pCambia2300-RFP

LOCUS       pCAMBIA2300-RFP        10138 bp    DNA     circular SYN 29-DEC-2018
DEFINITION  Binary vector pCAMBIA-2300-RFP, complete sequence.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12618270|
COMMENT     VNTDBDATE|-12618270|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCAMBIA2300-RFP|
COMMENT     VNTAUTHORNAME|Ming He|
COMMENT     VNTAUTHOREML|826388375@qq.com|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    complement(980..1980)
                     /vntifkey="21"
                     /label=pVS1\STA
                     /note="STA region from pVS1 plasmid"
     rep_origin      complement(2574..3574)
                     /vntifkey="33"
                     /label=pVS1-REP
                     /note="pVS1-REP; replication origin from pVS1"
     misc_feature    complement(3984..4244)
                     /vntifkey="21"
                     /label=pBR322\Bom\site
                     /note="bom site from pBR322"
     rep_origin      complement(4384..4664)
                     /vntifkey="33"
                     /label=pBR322\origin
                     /note="pBR322 origin of replication"
     CDS             complement(4955..5749)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAF65399.1"
                     /db_xref="GI:7638147"
                     /vntifkey="4"
                     /label=Kana\Resistance
                     /note="aadA (kanamycin resistance) gene amplified from pIG121Hm"
     misc_feature    6174..6199
                     /vntifkey="21"
                     /label=left\border\repeat\from\C58\T-DNA
                     /note="left border repeat from C58 T-DNA"
     misc_feature    6266..6469
                     /vntifkey="21"
                     /label=CaMV\3'UTR\(polyA\signal)
                     /note="CaMV 3'UTR (polyA signal)"
     CDS             complement(6507..7304)
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /protein_id="AAF65400.1"
                     /db_xref="GI:7638148"
                     /vntifkey="4"
                     /label=NptII
                     /note="nptII  gene"
     promoter        complement(7335..8115)
                     /vntifkey="30"
                     /label=CaMV\35S\promoter,\duplicated
                     /note="CaMV 35S promoter, duplicated"
     promoter        8263..8317
                     /vntifkey="30"
                     /label=lacZ\promoter
                     /note="PlacZ; lacZ promoter"
     misc_feature    10052..10077
                     /vntifkey="21"
                     /label=Right\border\T-DNA\repeat
                     /note="right border T-DNA repeat"
     promoter        8365..8895
                     /vntifkey="29"
                     /label=CAMV\35S\Promoter
     misc_feature    8923..9600
                     /vntifkey="21"
                     /label=RFP
BASE COUNT     2408 a      2811 c      2730 g      2189 t 
ORIGIN
        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct 
       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca 
      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 
      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 
      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 
      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 
      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 
      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 
      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 
      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 
      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg 
      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 
      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 
      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 
      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 
      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 
      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 
     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg 
     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 
     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 
     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 
     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 
     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 
     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 
     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 
     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 
     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 
     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 
     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 
     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 
     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 
     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 
     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 
     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 
     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 
     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 
     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 
     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 
     2281 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg 
     2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg 
     2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag 
     2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc 
     2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc 
     2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc 
     2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac 
     2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca 
     2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg 
     2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg 
     2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg 
     2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg 
     3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc 
     3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc 
     3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac 
     3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt 
     3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag 
     3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc 
     3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg 
     3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga 
     3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa 
     3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg 
     3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc 
     3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct 
     3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc 
     3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt 
     3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 
     3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 
     3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 
     4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 
     4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 
     4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     4921 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat 
     4981 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat 
     5041 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact 
     5101 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa 
     5161 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg 
     5221 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt 
     5281 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt 
     5341 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact 
     5401 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa 
     5461 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca 
     5521 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca 
     5581 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt 
     5641 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt 
     5701 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc 
     5761 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc 
     5821 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt 
     5881 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc 
     5941 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg 
     6001 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa 
     6061 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta 
     6121 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg 
     6181 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt 
     6241 ttaatgtact gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt 
     6301 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa 
     6361 gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct 
     6421 gggaactact cacacattat tatggagaaa ctcgagcttg tcgatcgact ctagctagag 
     6481 gatcgatccg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg 
     6541 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg 
     6601 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc 
     6661 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc 
     6721 ggcaagcagg catcgccatg tgtcacgacg agatcctcgc cgtcgggcat gcgcgccttg 
     6781 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga 
     6841 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg 
     6901 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg 
     6961 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc 
     7021 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg 
     7081 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct ggagttcatt cagggcaccg 
     7141 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg 
     7201 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa 
     7261 gcggccggag aacctgcgtg caatccatct tgttcaatcc ccatggtcga tcgacagatc 
     7321 tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct 
     7381 ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc 
     7441 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg 
     7501 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc 
     7561 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc 
     7621 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag 
     7681 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac 
     7741 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca 
     7801 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg 
     7861 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt 
     7921 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg 
     7981 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt 
     8041 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt 
     8101 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg 
     8161 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 
     8221 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 
     8281 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 
     8341 agctatgacc atgattacga attcccatgg agtcaaagat tcaaatagag gacctaacag 
     8401 aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc aatgacaaga 
     8461 agaaaatctt cgtcaacatg gtggagcacg acacgcttgt ctactccaaa aatatcaaag 
     8521 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa 
     8581 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg 
     8641 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct 
     8701 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag 
     8761 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg 
     8821 atgacgcaca atcccactat cctttgcaag acccttcctc tatataagga agttcatttc 
     8881 atttggagag gacagggtac ccggggatcc tctagagtcg acatggcctc ctccgaggac 
     8941 gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag 
     9001 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 
     9061 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttccag 
     9121 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 
     9181 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 
     9241 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 
     9301 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 
     9361 accgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagat gaggctgaag 
     9421 ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc 
     9481 gtgcagctgc ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag 
     9541 gactacacca tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgcctaa 
     9601 ctgcaggcat gccctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc 
     9661 tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg 
     9721 ccggtttcgg ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat 
     9781 attctccgtt caatttactg attgaagctt ggcactggcc gtcgttttac aacgtcgtga 
     9841 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 
     9901 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 
     9961 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa 
    10021 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta 
    10081 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtg 
//