| 出品公司: | Agilent |
|---|---|
| 载体名称: | pShuttle-IRES-hrGFP-1 |
| 质粒类型: | 腺病毒表达载体，哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8.9 kb |
| 5' 测序引物及序列: | pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC |
| 3' 测序引物及序列: | pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG |
| 载体标签: | -- |
| 载体抗性: | 卡那 |
| 备注: | 载体内包含有IRES序列和hrGFP序列。 |
| 产品目录号: | 240081 |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 腺病毒 |
买家导航
pShuttle-IRES-hrGFP-1载体质粒基本信息
pShuttle-IRES-hrGFP-1质粒图谱载体图谱和pShuttle-IRES-hrGFP-1载体序列质粒序列多克隆位点信息
pShuttle-IRES-hrGFP-1质粒载体简介
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).
