pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc

LOCUS       pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc               11320 bp ds-DNA     circular SYN 14-DEC-2022
DEFINITION  tetO-Sox2AV-KM (Dox-inducible reprogramming lentiviral vector 
            expressing mouse Oct4 + Sox2A61V + Klf4 + cMyc).
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru
            V, Scholer HR, Velychko S
  TITLE     Enhancing Sox/Oct cooperativity induces higher-grade developmental 
            reset
  JOURNAL   bioRxiv 2022.09.23.509242
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2251..2521
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3756..3812
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4791..4844
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6315..6374
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    7762..8350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7815..7835)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8233..8244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8498..8678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8725..8743)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8811..8915
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8916..9776
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9134..9153)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9947..10535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10436..10455
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10689..10706
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10798..10817
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    10946..10967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10982..11012
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11020..11036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11025..11047
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11044..11060
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11044..11060
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11110..11129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg
     2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
     2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa
     2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg
     2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg
     2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct
     3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc
     3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc
     3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg
     3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca
     3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca
     3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa
     3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc
     3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg
     3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt
     3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc
     3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac
     3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg
     3721 gctctcccat gcattcaaac gctagcggca gcggcgccac gaacttctct ctgttaaagc
     3781 aagcaggaga tgttgaagaa aaccccgggc ctgcttgcat gtataacatg atggagacgg
     3841 agctgaagcc gccgggcccg cagcaagctt cggggggcgg cggcggagga ggcaacgcca
     3901 cggcggcggc gaccggcggc aaccagaaga acagcccgga ccgcgtcaag aggcccatga
     3961 acgccttcat ggtatggtcc cgggggcagc ggcgtaagat ggcccaggag aaccccaaga
     4021 tgcacaactc ggagatcagc aagcgcctgg gcgcggagtg gaaacttttg tccgagaccg
     4081 agaagcggcc gttcatcgac gaggccaagc ggctgcgcgt gctgcacatg aaggagcacc
     4141 cggattataa ataccggccg cggcggaaaa ccaagacgct catgaagaag gataagtaca
     4201 cgcttcccgg aggcttgctg gcccccggcg ggaacagcat ggcgagcggg gttggggtgg
     4261 gcgccggcct gggtgcgggc gtgaaccagc gcatggacag ctacgcgcac atgaacggct
     4321 ggagcaacgg cagctacagc atgatgcagg agcagctggg ctacccgcag cacccgggcc
     4381 tcaacgctca cggcgcggca cagatgcaac cgatgcaccg ctacgacgtc agcgccctgc
     4441 agtacaactc catgaccagc tcgcagacct acatgaacgg ctcgcccacc tacagcatgt
     4501 cctactcgca gcagggcacc cccggtatgg cgctgggctc catgggctct gtggtcaagt
     4561 ccgaggccag ctccagcccc cccgtggtta cctcttcctc ccactccagg gcgccctgcc
     4621 aggccgggga cctccgggac atgatcagca tgtacctccc cggcgccgag gtgccggagc
     4681 ccgctgcgcc cagtagactg cacatggccc agcactacca gagcggcccg gtgcccggca
     4741 cggccattaa cggcacactg cccctgtcgc acatggcatg cggctccggc gagggcaggg
     4801 gaagtcttct aacatgcggg gacgtggagg aaaatcccgg cccactcgag atgaggcagc
     4861 cacctggcga gtctgacatg gctgtcagcg acgctctgct cccgtccttc tccacgttcg
     4921 cgtccggccc ggcgggaagg gagaagacac tgcgtccagc aggtgccccg actaaccgtt
     4981 ggcgtgagga actctctcac atgaagcgac ttcccccact tcccggccgc ccctacgacc
     5041 tggcggcgac ggtggccaca gacctggaga gtggcggagc tggtgcagct tgcagcagta
     5101 acaacccggc cctcctagcc cggagggaga ccgaggagtt caacgacctc ctggacctag
     5161 actttatcct ttccaactcg ctaacccacc aggaatcggt ggccgccacc gtgaccacct
     5221 cggcgtcagc ttcatcctcg tcttccccag cgagcagcgg ccctgccagc gcgccctcca
     5281 cctgcagctt cagctatccg atccgggccg ggggtgaccc gggcgtggct gccagcaaca
     5341 caggtggagg gctcctctac agccgagaat ctgcgccacc tcccacggcc cccttcaacc
     5401 tggcggacat caatgacgtg agcccctcgg gcggcttcgt ggctgagctc ctgcggccgg
     5461 agttggaccc agtatacatt ccgccacagc agcctcagcc gccaggtggc gggctgatgg
     5521 gcaagtttgt gctgaaggcg tctctgacca cccctggcag cgagtacagc agcccttcgg
     5581 tcatcagtgt tagcaaagga agcccagacg gcagccaccc cgtggtagtg gcgccctaca
     5641 gcggtggccc gccgcgcatg tgccccaaga ttaagcaaga ggcggtcccg tcctgcacgg
     5701 tcagccggtc cctagaggcc catttgagcg ctggacccca gctcagcaac ggccaccggc
