pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL5A1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL5A1-FLAG            6936 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3649
                     /codon_start=1
                     /label=COL5A1
                     /translation="GPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPT
                     GPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGE
                     TGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGG
                     PPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQD
                     GPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGP
                     PGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSG
                     PKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLP
                     GPPGPKGAKGSSGPTGPKGEAGHPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDD
                     GNGENYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEY
                     WVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLL
                     SYVDAEGNPVGVVQMTFLRLLSASAHQNVTYHCYQSVAWQDAATGSYDKALRFLGSNDE
                     EMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEV
                     GPACFMG"
     misc_feature    1667..1672
                     /label=SDS
     misc_feature    2387..2392
                     /label=SDS
     misc_feature    2720..2725
                     /label=SDS
     CDS             3656..3721
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3725..4156
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3869..3874
                     /label=SDS
     repeat_region   4199..4339
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4199..4328
                     /label=AAV2 ITR
     rep_origin      4414..4869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5151..5255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5256..6116
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6287..6875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggc ctgtggggct
     1561 cccgggtcca gctggccctg tgggtccccc tggagaagac ggagataagg gagagatcgg
     1621 ggagccgggg cagaaaggaa gcaaggggga caaaggagaa cagggtcctc ctgggcctac
     1681 aggtcctcaa ggccccatcg gacagccagg cccctctgga gctgacggcg agccggggcc
     1741 tcggggccag cagggccttt tcgggcagaa aggtgatgaa ggtcccagag gctttcctgg
     1801 accccctggg ccagtggggc tgcagggttt gccaggacct ccaggcgaga agggtgagac
     1861 aggagacgtg ggccagatgg gccccccggg tccccctggc ccccgaggac cctccggagc
     1921 tccaggtgct gatggcccac aaggtccccc aggtggaata ggaaaccctg gtgcagtggg
     1981 agagaagggc gagcctggcg aagcaggtga gcctggcctt ccgggagaag gcggcccccc
     2041 gggacccaaa ggagaaaggg gagagaaggg cgagtcaggc ccttcaggtg ctgccggacc
     2101 ccctggaccc aaaggccctc ccggagatga tggtcccaaa ggcagccctg gcccagtggg
     2161 ttttcctgga gatcctggcc cccccggaga gcctggcccc gcgggtcaag atggtccccc
     2221 tggtgacaaa ggagatgatg gtgaacccgg gcagacggga tcccccggcc ctactggtga
     2281 accaggtcca tcggggcctc caggaaaaag gggtccccca ggccccgcag gccccgaagg
     2341 cagacaggga gagaaagggg ccaagggaga agccggcttg gaaggccctc ctgggaagac
     2401 tggccccatc ggcccccagg gggcccctgg gaagcccgga ccggatggcc ttcgagggat
     2461 ccctggccct gtgggagaac aaggtctccc aggatcccca ggcccggacg gtccccccgg
     2521 ccccatgggt cccccaggac ttcccggcct caaaggagat tctggtccca aaggtgaaaa
     2581 gggtcatcca ggcctgatcg ggctcatcgg tcctcccggt gaacagggtg agaagggcga
     2641 ccgtggtctc cctggccccc agggctcctc cggtcctaag ggagaacagg gtatcactgg
     2701 tccttctggc ccgattgggc ctcctgggcc ccctggcctg ccgggtccgc ctggtccaaa
     2761 aggtgctaag ggctcctcgg gtccaactgg cccgaagggt gaggcaggcc acccaggacc
     2821 cccaggcccc ccgggccccc cgggagaggt catccagccc ctgccaatcc aggcatccag
     2881 gacgcggcgg aacatcgacg ccagccagct gctggacgac gggaatggcg agaactacgt
     2941 ggactacgcg gacggcatgg aagagatctt cggctctctc aactctctga agctggagat
     3001 tgagcagatg aaacggcccc tgggcacgca gcagaacccc gcccgcacct gcaaggacct
     3061 gcagctctgc caccccgact tcccagatgg tgaatactgg gtcgatccta accaaggatg
     3121 ctccagggat tccttcaagg tttactgcaa cttcacagcc ggggggtcga catgcgtctt
     3181 ccctgacaag aagtccgaag gggccagaat cacttcttgg cccaaagaaa acccgggctc
     3241 ctggttcagt gaattcaagc gtgggaaact gctctcctat gtggacgccg agggcaaccc
     3301 tgtgggtgtg gtacagatga ccttcctgcg gctgctgagc gcctctgccc accagaacgt
     3361 cacctaccac tgctaccagt cagtggcctg gcaggacgca gccacgggca gctacgacaa
     3421 ggccctccgc ttcctgggct ccaacgacga ggagatgtcc tatgacaaca acccctacat
     3481 ccgcgccctg gtggacggct gtgctaccaa gaaaggctac cagaagacgg ttctggagat
     3541 cgacaccccc aaagtggagc aggtgcccat cgtggacatc atgttcaatg acttcggtga
     3601 agcgtcacag aaatttggat ttgaagtggg gccggcttgc ttcatgggct gtacagacta
     3661 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     3721 gtaactcgag ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3781 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3841 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc
     3901 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3961 actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4021 atctagcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4081 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     4141 gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag
     4201 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4261 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4321 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4381 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     4441 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     4501 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4561 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4621 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4681 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4741 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     4801 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     4861 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4921 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4981 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5041 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5101 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6421 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6901 ggttcctggc cttttgctgg ccttttgctc acatgt
//