pAAVLINK-EF1a-HA-FLNC-A

LOCUS       pAAVLINK-EF1a-HA-FLNC-A               6788 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        146..688
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             737..3928
                     /codon_start=1
                     /label=FLNC
                     /translation="MMNNSGYSDAGLGLGDETDEMPSTEKDLAEDAPWKKIQQNTFTRW
                     CNEHLKCVGKRLTDLQRDLSDGLRLIALLEVLSQKRMYRKFHPRPNFRQMKLENVSVAL
                     EFLEREHIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWEDEDDEDARKQTPKQ
                     RLLGWIQNKVPQLPITNFNRDWQDGKALGALVDNCAPGLCPDWEAWDPNQPVENAREAM
                     QQADDWLGVPQVIAPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPVRSKQLNPKKAIAY
                     GPGIEPQGNTVLQPAHFTVQTVDAGVGEVLVYIEDPEGHTEEAKVVPNNDKDRTYAVSY
                     VPKVAGLHKVTVLFAGQNIERSPFEVNVGMALGDANKVSARGPGLEPVGNVANKPTYFD
                     IYTAGAGTGDVAVVIVDPQGRRDTVEVALEDKGDSTFRCTYRPAMEGPHTVHVAFAGAP
                     ITRSPFPVHVSEACNPNACRASGRGLQPKGVRVKEVADFKVFTKGAGSGELKVTVKGPK
                     GTEEPVKVREAGDGVFECEYYPVVPGKYVVTITWGGYAIPRSPFEVQVSPEAGVQKVRA
                     WGPGLETGQVGKSADFVVEAIGTEVGTLGFSIEGPSQAKIECDDKGDGSCDVRYWPTEP
                     GEYAVHVICDDEDIRDSPFIAHILPAPPDCFPDKVKAFGPGLEPTGCIVDKPAEFTIDA
                     RAAGKGDLKLYAQDADGCPIDIKVIPNGDGTFRCSYVPTKPIKHTIIISWGGVNVPKSP
                     FRVNVGEGSHPERVKVYGPGVEKTGLKANEPTYFTVDCSEAGQGDVSIGIKCAPGVVGP
                     AEADIDFDIIKNDNDTFTVKYTPPGAGRYTIMVLFANQEIPASPFHIKVDPSHDASKVK
                     AEGPGLNRTGVEVGKPTHFTVLTKGAGKAKLDVQFAGTAKGEVVRDFEIIDNHDYSYTV
                     KYTAVQQGNMAVTVTYGGDPVPKSPFVVNVAPPLDLSKIKVQGLNSKVAVGQEQAFSVN
                     TRGAGGQGQLDVRMTSPSRRPIPCKLEPGGGAEAQAVRYMPPEEGPYKVDITYDGHPVP
                     GSPFAVEGVLPPDPSK"
     misc_feature    1701..1706
                     /label=SDS
     misc_feature    3831..3836
                     /label=SDS
     intron          3929..3969
                     /label=Splicing Donor
     misc_feature    3973..4006
                     /label=loxP-JT1571
     repeat_region   4051..4191
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4051..4180
                     /label=AAV2 ITR
     rep_origin      4266..4721
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5003..5107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5108..5968
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6139..6727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctatga tgaacaacag cggctactca gacgccggcc tcggcctggg
      781 cgatgagaca gacgagatgc cgtccacgga gaaggacctg gcggaggacg cgccgtggaa
      841 gaagatccag cagaacacat tcacgcgctg gtgcaatgag cacctcaagt gcgtgggcaa
      901 gcgcctgacc gacctgcagc gcgacctcag cgacgggctc cggctcatcg cgctgctcga
      961 ggtgctcagc cagaagcgca tgtaccgcaa gttccatccg cgccccaact tccgccaaat
     1021 gaagctggag aacgtgtccg tggccctcga gttcctcgag cgcgagcaca tcaagctcgt
     1081 gtccatagac agcaaggcca tcgtggatgg gaacctgaag ctgatcctgg gcctgatctg
     1141 gacgctgatc ctgcactact ccatctccat gcccatgtgg gaggatgaag atgatgagga
     1201 tgcccgcaaa cagacgccca agcagcggct gcttggctgg atccagaaca aggtgcccca
     1261 gctgcccatc accaacttca accgtgactg gcaggacggc aaagctctgg gcgccctggt
     1321 ggacaactgc gcccccggtc tctgccccga ctgggaggcc tgggacccca accagcccgt
     1381 ggagaacgcc cgggaggcca tgcagcaggc cgacgactgg cttggggtgc cccaggtcat
     1441 tgcccctgag gagattgtgg accccaacgt ggatgagcat tctgttatga cctacctgtc
     1501 ccagttcccc aaggccaagc tcaaacctgg tgcccctgtt cgatccaagc agctgaaccc
     1561 caagaaagcc atcgcctatg ggcctggcat cgagccacag ggcaacaccg tgctgcagcc
     1621 tgcccacttc accgtgcaga cggtggacgc gggcgtgggc gaggtgctgg tctacatcga
     1681 ggaccctgaa ggccacaccg aggaggctaa ggtggttccc aacaatgaca aggatcgcac
     1741 ctatgctgtc tcctatgtgc ccaaggtcgc tgggttacac aaggtgaccg tgctctttgc
     1801 tggccagaac attgaacgca gtccctttga ggtgaacgtg ggcatggccc tgggagatgc
     1861 caacaaggtg tcagcccgtg gccctggcct ggaacctgtg ggcaatgtgg ccaacaaacc
     1921 cacctacttt gacatctaca ctgcgggggc cggcactggc gatgttgctg tggtgatcgt
     1981 ggacccacag ggccggcggg acacagtgga ggtggccctg gaggacaagg gtgacagcac
     2041 gttccgctgc acatacagac ctgccatgga ggggccacat accgtgcatg tggcctttgc
