pH7GWIWG2(I)

LOCUS       pH7GWIWG2(I)               13291 bp DNA     circular SYN 13-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13291)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..13291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    263..387
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        412..442
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             794..1099
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1140..1264)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1639..2298
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    2701..2825
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2866..3171)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(3523..3553)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3578..3702)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3811..4155)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    4848..4872
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6172..6801
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7235..8302
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8368..8562
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8906..9046
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9232..9820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10064..10855)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    11383..11407
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(11500..11752)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(13008..13187)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 ctagagggcc cgacgtcgca tgcctgcagg tcactggatt ttggttttag gaattagaaa
       61 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
      121 acacatgagc gaaaccctat aagaacccta attcccttat ctgggaacta ctcacacatt
      181 attctggaga aaaatagaga gagatagatt tgtagagaga gactggtgat ttttgcggac
      241 tctagcatgg ccgcgggata tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      301 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      361 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      421 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccggctta ctaaaagcca
      481 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
      541 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga
      601 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
      661 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
      721 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
      781 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      841 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
      901 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
      961 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     1021 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     1081 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     1141 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     1201 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     1261 tggtgatatc actagtgcgg ccgcctgcag gtcgaccata tggtcgacct gcaggcggcc
     1321 gcactagtga tgctgttatg ttcagtgtca agctgacctg caaacacgtt aaatgctaag
     1381 aagttagaat atatgagaca cgttaactgg tatatgaata agctgtaaat aaccgagtat
     1441 aaactcatta actaatatca cctctagagt ataatataat caaattcgac aatttgactt
     1501 tcaagagtag gctaatgtaa aatctttata tatttctaca atgttcaaag aaacagttgc
     1561 atctaaaccc ctatggccat caaattcaat gaacgctaag ctgatccggc gagattttca
     1621 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     1681 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     1741 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     1801 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     1861 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     1921 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     1981 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     2041 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     2101 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     2161 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     2221 gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     2281 tggcagggcg gggcgtaaac gcgtggatca gcttaatatg actctcaata aagtctcata
     2341 ccaacaagtg ccaccttatt caaccatcaa gaaaaaagcc aaaatttatg ctactctaag
     2401 gaaaacttca ctaaagaaga cgatttagag tgttttacca agaatttctg tcatcttact
     2461 aaacaactaa agatcggtgt gatacaaaac ctaatctcat taaagtttat gctaaaataa
     2521 gcataatttt acccactaag cgtgaccaga taaacataac tcagcacacc agagcatata
     2581 tattggtggc tcaaatcata gaaacttaca gtgaagacac agaaagccgt aagaagaggc
     2641 aagagtatga aaccttacct catcatttcc atgaggttgc ttctgatccc gcgggatatc
     2701 accactttgt acaagaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     2761 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     2821 ctatggtcga cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa
     2881 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
     2941 cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
     3001 cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact
     3061 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
     3121 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagt
     3181 ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc
     3241 cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg
     3301 ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc
     3361 gctgtcaact gtcactgtaa tacgctgctt catagcacac ctctttttga catacttcgg
     3421 gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt
     3481 tatctggctt ttagtaagcc ggatcctaac tcaaaatcca cacattatac gagccggaag
     3541 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
     3601 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     3661 atcattttac gtttctcgtt cagctttttt gtacaaactt gtgatatcac tagtgcggcc
     3721 gcctgcaggt cgactagaat agtaaattgt aatgttgttt gttgtttgtt ttgttgtggt
     3781 aattgttgta aaaatacgga tcgtcctgca gtcctctcca aatgaaatga acttccttat
     3841 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     3901 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     3961 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     4021 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     4081 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     4141 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     4201 tagacgagag tgtcgtgctc caccatgttg acgaagattt tcttcttgtc attgagtcgt
     4261 aaaagactct gtatgaactg ttcgccagtc ttcacggcga gttctgttag atcctcgatc
     4321 tgaatttttg actccatggc ctttgattca gtaggaacta ctttcttaga gactccaatc
     4381 tctattactt gccttggttt atgaagcaag ccttgaatcg tccatactgg aatagtactt
     4441 ctgatcttga gaaatatatc tttctctgtg ttcttgatgc agttagtcct gaatcttttg
     4501 actgcatctt taaccttctt gggaaggtat ttgatctcct ggagattatt actcgggtag
     4561 atcgtcttga tgagacctgc cgcgtaggcc tctctaacca tctgtgggtc agcattcttt
     4621 ctgaaattga agaggctaat cttctcatta tcggtggtga acatggtatc gtcaccttct
     4681 ccgtcgaact ttcttcctag atcgtagaga tagagaaagt cgtccatggt gatctccggg
     4741 gcaaaggaga tcagcttggc tctagtcgac catatgggag agctcaagct tagcttgagc
     4801 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     4861 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt
