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pcDNA3.1+/C-DYK载体质粒基本信息
pcDNA3.1+/C-DYK质粒图谱载体图谱和pcDNA3.1+/C-DYK载体序列质粒序列多克隆位点信息
pcDNA3.1+/C-DYK质粒载体简介
pcDNA3.1+/C-DYK质粒序列载体序列
LOCUS pcDNA3.1+ C-DYK 5438 bp ds-DNA circular SYN 21-MAY-2015
DEFINITION Mammalian expression vector with a CMV promoter for expressing
proteins with a C-terminal FLAG(R) (DYK) tag.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1+ C-DYK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5438)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5438
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 895..985
/note="MCS"
/note="multiple cloning site"
CDS 986..1009
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG (DYK) tag"
/translation="DYKDDDDK"
polyA_signal 1035..1259
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1305..1733
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1747..2076
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1927..2062
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2143..2937
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3111..3232
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3683..4271)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4442..5302)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5303..5407)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccgaattc tgcagatatc cagcacagtg
961 gcggccgctc gagtctagag ggcccgatta caaggatgac gacgataagt gataaacccg
1021 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
1081 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
1141 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
1201 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
1261 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
1321 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
1381 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
1441 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
1501 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
1561 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
1621 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
1681 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
1741 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
1801 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
1861 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
1921 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
1981 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
2041 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
2101 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
2161 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
2221 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
2281 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
2341 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
2401 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
2461 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
2521 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
2581 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
2641 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
2701 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
2761 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
2821 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
2881 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
2941 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
3001 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
3061 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
3121 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
3181 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
3241 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
3301 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
3361 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
3421 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
3481 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
3541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
3601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
3661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
3721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
3781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
3841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
3901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
3961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
4021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
4081 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
4141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
4201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
4261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
4321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
4381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
4441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
4501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
4561 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
4621 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
4681 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
4741 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
4801 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
4861 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
4921 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
4981 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
5041 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
5101 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
5161 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
5221 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
5281 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
5341 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
5401 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
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