pCMV-Luciferase-12xMS2

LOCUS       pCMV-Luciferase-12xMS2           6402 bp DNA     circular SYN 31-AUG-2026
DEFINITION  Localization: C1 Cloning Vector, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    3059-pCMV-Luciferase-12xMS2V5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6402)
  TITLE     Michael Davidson Empty Backbones
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        142..445
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        446..649
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     600..620
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(673..708)
                     /label=引物 6
     primer_bind     679..717
                     /label=引物 1
     regulatory      688..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..2346
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2323..2361)
                     /label=Luc-MS2-R
     primer_bind     2332..2370
                     /label=MS2-F
     misc_feature    2347..3081
                     /label=HSPA5-intron 2-from 1386
     misc_feature    2372..2383
                     /label=BstXI
     misc_feature    2384..2690
                     /label=6xMS2_V5-1
     misc_feature    2384..2404
                     /label=Feature 50
     misc_feature    2399..2421
                     /label=MS2V5-1
     misc_feature    2426..2447
                     /label=1367, TS1
     misc_feature    2452..2474
                     /label=MS2V5-2
     misc_feature    2479..2500
                     /label=1367
     misc_feature    2505..2527
                     /label=MS2V5-3
     misc_feature    2532..2553
                     /label=1367
     misc_feature    2558..2580
                     /label=MS2V5-4
     misc_feature    2585..2606
                     /label=1367
     misc_feature    2611..2633
                     /label=MS2V5-5
     misc_feature    2638..2659
                     /label=1367
     misc_feature    2664..2686
                     /label=MS2V5-6
     misc_feature    2680..2690
                     /label=Feature 51
     misc_feature    2691..2712
                     /label=1367
     misc_feature    2691..2712
                     /label=6xMS2_V5-1
     misc_feature    2717..2739
                     /label=MS2V5-7
     misc_feature    2744..2765
                     /label=1367
     misc_feature    2770..2792
                     /label=MS2V5-8
     misc_feature    2797..2818
                     /label=1367
     misc_feature    2823..2845
                     /label=MS2V5-9
     misc_feature    2850..2871
                     /label=1367
     misc_feature    2876..2898
                     /label=MS2V5-10
     misc_feature    2903..2924
                     /label=1367
     misc_feature    2929..2951
                     /label=MS2V5-11
     misc_feature    2956..2977
                     /label=1367
     misc_feature    2978..2994
                     /label=Feature 53
     misc_feature    2982..3004
                     /label=MS2V5-12
     misc_feature    3009..3030
                     /label=1367
     misc_feature    3035..3046
                     /label=BstXI
     primer_bind     complement(3058..3096)
                     /label=MS2-R
     primer_bind     3067..3105
                     /label=MS2-Vec-F
     polyA_signal    3271..3392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3308..3327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3362..3381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3399..3854)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3536..3557)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3748..3767
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3881..3985
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3987..4344
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(4010..4030)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4195..4330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4257..4276
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4280..4291)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4379..5173
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4433..4452)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5043..5062
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5361..5380)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5405..5452
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5781..6369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6270..6289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
       61 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
      121 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      181 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      241 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      301 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      361 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      421 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      481 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      541 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      601 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
      661 ccgtcagatc cgctagcgct accggtcgcc accatggaag atgccaaaaa cattaagaaa
      721 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct
      781 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
      841 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga
      901 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt
      961 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat
     1021 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc
     1081 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt
     1141 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca
     1201 tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac
     1261 aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct
     1321 ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat
     1381 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg
     1441 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa
     1501 gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca
     1561 accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta
     1621 cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag
     1681 aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt
     1741 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt
     1801 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa
     1861 ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac
     1921 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac
     1981 gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg
     2041 gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc
     2101 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac
     2161 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg
     2221 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa
     2281 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg caagatcgcc
     2341 gtgtaagtaa gtatgaaatt cagggatacg gccaccttgt tggccggtaa cctacaaacg
     2401 ggtggaggat caccccaccc gacacgcacc gatgctctcc gaggaggcac aagggtggag
     2461 gaacacccca ccctccaggc accgatgctc tccgaggagg caaacagaag caccatcagg
     2521 gcttctgcta cgcaccgatg ctctccgagg aggaacaagg aatcaccatc agggattccc
     2581 tgtggcaccg atgctctccg aggaggacga cgggaggacg atcacgcctc ccgaatagca
     2641 ccgatgctct ccgaggaggt cagtgcgtgg agcatcagcc cacgcagcca gcaccgatgc
     2701 tctccgagga ggctttcgcg aagagcatca gccttcgcgc catgcaccga tgctctccga
     2761 ggaggcctac aggaacagca tcagcgttcc tgcccagcac cgatgctctc cgaggaggtg
     2821 tccccatgca gcatcagcgc atgggccccg caccgatgct ctccgaggag gcacatccga
     2881 gcaccaacag ggctcggagt gtgcaccgat gctctccgag gaggaccctc catggaccat
     2941 caggccatgg actctgcacc gatgctctcc gaggaggctt ctcgaagcag catcagcgct
     3001 tcgaaacagc accgatgctc tccgaggagg ttctccacct tgttggcata tttgccaaat
     3061 agtggaaatg tgaagtactg atccggactc agatctcgag ctcaagcttc gaattctgca
     3121 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat
     3181 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     3241 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     3301 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3361 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     3421 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3481 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3541 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3601 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3661 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3721 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3781 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3841 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3901 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3961 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     4021 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4081 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4141 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4201 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4261 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4321 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     4381 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4441 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4501 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4561 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4621 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4681 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4741 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4801 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4861 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4921 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4981 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5041 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5101 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5161 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5221 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5281 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5341 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     5401 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     5461 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     5521 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     5581 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     5641 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5701 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5761 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5821 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5881 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5941 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6001 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6061 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6121 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6181 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6241 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6301 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6361 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tg
//