| 质粒名称: | MAP2-GCaMP3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 43917 |
| 存储实验室: | John Gearhart |
|
载体骨架基本信息 |
|
| 载体名称: | FU-MAP2-Gateway |
| 载体出品公司: | Clontech |
| 空载体大小: | 9600 bp |
| 完整质粒大小: | 10900 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Zeocin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | GCaMP3、G-CaMP3 |
| 物种来源: | Synthetic |
| 插入大小: | 1300 bp |
| 基因突变: | E247V in GCaMP3 |
| GenBank编号 | HM143847.1 ADJ53338.1 |
| 启动子: | Human MAP2 promoter |
| 标签/融合蛋白: |
6xHis (N terminal on insert)
T7 epitope (N terminal on insert)
Xpress tag (N terminal on insert)
|
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | T7 promoter |
| 3’测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | MAP2-GCaMP3 was a gift from John Gearhart (Addgene plasmid # 43917 ; http://n2t.net/addgene:43917 ; RRID:Addgene_43917) |
| 参考文献部分: | Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD. PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub 2011 Dec 9. 10.1371/journal.pone.0028719 PubMed 22174877 |
买家导航
MAP2-GCaMP3载体质粒基本信息
MAP2-GCaMP3质粒图谱载体图谱和MAP2-GCaMP3载体序列质粒序列多克隆位点信息
MAP2-GCaMP3质粒载体简介
MAP2-GCaMP3质粒序列载体序列
LOCUS MAP2-GCaMP3 10997 bp DNA circular SYN 25-AUG-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10997)
AUTHORS Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
TITLE Efficient conversion of astrocytes to functional midbrain
dopaminergic neurons using a single polycistronic vector.
JOURNAL PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
2011 Dec 9.
PUBMED 22174877
REFERENCE 2 (bases 1 to 10997)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
date: "2011"; volume: "6(12):e28719. doi"; pages: "
10.1371/journal.pone.0028719. Epub 2011 Dec 9"
FEATURES Location/Qualifiers
source 1..10997
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 382..580
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 598..778
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 825..950
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1443..1676
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1861..1905
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2203..2320
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 3856..3875
/label=T7
/note="T7 promoter, forward primer"
promoter 3856..3874
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 3906..3930
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 3931..5283
/codon_start=1
/product="improved fluorescent protein-based calcium sensor
(Chen et al., 2013)"
/label=GCaMP6f
/translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
YGVQCFSRYPDHMKQHDFFKSAMPVGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
CDS 3931..5283
/codon_start=1
/product="improved fluorescent protein-based calcium sensor
(Chen et al., 2013)"
/label=GCaMP6s
/translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
YGVQCFSRYPDHMKQHDFFKSAMPVGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
regulatory 4027..4036
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind 4317..4338
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(4444..4465)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4705..4724)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
protein_bind complement(5284..5308)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 5334..5375
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
misc_feature 5452..6040
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5505..5525)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5923..5934)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6043..6059)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6044..6060)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6565..6745
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6771..6788)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6777..7001
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7047..7475
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7134..7153)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7344..7365
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7484..7504)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7489..7818
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7669..7804
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7731..7750
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7866..7913
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7932..8306
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8436..8557
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8473..8492)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8527..8546
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8606..8622)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8606..8622)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8619..8641)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8630..8646
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8654..8684)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8699..8720
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8837..8854)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9008..9596)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9088..9107)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9767..10627)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10390..10409
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10628..10732)
/gene="bla"
/label=AmpR promoter
primer_bind complement(10807..10826)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
2341 cagagatcca gtttggttaa ttaacagctg gcctttttgg ttctcatcga attttgctcc
2401 attcactggt tcaggttgct aacaatgctg taaaaattaa cttatttatt gacatttttc
2461 tccttccctt ttccccagga aataaatgca ggaaattatt ccacacttaa cctccctaca
2521 caagggcact gtaaaatagg gatattcaac tgctaatttc agttgctaac atggagggga
2581 tacagattat gccaaagatt gaattctctg cctgggccct cagggactga aaagccccag
2641 gaccaagctg gttgccacct ccttttgctt ttgtatgtaa atagattgcc cccagaaatc
2701 acatttctaa ttctctgttt tttttttctt tttctcttgt aaggaaaaca tggccaagtg
2761 cataattata actgttaaaa gggtattgaa ttttctgtag gaaaatatac ataaatatat
2821 gcatttcttt aatgaagata tttattttag aatataaata agcaatatgc gatagttaaa
2881 aatattgtaa ttatctgcca atgacaaaac atcaacaaat ctctgatgtc cttaatgcag
2941 ataataaaaa ttcccataaa ctagtattta gtcatgtcca gtttgtgtag cttaacttgt
3001 ttgtaatcct atatgcctga aaactgtttc tctatttaag cggtgtgtgt gtgtgcgtgc
3061 gtgcgtgcgt gtgtgtgtgt gtgtgtctgg cattaacaca aaggagacca gatgatgaca
3121 ttcataggct gatagactcg actcctgggg acacacttgg atggaggcaa ttaaaatgcc
3181 tgtcaggcat ttggtaccag aagcccctat ggcaaaccgc taaatcgtaa gtgagggctg
3241 catcctattg tcagtgaatg atggcggagc tgagtggctt gtcatcattc tcgaaaattc
3301 tgagactagt gagctctctc cctctctccc actcgcctta ttttccagtt cgccactgcg
3361 gggacttcta atttgcagct tccctttccc cggcacacac agacacgagc tggtggcttg
3421 cagactgcgt ctgtctggag ctagagagcc agagagccag cctgtgggga taatgctccc
3481 ggagaaggat tctgcagcag ttctcaaagg ctagacttga gtggtattgc tgcatatgcg
3541 ctggtaatgg tttggggctt ttaattttat ttcgtttcat tttcaattcg tttttcatag
3601 gatgtgcttt atgtaatctc agagtaaaga tcttttaaaa ttcattattg agcttcctaa
3661 atattcaagt actgcatttt ttttgtgcta cagacctcca ggcatagatt tattatttga
3721 gtataaagaa tgcatttgct tactggtgag ttataatttt tgatatttag ttaacaaatc
3781 catatatata tatatatata tgtttctttt gttttgtttc ggtagattct tcagcttgtc
