pCAMBIA1105.1

价格:1800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: CAMBIA
载体名称: pCAMBIA1105.1
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lac
载体大小: 12137 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: --
载体抗性: 氨基糖苷类抗生素
筛选标记: Hygromycin、GUS
克隆菌株: HB101等菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCAMBIA1105.1载体图谱



pCAMBIA1105.1 多克隆位点

载体简介

载体序列

LOCUS       pCAMBIA1105.1          12137 bp ds-DNA     circular SYN 25-JUL-2016
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            hygromycin- and spectinomycin-resistance and GUSPlus(TM) genes plus 
            the pUC19 MCS.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12137)
  AUTHORS   Cambia
  TITLE     Direct Submission
  JOURNAL   Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector 
            NTI(R) format
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 4740.
COMMENT     VNTDATE|658713600|
COMMENT     VNTDBDATE|776304000|
COMMENT     VNTNAME|pCAMBIA1105.1|
COMMENT     VNTAUTHORNAME|Cambia|
FEATURES             Location/Qualifiers
     CDS             join(2..16,207..2024)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
     intron          17..206
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2031..2048
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
     terminator      2080..2332
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2354..2378
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3678..4307
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
     CDS             4736..5809
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5875..6069
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6413..6553
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6739..7327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7571..8362)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase 氨基糖苷类抗生素"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
     misc_feature    8923..8947
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9025..9199
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9239..10264)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
     promoter        complement(10332..11009)
                     /label=35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        11236..11266
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11274..11290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11298..11314
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11310..11543
                     /codon_start=1
                     /gene="lacZ (truncated)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
     misc_feature    11324..11380
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(11384..11400)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11777..12122
                     /label=35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus
                     CaMV 35S promoter"
ORIGIN
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgaactag tctgtacccg atcaacaccg
      241 agacccgtgg cgtcttcgac ctcaatggcg tctggaactt caagctggac tacgggaaag
      301 gactggaaga gaagtggtac gaaagcaagc tgaccgacac tattagtatg gccgtcccaa
      361 gcagttacaa tgacattggc gtgaccaagg aaatccgcaa ccatatcgga tatgtctggt
      421 acgaacgtga gttcacggtg ccggcctatc tgaaggatca gcgtatcgtg ctccgcttcg
      481 gctctgcaac tcacaaagca attgtctatg tcaatggtga gctggtcgtg gagcacaagg
      541 gcggattcct gccattcgaa gcggaaatca acaactcgct gcgtgatggc atgaatcgcg
      601 tcaccgtcgc cgtggacaac atcctcgacg atagcaccct cccggtgggg ctgtacagcg
      661 agcgccacga agagggcctc ggaaaagtca ttcgtaacaa gccgaacttc gacttcttca
      721 actatgcagg cctgcaccgt ccggtgaaaa tctacacgac cccgtttacg tacgtcgagg
      781 acatctcggt tgtgaccgac ttcaatggcc caaccgggac tgtgacctat acggtggact
      841 ttcaaggcaa agccgagacc gtgaaagtgt cggtcgtgga tgaggaaggc aaagtggtcg
      901 caagcaccga gggcctgagc ggtaacgtgg agattccgaa tgtcatcctc tgggaaccac
      961 tgaacacgta tctctaccag atcaaagtgg aactggtgaa cgacggactg accatcgatg
     1021 tctatgaaga gccgttcggc gtgcggaccg tggaagtcaa cgacggcaag ttcctcatca
     1081 acaacaaacc gttctacttc aagggctttg gcaaacatga ggacactcct atcaacggcc
     1141 gtggctttaa cgaagcgagc aatgtgatgg atttcaatat cctcaaatgg atcggcgcca
     1201 acagcttccg gaccgcacac tatccgtact ctgaagagtt gatgcgtctt gcggatcgcg
     1261 agggtctggt cgtgatcgac gagactccgg cagttggcgt gcacctcaac ttcatggcca
     1321 ccacgggact cggcgaaggc agcgagcgcg tcagtacctg ggagaagatt cggacgtttg
     1381 agcaccatca agacgttctc cgtgaactgg tgtctcgtga caagaaccat ccaagcgtcg
     1441 tgatgtggag catcgccaac gaggcggcga ctgaggaaga gggcgcgtac gagtacttca
     1501 agccgttggt ggagctgacc aaggaactcg acccacagaa gcgtccggtc acgatcgtgc
     1561 tgtttgtgat ggctaccccg gagacggaca aagtcgccga actgattgac gtcatcgcgc
     1621 tcaatcgcta taacggatgg tacttcgatg gcggtgatct cgaagcggcc aaagtccatc
     1681 tccgccagga atttcacgcg tggaacaagc gttgcccagg aaagccgatc atgatcactg
     1741 agtacggcgc agacaccgtt gcgggctttc acgacattga tccagtgatg ttcaccgagg
     1801 aatatcaagt cgagtactac caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg
     1861 tgggtgagca agcgtggaac ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc
     1921 aaggaaacaa gaagggcgtg ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct
     1981 ttcgcgagcg ctggaccaac attccagatt tcggctacaa gaacgctagc catcaccatc
     2041 accatcacgt gtgaattggt gaccagctcg aatttccccg atcgttcaaa catttggcaa
     2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
     2341 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2401 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     2461 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     2521 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     2581 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     2641 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     2701 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     2761 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     2821 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     2881 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     2941 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     3001 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     3061 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     3121 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     3181 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     3241 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     3301 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     3361 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     3421 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     3481 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     3541 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     3601 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     3661 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     3721 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     3781 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     3841 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     3901 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     3961 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     4021 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     4081 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     4141 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     4201 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     4261 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     4321 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     