| 质粒名称: |
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHF3-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001834 |
| 产品用途: | 3' AAVLINK plasmid for PHF3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PHF3 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHF3-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHF3-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHF3-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHF3-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHF3-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHF3-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHF3-FLAG 7029 bp DNA circular SYN 24-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7029)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 9, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7029
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3742
/codon_start=1
/gene="PHF3"
/product="PHD finger protein 3 isoform 1"
/label=PHF3
/note="isoform 1 is encoded by transcript variant 4"
/db_xref="GI:1634229891"
/db_xref="CCDS:CCDS4966.1"
/db_xref="GeneID:23469"
/db_xref="HGNC:HGNC:8921"
/db_xref="MIM:607789"
/protein_id="NP_001357277.1"
/translation="VKDMYLIPLGATDKIPHPLVPFDGPGLELHRPNLLLGLIIRQKLK
RQHSACASTSHIAETPESAPPIALPPDKKSKIEVSTEEAPEEENDFFNSFTTVLHKQRN
KPQQNLQEDLPTAVEPLMEVTKQEPPKPLRFLPGVLIGWENQPTTLELANKPLPVDDIL
QSLLGTTGQVYDQAQSVMEQNTVKEIPFLNEQTNSKIEKTDNVEVTDGENKEIKVKVDN
ISESTDKSAEIETSVVGSSSISAGSLTSLSLRGKPPDVSTEAFLTNLSIQSKQEETVES
KEKTLKRQLQEDQENNLQDNQTSNSSPCRSNVGKGNIDGNVSCSENLVANTARSPQFIN
LKRDPRQAAGRSQPVTTSESKDGDSCRNGEKHMLPGLSHNKEHLTEQINVEEKLCSAEK
NSCVQQSDNLKVAQNSPSVENIQTSQAEQAKPLQEDILMQNIETVHPFRRGSAVATSHF
EVGNTCPSEFPSKSITFTSRSTSPRTSTNFSPMRPQQPNLQHLKSSPPGFPFPGPPNFP
PQSMFGFPPHLPPPLLPPPGFGFAQNPMVPWPPVVHLPGQPQRMMGPLSQASRYIGPQN
FYQVKDIRRPERRHSDPWGRQDQQQLDRPFNRGKGDRQRFYSDSHHLKRERHEKEWEQE
SERHRRRDRSQDKDRDRKSREEGHKDKERARLSHGDRGTDGKASRDSRNVDKKPDKPKS
EDYEKDKEREKSKHREGEKDRDRYHKDRDHTDRTKSKR"
misc_feature 2844..2849
/label=SDS
misc_feature 3083..3088
/label=SDS
CDS 3749..3814
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3818..4249
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3962..3967
/label=SDS
repeat_region 4292..4432
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4292..4421
/label=AAV2 ITR
repeat_region 4293..4433
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4507..4962
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5244..5348
/gene="bla"
/label=AmpR promoter
CDS 5349..6209
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6380..6968
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtta aagatatgta
1561 ccttattcct ttgggtgcca cagataaaat tccacaccct cttgtgcctt ttgatggacc
1621 tgggcttgaa ctgcatagac ctaatctatt gttgggctta attattcgtc agaaactgaa
1681 gcgacagcac agtgcctgtg ctagtactag tcatatagct gagactcctg aaagtgcacc
1741 accaatagca ttgccacctg ataaaaaaag taaaatagaa gtttctacag aagaagcacc
1801 agaggaagaa aatgactttt ttaattcttt tacaactgta ttacacaagc agagaaataa
1861 acctcagcag aatcttcagg aagaccttcc aacagcagtt gaacctttaa tggaagtcac
1921 caaacaggag ccaccaaaac ctttaagatt tcttcctggc gtgttgattg gctgggagaa
1981 tcaacctact actctggaat tagcaaataa acctcttcct gtggatgata tacttcaaag
2041 ccttttgggc accactggtc aagtatatga ccaggcccag tcagtgatgg aacaaaacac
2101 tgttaaagaa attccatttt taaatgagca gaccaactca aaaatagaga aaacagataa
2161 tgtggaagta actgatggtg aaaacaagga gataaaagtt aaagtagata atatttcaga
2221 atctacagat aagtcagcag aaatagaaac atcagtagta gggtcctctt ccatttctgc
2281 agggtctttg acgagtctta gtctcagagg taagccacca gatgtttcta cagaagcatt
2341 tttaacaaat ttatcaattc agtcaaaaca agaggaaact gtggagagta aagagaaaac
2401 attaaaaaga cagcttcagg aagatcaaga gaataatttg caagataacc agacttcaaa
2461 tagttctcca tgcagatcta atgtaggaaa aggaaacata gatggtaatg tgagctgtag
2521 tgaaaacctt gttgctaata cagcgaggtc tccacagttt atcaacctga aaagggatcc
2581 taggcaagca gcaggacgaa gtcagcctgt aactacttca gaaagcaaag atggagatag
2641 ttgccggaat ggagaaaaac acatgctgcc tggcctgtca cacaacaagg agcacttaac
2701 agaacaaatc aatgtagagg aaaagttgtg ttctgcagag aaaaactcgt gtgttcagca
2761 gagtgacaat ttaaaagttg cacaaaactc accatcagta gaaaacatac agacttctca
2821 agcagaacaa gcaaaaccct tacaggagga tattttaatg caaaatattg aaactgtgca
2881 cccatttcga agaggatcag cagtagcgac atctcatttt gaagttggaa acacatgtcc