     2101 gggtgccccc atcacccgca gtcccttccc tgtccatgtg tcggaagcct gtaaccccaa
     2161 cgcctgccgc gcctctgggc gaggcctgca gcccaagggt gttcgcgtga aagaggtggc
     2221 tgacttcaag gtgtttacca agggtgccgg cagcggggag ctcaaggtca cggtcaaggg
     2281 gccaaagggc acagaggagc cagtgaaggt gcgggaggct ggggatggtg tgttcgagtg
     2341 cgagtactac ccggtggtgc ctgggaagta tgtggtgacc atcacgtggg gcggctacgc
     2401 catccctcgc agcccctttg aggtacaggt gagcccagag gcaggagtgc aaaaggtccg
     2461 ggcctggggt cctggtttgg agactggcca ggtgggcaag tcagccgatt ttgtggtgga
     2521 agccattggc accgaggtgg ggacactggg cttctccatc gaggggccct cacaagccaa
     2581 gatcgaatgt gacgacaagg gggatggctc ctgcgatgtg cggtactggc ccacggagcc
     2641 tggggagtac gctgtgcacg tcatctgtga cgatgaggac atccgagact cacccttcat
     2701 tgcccacatc ctgcccgccc cacctgactg cttcccagat aaggtgaagg cctttgggcc
     2761 tggcctggag cctaccggct gcatcgtgga caagcccgct gagttcacca ttgatgctcg
     2821 tgcagctggc aagggagacc tgaagctcta tgcccaggac gccgacggct gtcccatcga
     2881 catcaaggtg atccccaacg gcgacggcac cttccgctgc tcctacgtgc ccaccaagcc
     2941 cattaagcac accatcatca tctcctgggg aggcgtaaac gtgcccaaga gccccttccg
     3001 ggtgaacgtg ggcgagggca gccaccccga gcgggtaaag gtgtacggcc ccggagtgga
     3061 gaagacaggc ctcaaggcca atgagcccac ctacttcacg gtggactgca gcgaggcggg
     3121 gcaaggcgac gtgagcatcg gcatcaagtg cgccccaggc gtggtgggcc ctgcagaggc
     3181 tgacattgac ttcgacatca tcaagaatga caacgacacc ttcaccgtca agtacacgcc
     3241 accaggggcg ggccgctaca ccatcatggt gctgtttgcc aaccaggaga tccccgccag
     3301 ccccttccac atcaaggtgg acccatccca cgatgccagc aaagtcaagg ccgagggccc
     3361 tgggctgaat cgcacaggtg tggaagtcgg gaagcccacc cacttcacgg tgctgaccaa
     3421 gggagccggc aaggccaagc tggatgtgca gtttgcaggg acagccaagg gcgaggttgt
     3481 gcgggacttt gagatcatag acaaccatga ctactcctac actgtcaagt acaccgctgt
     3541 ccagcagggc aacatggcag tgacagtgac ttatggcggg gaccctgtcc ccaagagccc
     3601 ctttgtggtg aatgtggcac ccccgctgga cctcagcaaa atcaaagttc agggccttaa
     3661 tagcaaggtg gctgtgggac aggaacaagc attctctgtg aacacacgag gggctggcgg
     3721 tcagggccaa ctggatgtgc ggatgacttc gccctctcgc cggcccatcc cctgcaagct
     3781 ggagccaggc ggtggagcgg aagcccaggc tgtgcgctac atgcccccgg aggaggggcc
     3841 ctacaaggtg gatatcacct acgatggtca cccggtgcct ggcagcccgt ttgctgtgga
     3901 gggtgtcctg ccccctgatc cctccaaggt aagtatcaag gttacaagac aggtttaagg
     3961 agaccaataa gctattattc gtatagcata cattatacga agttattaag ttatggagac
     4021 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4081 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4141 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4201 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4261 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4321 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     4381 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4441 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4501 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4561 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     4621 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     4681 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     4741 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4801 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4861 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     4921 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4981 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5221 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5281 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5341 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5401 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5461 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5521 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5581 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5641 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5701 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5761 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5821 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5881 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5941 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6241 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6781 tcacatgt
//