| 质粒名称: | pQL280 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002537 |
| 产品用途: | Synthetic PhE2F-driven mCherry expression vector (PhE2F-mCherry-pA) |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | Synthetic |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | mCherry |
| 物种来源: | Discosoma |
| 密码子优化: | 未优化 |
pQL280
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pQL280载体质粒基本信息
pQL280质粒图谱载体图谱和pQL280载体序列质粒序列多克隆位点信息
pQL280质粒载体简介
pQL280质粒序列载体序列
LOCUS pQL280 5726 bp DNA circular SYN 08-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 5726)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5726
/mol_type="other DNA"
/organism="recombinant plasmid"
primer_bind 214..254
/label=QL776 F
primer_bind 214..233
/label=QL750 F
misc_feature 234..558
/label=E2F-1
regulatory 512..521
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(539..578)
/label=QL777 R
primer_bind complement(540..567)
/label=QL751 R
primer_bind 559..585
/label=QL137-F
CDS 565..567
/codon_start=1
/product="E1A"
/label=E1A
/note="other spliced forms exist with larger introns"
/protein_id="DAA00639.1"
/translation="M"
CDS 565
/codon_start=1
/product="start codon"
/label=ATG
/translation=""
CDS 568..1272
/codon_start=1
/label=mCherry
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind 632..651
/label=mcherry-F
primer_bind complement(732..751)
primer_bind complement(1252..1297)
/label=QL138-R
primer_bind complement(1253..1284)
/label=QL10 mCherry-R
primer_bind complement(1255..1276)
/label=QL144-R
terminator 1323..1550
/label=bGH PA
primer_bind complement(1560..1582)
/label=QL215 R
misc_feature 2218..2295
/label=SV40 ORI
CDS 2437..3228
/label=Kan/neoR
primer_bind complement(3568..3588)
/label=M13-rev
misc_binding complement(3594..3616)
/label=LacO
promoter complement(3621..3650)
/label=lac
CDS complement(4733..5392)
/label=AmpR
promoter complement(5632..5660)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtggtacca
241 tccggacaaa gcctgcgcgc gccccgcccc gccattggcc gtaccgcccc gcgccgccgc
301 cccatcccgc ccctcgccgc cgggtccggc gcgttaaagc caataggaac cgccgccgtt
361 gttcccgtca cggccggggc agccaattgt ggcggcgctc ggcggctcgt ggctctttcg
421 cggcaaaaag gatttggcgc gtaaaagtgg ccgggacttt gcaggcagcg gcggccgggg
481 gcggagcggg atcgagccct cgccgaggcc tgccgccatg ggcccgcgcc gccgccgccg
541 cctgtcaccc gggccgcgga attcatggtg agcaagggcg aggaggataa catggccatc
601 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
661 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
721 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
781 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
841 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
901 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
961 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
1021 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
1081 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
1141 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
1201 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
1261 gagctgtaca agtaagcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
1321 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
1381 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
1441 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
1501 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
1561 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
1621 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
1681 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
1741 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
1801 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
1861 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
1921 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
1981 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
2041 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
2101 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
2161 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
2221 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
2281 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
2341 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
2401 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
2461 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
2521 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
2581 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
2641 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
2701 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
2761 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
2821 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
2881 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
2941 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
3001 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
3061 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
3121 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
3181 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
3241 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
3301 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
3361 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
3421 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
3481 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
3541 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
3601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
3661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
3721 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
3781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
3841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
3901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
3961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
4021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
4081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
4141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
4201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
4261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
4321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
4381 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
4441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
4501 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
4561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
4621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
4681 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
4741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
4861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
4921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
4981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
5041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
5101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
5161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
5221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
5281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
5341 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
5401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
5461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
5521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
5581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
5641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
5701 ttccccgaaa agtgccacct gacgtc
//
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