2941 atcagaattt ccttctaaaa gcatcacctt tacttccaga agcaccagcc ccagaacaag
3001 tacaaacttt tcacccatga ggccacagca gcccaacctt cagcatctca agtctagccc
3061 acctggattt ccatttccag ggcctcctaa ttttccccca caaagcatgt ttggatttcc
3121 accacatttg ccacctccat tacttccccc tccaggcttt ggctttgctc aaaatcccat
3181 ggttccctgg ccacctgttg ttcatctccc aggtcagcca cagcgtatga tgggtcctct
3241 ctcacaagca tcaaggtata taggcccgca gaatttttac caggttaaag acattcggag
3301 gccagaaagg cgccatagtg acccttgggg taggcaagac caacagcaac tggataggcc
3361 atttaatagg ggtaaagggg accgccagag attttatagt gattcacacc atttgaaaag
3421 agagcgacat gaaaaggaat gggagcaaga atctgaaagg catagacgca gagacagaag
3481 ccaagacaag gacagagaca gaaaaagcag ggaggaaggg cacaaagata aagagagggc
3541 acggttatca catggtgatc gaggaacaga tggaaaagca agcagagata gtaggaatgt
3601 agacaagaag ccagataaac ctaaaagtga agactatgag aaggacaaag aacgagagaa
3661 aagtaaacac agagaaggag aaaaggacag ggataggtac cacaaagata gggaccacac
3721 tgacagaact aaaagcaaaa ggtgtacaga ctacaaagac catgacggtg attataaaga
3781 tcatgacatc gactacaagg atgacgatga caagtaactc gagataatca acctctggat
3841 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
3901 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
3961 tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt
4021 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt
4081 gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga
4141 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
4201 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gatctggaga
4261 ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
4321 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
4381 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
4441 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
4501 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
4561 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
4621 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
4681 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
4741 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
4801 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
4861 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
4921 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
4981 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
5041 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
5101 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
5161 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
5221 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
5281 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
5341 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
5401 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
5461 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
5521 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
5581 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
5641 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
5701 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
5761 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
5821 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
5881 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
5941 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
6001 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
6061 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
6121 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
6181 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
6241 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
6301 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
6361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
6421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
6481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
6541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
6601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
6661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
6721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
6781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
6841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
6901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
6961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
7021 ctcacatgt
//
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