:npt II.
ACCESSION   .
VERSION     .
KEYWORDS    pAtCas9-dead_2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13999)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 13999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13999
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3379)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    3570..3591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3606..3636
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3649..3671
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3668..3684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3668..3684
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4214..4559
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4593..8696
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8709..8729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9010..9027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9010..9026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9019..9041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9229..9253
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10554..11183
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11612..12685
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12751..12945
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13181..13203)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13289..13429
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13344..13363
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13444..13461)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13615..204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13695..13714)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcggtacc cggggatcgg
     3721 cgcgccagat ttgccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg
     3781 cttacgcagc aggtatcatc aagacgatct acccgagcaa taatctccag gaaatcaaat
     3841 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
     3901 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
     3961 tgcttcacaa accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg
     4021 aatcaaaggc catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga
     4081 ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca
     4141 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
     4201 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
     4261 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
     4321 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
     4381 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     4441 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     4501 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac
     4561 gggggactcc tgcaggtaga tcgctcgtcg acatggataa gaagtactct atcggactcg
     4621 ctatcggaac taactctgtg ggatgggctg tgatcaccga tgagtacaag gtgccatcta
     4681 agaagttcaa ggttctcgga aacaccgata ggcactctat caagaaaaac cttatcggtg
     4741 ctctcctctt cgattctggt gaaactgctg aggctaccag actcaagaga accgctagaa
     4801 gaaggtacac cagaagaaag aacaggatct gctacctcca agagatcttc tctaacgaga
     4861 tggctaaagt ggatgattca ttcttccaca ggctcgaaga gtcattcctc gtggaagaag
     4921 ataagaagca cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg gcataccacg
     4981 agaagtaccc tactatctac cacctcagaa agaagctcgt tgattctact gataaggctg
     5041 atctcaggct catctacctc gctctcgctc acatgatcaa gttcagagga cacttcctca
     5101 tcgagggtga tctcaaccct gataactctg atgtggataa gttgttcatc cagctcgtgc
     5161 agacctacaa ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg
     5221 ctatcctctc tgctaggctc tctaagtcaa gaaggcttga gaacctcatt gctcagctcc
     5281 ctggtgagaa gaagaacgga cttttcggaa acttgatcgc tctctctctc ggactcaccc
     5341 ctaacttcaa gtctaacttc gatctcgctg aggatgcaaa gctccagctc tcaaaggata
     5401 cctacgatga tgatctcgat aacctcctcg ctcagatcgg agatcagtac gctgatttgt
     5461 tcctcgctgc taagaacctc tctgatgcta tcctcctcag tgatatcctc agagtgaaca
     5521 ccgagatcac caaggctcca ctctcagctt ctatgatcaa gagatacgat gagcaccacc
     5581 aggatctcac acttctcaag gctcttgtta gacagcagct cccagagaag tacaaagaga
     5641 ttttcttcga tcagtctaag aacggatacg ctggttacat cgatggtggt gcatctcaag
     5701 aagagttcta caagttcatc aagcctatcc tcgagaagat ggatggaacc gaggaactcc
     5761 tcgtgaagct caatagagag gatcttctca gaaagcagag gaccttcgat aacggatcta
     5821 tccctcatca gatccacctc ggagagttgc acgctatcct tagaaggcaa gaggatttct
     5881 acccattcct caaggataac agggaaaaga ttgagaagat tctcaccttc agaatccctt
     5941 actacgtggg acctctcgct agaggaaact caagattcgc ttggatgacc agaaagtctg
     6001 aggaaaccat caccccttgg aacttcgaag aggtggtgga taagggtgct agtgctcagt
     6061 ctttcatcga gaggatgacc aacttcgata agaaccttcc aaacgagaag gtgctcccta
     6121 agcactcttt gctctacgag tacttcaccg tgtacaacga gttgaccaag gttaagtacg
     6181 tgaccgaggg aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg
     6241 atctcttgtt caagaccaac agaaaggtga ccgtgaagca gctcaaagag gattacttca
     6301 agaaaatcga gtgcttcgat tcagttgaga tttctggtgt tgaggatagg ttcaacgcat
     6361 ctctcggaac ctaccacgat ctcctcaaga tcattaagga taaggatttc ttggataacg
     6421 aggaaaacga ggatatcttg gaggatatcg ttcttaccct caccctcttt gaagatagag
     6481 agatgattga agaaaggctc aagacctacg ctcatctctt cgatgataag gtgatgaagc
     6541 agttgaagag aagaagatac actggttggg gaaggctctc aagaaagctc attaacggaa
     6601 tcagggataa gcagtctgga aagacaatcc ttgatttcct caagtctgat ggattcgcta
     6661 acagaaactt catgcagctc atccacgatg attctctcac ctttaaagag gatatccaga
     6721 aggctcaggt ttcaggacag ggtgatagtc tccatgagca tatcgctaac ctcgctggat
     6781 ctcctgcaat caagaaggga atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg
     6841 tgatgggaag gcataagcct gagaacatcg tgatcgaaat ggctagagag aaccagacca
     6901 ctcagaaggg acagaagaac tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag
     6961 agcttggatc tcagatcctc aaagagcacc ctgttgagaa cactcagctc cagaatgaga
     7021 agctctacct ctactacctc cagaacggaa gggatatgta tgtggatcaa gagttggata
     7081 tcaacaggct ctctgattac gatgttgatg ctatcgtgcc acagtcattc ttgaaggatg
     7141 attctatcga taacaaggtg ctcaccaggt ctgataagaa caggggtaag agtgataacg
     7201 tgccaagtga agaggttgtg aagaaaatga agaactattg gaggcagctc ctcaacgcta
     7261 agctcatcac tcagagaaag ttcgataact tgactaaggc tgagagggga ggactctctg
     7321 aattggataa ggcaggattc atcaagaggc agcttgtgga aaccaggcag atcactaagc
     7381 acgttgcaca gatcctcgat tctaggatga acaccaagta cgatgagaac gataagttga
     7441 tcagggaagt gaaggttatc accctcaagt caaagctcgt gtctgatttc agaaaggatt
     7501 tccaattcta caaggtgagg gaaatcaaca actaccacca cgctcacgat gcttacctta
     7561 acgctgttgt tggaaccgct ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt
     7621 acggtgatta caaggtgtac gatgtgagga agatgatcgc taagtctgag caagagatcg
     7681 gaaaggctac cgctaagtat ttcttctact ctaacatcat gaatttcttc aagaccgaga
     7741 ttaccctcgc taacggtgag atcagaaaga ggccactcat cgagacaaac ggtgaaacag