4381 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     4441 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     4501 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     4561 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     4621 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     4681 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     4741 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     4801 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     4861 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     4921 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     4981 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     5041 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     5101 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     5161 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     5221 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     5281 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     5341 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     5401 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     5461 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     5521 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     5581 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     5641 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     5701 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     5761 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     5821 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     5881 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     5941 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     6001 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     6061 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     6121 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     6181 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     6241 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     6301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     6361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     6421 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     6481 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     6541 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7381 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
     7441 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
     7501 ttagtgcatc taatcgcttg agttaacgcc ggcgaagcgg cgtcggcttg aacgaatttc
     7561 tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     7621 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     7681 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     7741 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     7801 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     7861 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     7921 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     7981 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     8041 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     8101 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     8161 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     8221 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     8281 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     8341 cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg
     8401 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     8461 tggatgcccg aggcatagac tgtaccccaa aaaaacatgt cataacaaga agccatgaaa
     8521 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgac
     8581 ggcagttacg ctacttgcat tacagcttac gaaccgaacg aggcttatgt ccactgggtt
     8641 cgtgcccgaa ttgatcacag gcagcaacgc tctgtcatcc gcggtgatca caggcagcaa
     8701 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
     8761 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
     8821 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
     8881 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
     8941 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
     9001 aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa
     9061 ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat
     9121 atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc
     9181 acacattatt atggagaaac tcgagcttgt cgatcgacag atccggtcgg catctactct
     9241 atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     9301 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     9361 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     9421 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     9481 gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     9541 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     9601 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     9661 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     9721 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     9781 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     9841 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     9901 tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt
     9961 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctaaact
    10021 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
    10081 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
    10141 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
    10201 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
    10261 tcatatctca ttgccccccg ggatctgcga aagctcgaga gagatagatt tgtagagaga
    10321 gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt
    10381 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
    10441 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
    10501 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
    10561 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
    10621 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
    10681 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
    10741 cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
    10801 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
    10861 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
    10921 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
    10981 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
    11041 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc
    11101 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    11161 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    11221 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    11281 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcg agctcggtac
    11341 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc gtcgttttac
    11401 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    11461 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    11521 gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc
    11581 ttcagtttag cttcatggag tcaaagattc aaatagagga cctaacagaa ctcgccgtaa
    11641 agactggcga acagttcata cagagtctct tacgactcaa tgacaagaag aaaatcttcg
    11701 tcaacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
    11761 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
    11821 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
    11881 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
    11941 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
    12001 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
    12061 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagaa
    12121 cacgggggac tcttgac
//

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