3841 tctaaccgac tcgagtaata cgactcacta tagggagacc caagctggct aggtaagctt
3901 gatcaacaag tttgtacaaa aaagcaggct atgggttctc atcatcatca tcatcatggt
3961 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
4021 gatctcgcca ccatggtcga ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc
4081 agagctatag gtcggctgag ctcactcgag aacgtctata tcaaggccga caagcagaag
4141 aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
4201 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
4261 cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
4321 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
4381 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
4441 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
4501 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
4561 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
4621 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccgt aggctacatc
4681 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
4741 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
4801 ggcaacatcc tggggcacaa gctggagtac aacacgcgtg accaactgac tgaagagcag
4861 atcgcagaat ttaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca
4921 accaaggagc tggggacggt gatgcggtct ctggggcaga accccacaga agcagagctg
4981 caggacatga tcaatgaagt ggatgccgac ggtgacggca caatcgactt ccctgagttc
5041 ctgacaatga tggcaagaaa aatgaaagac acagacagtg aagaagaaat tagagaagcg
5101 ttccgtgtgt ttgataagga tggcaatggc tacatcagtg cagcagagct tcgccacgtg
5161 atgacaaacc ttggagagaa gttaacagat gaagaggttg atgaaatgat cagggaagca
5221 gacatcgatg gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag
5281 taaacccagc tttcttgtac aaagtggttg atctagaggg cccgcggttc gaaggtaagc
5341 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat cgccatcacc
5401 attgagttta aacccgctga tcagcctcaa ttcgatatca agcttatcga taatcaacct
5461 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
5521 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
5581 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
5641 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
5701 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
5761 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
5821 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
5881 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
5941 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
6001 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
6061 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
6121 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
6181 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
6241 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
6301 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
6361 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
6421 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
6481 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
6541 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
6601 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
6661 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
6721 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
6781 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
6841 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
6901 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
6961 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
7021 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
7081 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
7141 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
7201 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
7261 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
7321 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
7381 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
7441 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
7501 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
7561 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
7621 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
7681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
7741 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
7801 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
7861 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
7921 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
7981 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
8041 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
8101 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
8161 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
8221 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
8281 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
8341 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
8401 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
8461 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
8521 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
8581 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
8641 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
8701 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
8761 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
8821 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
8881 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
8941 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
9001 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
9061 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
9121 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
9181 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
9241 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
9301 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
9361 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
9421 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
9481 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
9541 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
9601 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
9661 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
9721 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
9781 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
9841 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
9901 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
9961 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
10021 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
10081 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
10141 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
10201 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
10261 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
10321 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
10381 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
10441 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
10501 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
10561 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
10621 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
10681 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
10741 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
10801 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
10861 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
10921 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
10981 ggccagatat acgcgtt
//