     5761 ccaacacaca cgacttcccc ctggggcggc agctccccac caggactacc cctacactga
     5821 gtcccgagga actgctgaac agcagggact gtcaccctgg cctgcctctt cccccaggat
     5881 tccatcccca tccggggccc aactaccctc ctttcctgcc agaccagatg cagtcacaag
     5941 tcccctctct ccattatcaa gagctcatgc caccgggttc ctgcctgcca gaggagccca
     6001 agccaaagag gggaagaagg tcgtggcccc ggaaaagaac agccacccac acttgtgact
     6061 atgcaggctg tggcaaaacc tataccaaga gttctcatct caaggcacac ctgcgaactc
     6121 acacaggcga gaaaccttac cactgtgact gggacggctg tgggtggaaa ttcgcccgct
     6181 ccgatgaact gaccaggcac taccgcaaac acacagggca ccggcccttt cagtgccaga
     6241 agtgcgacag ggccttttcc aggtcggacc accttgcctt acacatgaag aggcactttc
     6301 tcgagggctc gggccagtgt actaattatg ctctcttgaa attggctgga gatgttgaga
     6361 gcaacccagg tcccggcgcg ccactggatt tcctttgggc gttggaaacc ccgcagacag
     6421 ccacgacgat gcccctcaac gtgaacttca ccaacaggaa ctatgacctc gactacgact
     6481 ccgtacagcc ctatttcatc tgcgacgagg aagagaattt ctatcaccag caacagcaga
     6541 gcgagctgca gccgcccgcg cccagtgagg atatctggaa gaaattcgag ctgcttccca
     6601 ccccgcccct gtccccgagc cgccgctccg ggctctgctc tccatcctat gttgcggtcg
     6661 ctacgtcctt ctccccaagg gaagacgatg acggcggcgg tggcaacttc tccaccgccg
     6721 atcagctgga gatgatgacc gagttacttg gaggagacat ggtgaaccag agcttcatct
     6781 gcgatcctga cgacgagacc ttcatcaaga acatcatcat ccaggactgt atgtggagcg
     6841 gtttctcagc cgctgccaag ctggtctcgg agaagctggc ctcctaccag gctgcgcgca
     6901 aagacagcac cagcctgagc cccgcccgcg ggcacagcgt ctgctccacc tccagcctgt
     6961 acctgcagga cctcaccgcc gccgcgtccg agtgcattga cccctcagtg gtctttccct
     7021 acccgctcaa cgacagcagc tcgcccaaat cctgtacctc gtccgattcc acggccttct
     7081 ctccttcctc ggactcgctg ctgtcctccg agtcctcccc acgggccagc cctgagcccc
     7141 tagtgctgca tgaggagaca ccgcccacca ccagcagcga ctctgaagaa gagcaagaag
     7201 atgaggaaga aattgatgtg gtgtctgtgg agaagaggca aacccctgcc aagaggtcgg
     7261 agtcgggctc atctccatcc cgaggccaca gcaaacctcc gcacagccca ctggtcctca
     7321 agaggtgcca cgtctccact caccagcaca actacgccgc acccccctcc acaaggaagg
     7381 actatccagc tgccaagagg gccaagttgg acagtggcag ggtcctgaag cagatcagca
     7441 acaaccgcaa gtgctccagc cccaggtcct cagacacgga ggaaaacgac aagaggcgga
     7501 cacacaacgt cttggaacgt cagaggagga acgagctgaa gcgcagcttt tttgccctgc
     7561 gtgaccagat ccctgaattg gaaaacaacg aaaaggcccc caaggtagtg atcctcaaaa
     7621 aagccaccgc ctacatcctg tccattcaag cagacgagca caagctcacc tctgaaaagg
     7681 acttattgag gaaacgacga gaacagttga aacacaaact cgaacagctt cgaaactctg
     7741 gtgcataaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     7801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8161 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8341 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8401 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatca
     8461 cctgcaggac aggcgcgccc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8521 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8581 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8641 cctcagaccc ttttagtcag tgtggaaaat ctctagcacc cgggcgatta aggaaagggc
     8701 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     8761 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8821 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8881 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8941 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9001 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9061 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9121 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     9181 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9241 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9301 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9361 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9421 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9481 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9541 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9601 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9661 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9721 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9781 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9841 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9901 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9961 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10021 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10081 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10141 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10201 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10261 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10321 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10381 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10441 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10501 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10561 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10621 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10681 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10741 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
    10801 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    10861 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
    10921 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10981 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    11041 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
    11101 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    11161 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    11221 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11281 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//