     7801 gtgagatcgt gtgggataag ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc
     7861 cacaggtgaa catcgttaag aaaaccgagg tgcagaccgg tggattctct aaagagtcta
     7921 tcctccctaa gaggaactct gataagctca ttgctaggaa gaaggattgg gaccctaaga
     7981 aatacggtgg tttcgattct cctaccgtgg cttactctgt tctcgttgtg gctaaggttg
     8041 agaagggaaa gagtaagaag ctcaagtctg ttaaggaact tctcggaatc actatcatgg
     8101 aaaggtcatc tttcgagaag aacccaatcg atttcctcga ggctaaggga tacaaagagg
     8161 ttaagaagga tctcatcatc aagctcccaa agtactcact cttcgaactc gagaacggta
     8221 gaaagaggat gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt gctctcccat
     8281 ctaagtacgt taactttctt tacctcgctt ctcactacga gaagttgaag ggatctccag
     8341 aagataacga gcagaagcaa cttttcgttg agcagcacaa gcactacttg gatgagatca
     8401 tcgagcagat ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg
     8461 tgttgtctgc ttacaacaag cacagagata agcctatcag ggaacaggca gagaacatca
     8521 tccatctctt cacccttacc aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa
     8581 ccatcgatag gaagagatac acctctacca aagaagtgct cgatgctacc ctcatccatc
     8641 agtctatcac tggactctac gagactagga tcgatctctc acagctcggt ggtgattcaa
     8701 gggctgatcc taagaagaag aggaaggttt gactcgagat atgaagatga agatgaaata
     8761 tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg
     8821 tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatcacat tataatgaat
     8881 aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctg cagcatataa
     8941 ctactgtatg tgctatggta tggactatgg aatatgatta aagataagga gctccggtga
     9001 cggactggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     9061 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     9121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     9181 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     9241 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     9301 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     9361 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     9421 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     9481 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     9541 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     9601 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     9661 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     9721 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     9781 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     9841 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     9901 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     9961 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
    10021 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
    10081 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
    10141 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
    10201 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
    10261 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
    10321 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
    10381 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
    10441 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
    10501 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
    10561 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
    10621 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
    10681 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
    10741 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
    10801 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
    10861 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
    10921 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
    10981 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
    11041 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
    11101 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
    11161 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
    11221 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
    11281 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
    11341 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
    11401 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
    11461 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
    11521 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
    11581 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
    11641 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
    11701 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
    11761 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
    11821 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
    11881 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
    11941 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
    12001 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
    12061 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
    12121 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
    12181 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
    12241 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
    12301 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
    12361 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
    12421 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
    12481 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
    12541 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
    12601 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
    12661 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
    12721 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
    12781 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
    12841 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
    12901 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
    12961 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
    13021 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
    13081 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
    13141 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    13201 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    13261 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
    13321 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    13381 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
    13441 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    13501 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    13561 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    13621 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    13681 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    13741 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    13801 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    13861 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    13921 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    13981 gattagcaga gcgaggtat
//