| 出品公司: | CAMBIA |
|---|---|
| 载体名称: | pCAMBIA0105.1R |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 10033 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 氨基糖苷类抗生素 |
| 筛选标记: | GUS |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAMBIA0105.1R 10033 bp ds-DNA circular SYN 25-JUL-2016
DEFINITION Agrobacterium binary vector for plant transformation, with
spectinomycin-resistance and GUSPlus(TM) genes.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10033)
AUTHORS Cambia
TITLE Direct Submission
JOURNAL Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector
NTI(R) format
http://www.snapgene.com
COMMENT The sequence was corrected by inserting a G at position 4740.
COMMENT VNTDATE|658800000|
COMMENT VNTDBDATE|776304000|
COMMENT VNTNAME|pCAMBIA0105.1R|
COMMENT VNTAUTHORNAME|Cambia|
FEATURES Location/Qualifiers
CDS join(2..16,207..2024)
/codon_start=1
/product="beta-glucuronidase"
/label=GUSPlus(TM)
/note="codon-optimized Staphylococcus gusA gene with a
catalase intron to ensure expression in plants but not
bacteria"
intron 17..206
/label=cat1 intron
/note="castor bean catalase intron, modified"
CDS 2031..2048
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
terminator 2080..2332
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2354..2378
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 3678..4307
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label=pVS1 StaA
CDS 4736..5809
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 5875..6069
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 6413..6553
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(6739..7327)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7571..8362)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase氨基糖苷类抗生素"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
misc_feature 8923..8947
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 9051..9081
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9089..9105
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9113..9129
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 9125..9439
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
misc_feature 9142..9247
/label=MCS
/note="multiple cloning site"
promoter complement(9255..9273)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(9280..9296)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 9673..10018
/label=35S promoter
/note="CaMV 35S promoter。strong constitutive promoter from
cauliflower mosaic virus"
ORIGIN
1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
181 gtctatgatg atgatgatag ttacagaacc gacgaactag tctgtacccg atcaacaccg
241 agacccgtgg cgtcttcgac ctcaatggcg tctggaactt caagctggac tacgggaaag
301 gactggaaga gaagtggtac gaaagcaagc tgaccgacac tattagtatg gccgtcccaa
361 gcagttacaa tgacattggc gtgaccaagg aaatccgcaa ccatatcgga tatgtctggt
421 acgaacgtga gttcacggtg ccggcctatc tgaaggatca gcgtatcgtg ctccgcttcg
481 gctctgcaac tcacaaagca attgtctatg tcaatggtga gctggtcgtg gagcacaagg
541 gcggattcct gccattcgaa gcggaaatca acaactcgct gcgtgatggc atgaatcgcg
601 tcaccgtcgc cgtggacaac atcctcgacg atagcaccct cccggtgggg ctgtacagcg
661 agcgccacga agagggcctc ggaaaagtca ttcgtaacaa gccgaacttc gacttcttca
721 actatgcagg cctgcaccgt ccggtgaaaa tctacacgac cccgtttacg tacgtcgagg
781 acatctcggt tgtgaccgac ttcaatggcc caaccgggac tgtgacctat acggtggact
841 ttcaaggcaa agccgagacc gtgaaagtgt cggtcgtgga tgaggaaggc aaagtggtcg
901 caagcaccga gggcctgagc ggtaacgtgg agattccgaa tgtcatcctc tgggaaccac
961 tgaacacgta tctctaccag atcaaagtgg aactggtgaa cgacggactg accatcgatg
1021 tctatgaaga gccgttcggc gtgcggaccg tggaagtcaa cgacggcaag ttcctcatca
1081 acaacaaacc gttctacttc aagggctttg gcaaacatga ggacactcct atcaacggcc
1141 gtggctttaa cgaagcgagc aatgtgatgg atttcaatat cctcaaatgg atcggcgcca
1201 acagcttccg gaccgcacac tatccgtact ctgaagagtt gatgcgtctt gcggatcgcg
1261 agggtctggt cgtgatcgac gagactccgg cagttggcgt gcacctcaac ttcatggcca
1321 ccacgggact cggcgaaggc agcgagcgcg tcagtacctg ggagaagatt cggacgtttg
1381 agcaccatca agacgttctc cgtgaactgg tgtctcgtga caagaaccat ccaagcgtcg
1441 tgatgtggag catcgccaac gaggcggcga ctgaggaaga gggcgcgtac gagtacttca
1501 agccgttggt ggagctgacc aaggaactcg acccacagaa gcgtccggtc acgatcgtgc
1561 tgtttgtgat ggctaccccg gagacggaca aagtcgccga actgattgac gtcatcgcgc
1621 tcaatcgcta taacggatgg tacttcgatg gcggtgatct cgaagcggcc aaagtccatc
1681 tccgccagga atttcacgcg tggaacaagc gttgcccagg aaagccgatc atgatcactg
1741 agtacggcgc agacaccgtt gcgggctttc acgacattga tccagtgatg ttcaccgagg
1801 aatatcaagt cgagtactac caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg
1861 tgggtgagca agcgtggaac ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc
1921 aaggaaacaa gaagggcgtg ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct
1981 ttcgcgagcg ctggaccaac attccagatt tcggctacaa gaacgctagc catcaccatc
2041 accatcacgt gtgaattggt gaccagctcg aatttccccg atcgttcaaa catttggcaa
2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
2341 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
2401 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
2461 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
2521 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
2581 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
2641 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
2701 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
2761 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
2821 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
2881 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
2941 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
3001 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
3061 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
3121 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
3181 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
3241 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
3301 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
3361 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
3421 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
3481 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
3541 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
3601 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
3661 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
3721 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
3781 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
3841 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
3901 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
3961 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
4021 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
4081 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
4141 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
4201 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
4261 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
4321 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
4381 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
4441 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
4501 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
4561 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
4621 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
4681 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
4741 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
4801 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
4861 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
4921 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
4981 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
5041 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
5101 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
5161 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
5221 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
5281 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
5341 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
5401 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
5461 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
5521 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
5581 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
5641 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
5701 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
5761 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
5821 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
5881 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
5941 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
6001 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
6061 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
6121 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
6181 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
6241 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
6301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
6361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
6421 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
6481 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
6541 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
6601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
6661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
6721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
6781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
6841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
6901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
6961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
7021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
7081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
7141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
7201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
7261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
7321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
7381 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
7441 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
7501 ttagtgcatc taatcgcttg agttaacgcc ggcgaagcgg cgtcggcttg aacgaatttc
7561 tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
7621 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
7681 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
7741 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
7801 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
7861 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
7921 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
7981 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
8041 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
8101 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
8161 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
8221 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
8281 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
8341 cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg
8401 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
8461 tggatgcccg aggcatagac tgtaccccaa aaaaacatgt cataacaaga agccatgaaa
8521 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgac
8581 ggcagttacg ctacttgcat tacagcttac gaaccgaacg aggcttatgt ccactgggtt
8641 cgtgcccgaa ttgatcacag gcagcaacgc tctgtcatcc gcggtgatca caggcagcaa
8701 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
8761 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
8821 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
8881 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
8941 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
9001 aattaacgcc gaattaatgt gagttagctc actcattagg caccccaggc tttacacttt
9061 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
9121 agctatgacc atgattacgc caagcttggt accgagctcg gatccactag taacggccgc
9181 cagtgtgctg gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag
9241 agggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
9301 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
9361 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
9421 cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca
9481 gtttagcttc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
9541 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
9601 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
9661 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
9721 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
9781 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
9841 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
9901 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
9961 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg
10021 ggggactctt gac
//
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