pCytERM-mScarlet-I3

LOCUS       pCytERM-mScarlet-I3             4782 bp DNA     circular SYN 22-OCT-2024
DEFINITION  TSN20241013-0571-00010_751_20241014-BAN4_B03_B03_i20.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    88..135
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      464..1052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        1227..1530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1531..1734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             1786..1872
                     /codon_start=1
                     /label=cytochrome p450
                     /translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
     regulatory      1918..1927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1924..2610
                     /codon_start=1
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     polyA_signal    2736..2857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2864..3319)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3346..3450
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3452..3809
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3660..3795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3844..4638
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
       61 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
      121 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
      181 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
      241 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
      301 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
      361 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
      421 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      481 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      541 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      601 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      661 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      721 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      781 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      841 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      901 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      961 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1021 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1081 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1141 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     1201 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1261 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1321 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1381 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1441 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1501 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     1561 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     1621 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     1681 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     1741 gtgaaccgtc agatccgcta gcgctaccgg actcagatct ccaccatgga tcctgtggtg
     1801 gtgctggggc tctgtctctc ctgtttgctt ctcctttcac tctggaaaca gagctatggg
     1861 ggagggaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc
     1921 accatggata gcaccgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
     1981 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2041 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
     2101 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
     2161 cccgactact ggaagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgatcttc
     2221 gaggacggcg gcaccgtgtc cgtgacccag gacacctccc tggaggacgg caccctgatc
     2281 tacaaggtga agctccgcgg cggcaacttc cctcctgacg gccccgtaat gcagaagcgg
     2341 acaatgggct gggaagcatc caccgagcgg ttgtaccccg aggacgtcgt gctgaagggc
     2401 gacattaaga tggccctgcg cctgaaggac ggcggccggt acctggcgga cttcaagacc
     2461 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct tcaacatcga ccgcaagttg
     2521 gacatcacat cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgtggcc
     2581 cgccactcca ccggcggctc cggtggctcc taaagcggcc gcgactctag atcataatca
     2641 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2701 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2761 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2821 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     2881 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     2941 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     3001 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3061 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3121 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     3181 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     3241 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     3301 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3361 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3421 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     3481 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3541 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3601 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     3661 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3721 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3781 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     3841 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     3901 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     3961 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4021 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     4081 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     4141 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     4201 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     4261 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     4321 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     4381 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     4441 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     4501 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     4561 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     4621 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     4681 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4741 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg gg
//