     4921 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     4981 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     5041 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     5101 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     5161 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     5221 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     5281 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     5341 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     5401 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     5461 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     5521 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     5581 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     5641 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     5701 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     5761 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     5821 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     5881 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     5941 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     6001 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     6061 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     6121 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     6181 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     6241 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     6301 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     6361 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     6421 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     6481 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     6541 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     6601 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     6661 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     6721 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     6781 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     6841 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     6901 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     6961 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     7021 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     7081 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     7141 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     7201 actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca
     7261 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     7321 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     7381 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     7441 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     7501 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     7561 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     7621 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     7681 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     7741 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     7801 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     7861 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     7921 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     7981 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     8041 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     8101 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     8161 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     8221 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     8281 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     8341 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     8401 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     8461 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     8521 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     8581 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     8641 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     8701 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     8761 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     8821 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     8881 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     8941 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     9001 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     9061 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9661 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga
     9901 tatatctccc aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt
     9961 gacctgatag tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac
    10021 gccggcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct
    10081 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
    10141 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
    10201 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
    10261 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
    10321 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
    10381 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
    10441 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
    10501 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
    10561 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
    10621 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
    10681 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
    10741 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
    10801 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt
    10861 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
    10921 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
    10981 caaaaaaaca tgtcataaca agaagccatg aaaaccgcca ctgcgccgtt accaccgctg
    11041 cgttcggtca aggttctgga ccagttgcgt gacggcagtt acgctacttg cattacagct
    11101 tacgaaccga acgaggctta tgtccactgg gttcgtgccc gaattgatca caggcagcaa
    11161 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
    11221 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
    11281 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
    11341 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
    11401 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
    11461 aattaacgcc gaattgaatt atcagcttgc atgccggtcg atctagtaac atagatgaca
    11521 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
    11581 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
    11641 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
    11701 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tctgcttgac
    11761 tctagggaat taattcctga atcactgcga ccggccctcc cgcgacccag ccgagcgagc
    11821 ttagcgaact gtggacgaga actgtgccac caagcgtaag gccgttctct cgcatttgcc
    11881 ttgctaggct cgcgcgagtt gctggctgag gcgttctcga aatcagctct tgttcggtcg
    11941 gcatctactc tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc
    12001 gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg
    12061 cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc
    12121 atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat
    12181 atacgcccgg agccttggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt
    12241 ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg
    12301 ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt
    12361 caggacattg ttggagccga atccgcgtgc acgagtgccg gacttcgggg cagtcctcgc
    12421 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
    12481 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
    12541 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
    12601 cgcagcgata gcatccatag cctccgcgac cggctgaaga acagcgggca gttcggtttc
    12661 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
    12721 gctgaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
    12781 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
    12841 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
    12901 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
    12961 aggctttttc atagggggga ttcgagttga gagtgaatat gagactctaa ttggataccg
    13021 aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag
    13081 tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt
    13141 aaactccaga aacccgcggc tcagtggctc cttcaacgtt gcggttctgt cagttccaaa
    13201 cgtaaaacgg cttgtcccgc gtcatcggcg gggtcataac gtgactccct taattctcat
    13261 gtatgataat tcgagggtac ccggggatcc t
//