| 载体名称: | pcrEG |
|---|---|
| 出品公司: | Molecularcloud |
| 载体又名: | pcrEG载体，pcrEG质粒，pcrEG载体质粒 |
| 英文名称: | pcrEG, pcrEG Vector, pcrEG Plasmid |
| 质粒类型: | 细菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 3.2 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 壮观霉素 |
| 筛选标记: | sfGFP |
| 克隆菌株: | DH5α,TOP10 等 |
| 备注: | 该质粒用于在大肠杆菌中表达crRNA阵列。质粒中的sfGFP可通过同源替换进行快速筛选。 |
| 产品目录号: | MC_0101277 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pcrEG载体质粒基本信息
pcrEG质粒图谱载体图谱和pcrEG载体序列质粒序列多克隆位点信息
pcrEG质粒载体简介
pcrEG质粒序列载体序列
Snapgene格式序列: pcrEG.DNA .gb格式序列: pcrEG.gb
LOCUS pcrEG 3181 bp DNA circular SYN 18-SEP-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3181)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 18, 2025 from SnapGene 8.1.0
https://www.biofeng.com
COMMENT Sequence Label: pcrEG
FEATURES Location/Qualifiers
source 1..3181
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 124..915
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 1090..1678
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 1926..1960
/label=J23119(SpeI) promoter
/note="bacterial promoter (Registry of Standard Biological
Parts BBa_J23119) modified to end with an SpeI site"
CDS 2339..3052
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
ORIGIN
1 tcgagttcat gtgcagctcc atcagcaaaa ggggatgata agtttatcac caccgactat
61 ttgcaacagt gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat
121 gtcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
181 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
241 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
301 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
361 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
421 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
481 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
541 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
601 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
661 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
721 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
781 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
841 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
901 gtagtcggca aataagatgc cgctcgccag tcgattggct gagctcatga agttcctatt
961 ccgaagttcc gcgaacgcgt aaaggatcta ggtgaagatc ctttttgata atctcatgac
1021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
1081 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
1141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
1201 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
1261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
1321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
1381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
1441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
1501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
1561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
1621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
1681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
1741 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
1801 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
1861 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatgctg
1921 gatccttgac agctagctca gtcctaggta taatactagt agaggtagag acgcgaggtc
1981 taagaacttt aaataatttc tactgttgta gatagagacc gtgaagttaa taaggtctca
2041 aatttctact gttgtagatc gtctctgaac tgattcaagc aagcttaaac ccagctcaat
2101 gagctgggtt ttttgtttgt tttttcaaac ttagttagct tggccttatt aacgttgata
2161 taatttaaat tttatttgac aaaaatgggc tcgtgttgta caataaatgt agtgatagcg
2221 gtaccctcga agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac
2281 agcctctaca aataattttg tttaatagag ggtaagtaaa agaaaggagg tttattttat
2341 gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
2401 tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa
2461 actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt
2521 cactactctg acctatggtg ttcaatgctt ttcccgttat ccggatcaca tgaaacggca
2581 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa
2641 agatgacggg acctacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa
2701 tcgtatcgag ttaaagggta ttgattttaa agaagatgga aacattcttg gacacaaact
2761 cgagtacaac tttaactcac acaatgtata catcacggca gacaaacaaa agaatggaat
2821 caaagctaac ttcaaaattc gccacaacgt tgaagatggt tccgttcaac tagcagacca
2881 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
2941 gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct
3001 tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca aataacgaac
3061 ggcagatcag aattttgtaa taaaaaaaga gcctgctcat tacactgcgg gctctttttc
3121 atggtcagaa gacgggtaac caagataaca aagcttagat ctattaccct gttatcccta
3181 c
//
