| 质粒名称: | pT7-NSP1SA11 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 89168 |
| 存储实验室: | Takeshi Kobayashi |
|
载体骨架基本信息 |
|
| 载体名称: | p3E5 |
| 载体出品公司: | -- |
| 空载体大小: | 3074 bp |
| 完整质粒大小: | 4684 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rotavirus SA11 NSP1 |
| 物种来源: | Rotavirus SA11 |
| 插入大小: | 1610 bp |
| 基因突变: | -- |
| GenBank编号 | LC178570 |
| 启动子: | T7 promotor |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7 forward CTGTGGATAACCGTATTACCG |
| 3’测序引物 | T7 terminal GCTAGTTATTGCTCAGCGG |
|
文献引用方法 |
|
| 材料和方法部分: | pT7-NSP1SA11 was a gift from Takeshi Kobayashi (Addgene plasmid # 89168 ; http://n2t.net/addgene:89168 ; RRID:Addgene_89168) |
| 参考文献部分: | Entirely plasmid-based reverse genetics system for rotaviruses. Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, Matsuura Y, Taniguchi K, Kobayashi T. Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 10.1073/pnas.1618424114. 10.1073/pnas.1618424114 PubMed 28137864 |
pT7-NSP1SA11
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pT7-NSP1SA11载体 pT7NSP1SA11质粒基本信息
pT7-NSP1SA11质粒图谱 pT7NSP1SA11载体图谱和pT7-NSP1SA11载体序列 pT7NSP1SA11质粒序列多克隆位点信息
pT7-NSP1SA11质粒 pT7NSP1SA11载体简介
pT7-NSP1SA11质粒序列 pT7NSP1SA11载体序列
LOCUS pT7-NSP1SA11 4684 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express Rotavirus SA11 NSP1 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pT7-NSP1SA11
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4684)
AUTHORS Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M,
Matsuura Y, Taniguchi K, Kobayashi T
TITLE Entirely plasmid-based reverse genetics system for rotaviruses.
JOURNAL Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi:
10.1073/pnas.1618424114.
PUBMED 28137864
REFERENCE 2 (bases 1 to 4684)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4684
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 18..36
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(74..96)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 196..215
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 425..444
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 734..752
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 734..751
/label=SP6
/note="SP6 promoter, forward primer"
terminator 820..867
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind 863..881
/label=T7 Term
/note="T7 terminator, reverse primer"
promoter complement(2636..2654)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2739..2756)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2910..3498)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2990..3009)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3669..4529)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4292..4311
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4530..4634)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
61 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
121 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
181 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat tcgacgctct
241 cccttatgcg actcctgcat taagaagcag cccagtagta ggttgaggcc gttgagcacc
301 gccgccgcaa ggaatggtgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
361 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
421 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
481 ccacgatgcg tccggcgtag aggatctggc tagcgatgac cctgctgatt ggttcgctga
541 ccatttccgg gtgcgggacg gcgttaccag aaactcagaa ggttcgtcca accaaaccga
601 ctctgacggc agtttacgag agagatgata gggtctgctt cagtaagcca gatgctacac
661 aattaggctt gtacatattg tcgttagaac gcggctacaa ttaatacata accttatgta
721 tcatacacat acgatttagg tgacactata gaatacaagc ttgcatgcct gcaggtcgac
781 tctagaggat ctcgatccgg atatagttcc tcctttcagc aaaaaacccc tcaagacccg
841 tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag ccaactcagc
901 ttcctttcgg gctttgttag cagccggatc cttttttttg agctctccct tagccatccg
961 agtggacgac gtcctccttc ggatgcccag gtcggaccgc gaggaggtgg agatgccatg
1021 ccgacccggt tcacagtatt ttgccagcta ggcgctactc tagtctggac ggtcttgtga
1081 tggctaaata ctgtataatt agatagtgac ataatttcac tcattgtcat cttctgagtt
1141 ggagatcagt agctcaaatt cttcagttaa agttccagaa ttcttcattt caatcagttc
1201 ctccgttgat tttattgaca ttggcaattt atccaattca tcaacattat accatttact
1261 gctaattgat ttaactatac tactgaattc atttaaatta agtaccattg gtatgctatc
1321 tacattcaaa gtgcgcttaa tttcattata caaactaata ttcaatccaa gaatcaacat
1381 gatgacattc gtaccttcaa ctgacactgg cgacatgtga ttaaacactt cttcaattgt
1441 ttgatccatg tgattagtaa acaaactcca aattaatctt ttaattaaag gatatatgtt
1501 ttcaacagaa ctacaatgtc caacgttaac attactttta taacaatcct taataaattc
1561 catcaataaa ttatacatat cacgaatccg ccaatcaatt ttactaagaa aatattttat
1621 attacaccat ttacagtact ggatatcgga agcattgcct gggtgattag atgccacgta
1681 gttaggctga caatgatgtc ccaactcaaa tattgtcatt ttcaaccttc caaaattatt
1741 atcatgagca tagaaccgag tatacttact cgtcatcatt gcagcaatta atttattctt
1801 atcatttttt aaatcagaat aaagctccaa ttcatctctc caattaaatt cagttacatt
1861 tctgtatatt gatcttcctg gcgtttttgt ataagtatta aaaatcacag tttctttagt
1921 aaaatctgaa aagtaaattt gctttagttt tgatgatggc agtcttgata attggaagta
1981 tttctttgcg taatggtttc tcaactctat tggtagattg gacattcttg taaagttaat
2041 atgatcaagc aataatttat caaattcatt tataaagtta acaattctat atggagcata
2101 tatatcactg actgatttct catataaccc aaaaacataa taaagattat tttcatcaaa
2161 cttaaatgca gcagcattta atgtaattgg gagcagcaaa tgattatacc attcaatttt
2221 atattgattc ctacattctc tttgctttat cattctttca aattttctaa ttactttatc
2281 attaattgga aatattatat tgtacaagtc tatgatacac tgtaatttgt ctttggtaat
2341 tggatgtttc acagttctaa gcttaagcaa atgaggttca ttgtcaagga agcatcttct
2401 gttctgaaca caccatttgc aaacatgcgg caatgagcaa ccataacaat gggttaaatc
2461 agctatttga caacattcta aacaccaccc cttaattttc gcatcaggaa ctggcatcca
2521 aatagaattt gcaccaatgt tgcataatct ccgatttaaa gcagttaatc tacgataatg
2581 aaagcatgca tctttaaaag tagccatggc taacacaaga cttttcaaaa aaaagcctat
2641 agtgagtcgt attaacccgg gatcgatatc cccctgcatt aatgaatcgg ccaacgcgcg
2701 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
2761 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
2821 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
2881 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
2941 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
3001 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
3061 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
3121 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
3181 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
3241 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
3301 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
3361 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
3421 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
3481 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
3541 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
3601 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
3661 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
3721 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
3781 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
3841 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
3901 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
3961 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
4021 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
4081 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
4141 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
4201 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
4261 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
4321 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
4381 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
4441 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
4501 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
4561 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
4621 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
4681 atca
//
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