| 质粒名称: | pYSEAP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37326 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | custom |
| 载体抗性: | 杀稻瘟菌素 75µg/ml |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 杀稻瘟菌素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | EF1a |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pYSEAP was a gift from John Schiller (Addgene plasmid # 37326 ; http://n2t.net/addgene:37326 ; RRID:Addgene_37326) |
| 参考文献部分: | Reactivity of human sera in a sensitive, high-throughput pseudovirus-based papillomavirus neutralization assay for HPV16 and HPV18. Pastrana DV, Buck CB, Pang YY, Thompson CD, Castle PE, FitzGerald PC, Kruger Kjaer S, Lowy DR, Schiller JT. Virology. 2004 Apr 10;321(2):205-16. 10.1016/j.virol.2003.12.027 PubMed 15051381 |
买家导航
pYSEAP载体质粒基本信息
pYSEAP质粒图谱载体图谱和pYSEAP载体序列质粒序列多克隆位点信息
pYSEAP质粒载体简介
pYSEAP质粒序列载体序列
LOCUS pYSEAP 5297 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pYSEAP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5297)
AUTHORS Pastrana DV, Buck CB, Pang YY, Thompson CD, Castle PE, FitzGerald
PC, Kruger Kjaer S, Lowy DR, Schiller JT
TITLE Reactivity of human sera in a sensitive, high-throughput
pseudovirus-based papillomavirus neutralization assay for HPV16 and
HPV18.
JOURNAL Virology. 2004 Apr 10;321(2):205-16.
PUBMED 15051381
REFERENCE 2 (bases 1 to 5297)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5297
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 462..1640
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 693..1631
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1588..1608
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 1727..3229
/codon_start=1
/product="secreted alkaline phosphatase from human
placenta"
/label=SEAP
/note="The natural enzyme has a C-terminal transmembrane
sequence that is replaced by a GPI anchor."
/translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
polyA_signal 3287..3408
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3298..3317)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3352..3371
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(3656..3673)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3827..4415)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3907..3926)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4445..4840)
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
promoter complement(4859..4906)
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter complement(4954..5284)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4968..5103
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(5022..5041)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 5269..5289
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
1 cgcgttgaca ttgattattg actagttagt tattactagc cgcgtgcaaa gcaccggcgg
61 cggtagatgc ggggtaagta ctgaatttta attcgaccta tcccggtaaa gcgaaagcga
121 cacgcttttt tttcacacat agcgggaccg aacacgttat aagtatcgaa ctcctaaaga
181 agcagtgtag ttttctgcag atgcaaaaaa gatctcatga aggaggaact tgtgcagttt
241 acttaatctg ctttaacaca gctaaaagca gagaaacagt ccggaatctg atggcaaaca
301 cgctaaatgt aagagaagag tgtttgatgc tgcagccagc taaaattcga ggactcagcg
361 cagctctatt ctggtttagc ttgcaaagat ggataaagtt ttaaacagag aggaatcttt
421 gcagctaatg gaccttgtag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
481 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
541 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
601 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
661 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
721 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
781 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
841 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
901 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
961 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
1021 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
1081 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
1141 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
1201 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
1261 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
1321 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
1381 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
1441 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
1501 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
1561 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
1621 ttccatttca ggtgtcgtga ggaattctct agagatccct cgacctcgag atccattgtg
1681 ctggatctgc gatctaagta agcttcgaat cgcgaattcg cccaccatgc tgctgctgct
1741 gctgctgctg ggcctgaggc tacagctctc cctgggcatc atcccagttg aggaggagaa
1801 cccggacttc tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc
1861 tgcacagaca gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac
1921 ggtgacagct gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc
1981 cctggccatg gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca
2041 tgtgccagac agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca
2101 gaccattggc ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga
2161 ggtcatctcc gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac
2221 cacacgagtg cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg
2281 gtactcggac gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac
2341 gcagctcatc tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt
2401 tcgcatggga accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct
2461 ggacgggaag aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg
2521 gaaccgcact gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct
2581 ctttgagcct ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct
2641 gatggagatg acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct
2701 cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact
2761 gactgagacg atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga
2821 ggacacgctg agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc
2881 cctgcgaggg agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta
2941 cacggtcctc ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga
3001 tgttaccgag agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga
3061 cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct
3121 ggttcacggc gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct
3181 ggagccctac accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc
3241 gggttactct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
3301 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
3361 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
3421 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
3481 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
3541 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
3601 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
3661 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
3721 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
3781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
3841 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
3901 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
3961 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4021 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4081 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4141 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
4201 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
4261 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
4321 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
4381 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
4441 ggtcgccctc ccacacataa ccagagggca gcaattcacg aatcccaact gccgtcggct
4501 gtccatcact gtccttcact atggctttga tcccaggatg cagatcgaga agcacctgtc
4561 ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt tccgatcgcg acgatacaag
4621 tcaggttgcc agctgccgca gcagcagcag tgcccagcac cacgagttct gcacaaggtc
4681 ccccagtaaa atgatataca ttgacaccag tgaagatgcg gccgtcgcta gagagagctg
4741 cgctggcgac gctgtagtct tcagagatgg ggatgctgtt gattgtagcc gttgctcttt
4801 caatgagggt ggattcttct tgagacaaag gcttggccat ggtttagttc ctcaccttgt
4861 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgatcaga
4921 tccgaaaatg gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa
4981 gcctcctcac tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat
5041 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
5101 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
5161 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
5221 catgctttgc atacttctgc ctgcctgggg agcctgggga ctttccacac cctaactgac
5281 acacattcca cagaatt
//
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