| 质粒名称: | pRiA4-VIR |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 138193 |
| 存储实验室: | Qi-Jun Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pRiA4 |
| 完整质粒大小: | 33041 bp |
| 载体类型: | Vir gene expression |
| 载体抗性: | Spectinomycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | vir gene cluster |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | unknown (unknown if destroyed) |
| 3’克隆位点: | unknown (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pRiA4-VIR was a gift from Qi-Jun Chen (Addgene plasmid # 138193 ; http://n2t.net/addgene:138193 ; RRID:Addgene_138193) |
| 参考文献部分: | A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. 10.1104/pp.19.00767 PubMed 31558579 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRiA4-VIR 33041 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Virulence helper compatible with pCambia binary vectors..
ACCESSION .
VERSION .
KEYWORDS pRiA4-VIR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 33041)
AUTHORS Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ
TITLE A Novel Ternary Vector System United with Morphogenic Genes Enhances
CRISPR/Cas Delivery in Maize.
JOURNAL Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi:
10.1104/pp.19.00767.
PUBMED 31558579
REFERENCE 2 (bases 1 to 33041)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..33041
/organism="synthetic DNA construct"
/mol_type="other DNA"
RBS 1..9
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 12878..12889
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 22829..27464
/label=pRi replicator region
/note="replicator region from Agrobacterium plasmid pRiA
4b"
primer_bind complement(27504..27526)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 27612..27752
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 27667..27686
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(27767..27784)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(27938..28526)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(28018..28037)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(28782..29573)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
1 taaggaggtg cgcattgatg cgatgctttg agagataccg tttacatcta aatcgcctct
61 cgctctcgaa tgcgatgatg cgcgtgatat cgagctgcgc cccaagcttg tgcggtgcaa
121 ttgcatggag catttcctca tccggacccg ccgcagcgca atctgcgggt ggcggcactg
181 accccgccac aatggttaac aatatatgca cgtttatcct tggtccgttc ggccagtcac
241 tcgctgttct cggcattgtc gctatcggga tctcctggat gttcgggcgg gcttcgcttg
301 ggctggttgc cggcgtcgtc ggcggcattg ttatcatgtt tggggcgagc ttcctcggcc
361 aaacgctcac tggcggtagt tgatggctga tcgtttggaa gaatcgaccc tttacctcgc
421 agccacacgg cccgcattgt ttcttggggt gccactgaca ttggcagggt tattcatgat
481 gttcgccggc tttgtcatcg ttatcgttca gaacccgctc tacgaagtcg ttctcgtgcc
541 gttatggttt gcagcccggc tcatcgtgga gcgagactac aatgcggcga gcgtcgtcct
601 gctatttttg cggaccgcgg gaagaagcat tgatagtgca gtttgggggg gcgctactgt
661 tagcccaaat ccaattaggg ttcccccacg agggagagga atggtgtgat gctcggcgcg
721 agtggaacga ccgaaagatc cggtgagatc tatctccctt atattggcca cctcagcgac
781 catatcgtcc ttcttgaaga cggatcgatc atgaccattg cgagaattga tggcgttgca
841 ttcgagcttg aggaaactga aatgcgcaat gcgcgttgtc gtgcgttcaa cacgctgttg
901 cgcaatatcg ctgatgatca tgtgtcaata tatgctcacc tcgtacgtca tgccgacgtg
961 ccatcatcgg cgccgcgaca cttccgtagt gttttcgccg ctagcctgaa cgaagctttt
1021 gaacagcgcg tgctctccgg ccaactcctc cgcaatgaac acttccttac gttgattgtc
1081 tacccacagg cggctttagg gaaggtaaag aggaggttca ccaagctaag cggaaaaagg
1141 gaaaacgatc tcacgggcca gatcaggaac atggaagatc tttggcatgt tgtcgctggc
1201 tctcttaaag cgtatggcct gcatcgtctt ggcatccgcg agaagcaggg tgtgctcttc
1261 accgaaattg gcgaagcgct acggttgatc atgactggtc ggttcacacc ggttccggtc
1321 gtcagcggct cactcggcgc ttcgatttat accgacagag tcatttgcgg caagcgagga
1381 ctcgagatca gaacgccaaa agacagttac gttggatcca tctattcgtt tcgcgaatac
1441 cctgcaaaaa cacggccggg catgctcaac gcgctgctat ccctcgattt tccacttgtt
1501 ctcacgcaga gtttttcgtt cctgactcgc ccgcaagcgc acgcgaaact tagcctcaaa
1561 tcgagccaga tgctgagttc cggcgataaa gccgtgactc aaatcggcaa attatccgag
1621 gctgaggacg cacttgcgag caacgaattc gttatgggct cacatcattt gagcctttgc
1681 gtctatgcag acgatctcaa tagtcttggg gacaggggcg cgcgggctcg gacacgaatg
1741 gcggatgctg gagccgtggt tgtccaagaa ggtattggta tggaagcggc ctattggtcc
1801 caattgccgg ggaattttaa gtggcgcaca cgccctggcg caatcacttc acgcaatttc
1861 gcagggtttg tctctttcga aaactttcca gagggcgcca gctcaggcca ctggggcaac
1921 gcgattgccc gatttcgtac caatggcgga acgcctttcg actatatccc gcatgagcac
1981 gatgttggca tgacggcaat attcgggcct atcgggaggg gtaagacgac gctcatgatg
2041 tttgttctag ccatgctcga acagagcatg gtcgaccgtg caggtacggt cgtgttcttt
2101 gacaaggacc ggggtggcga attgctggtt cgcgccacag gaggaacata tttggcactt
2161 cacagaggca cacccagcgg gttggcgccg ttgcgtggcc tagaaaacac agcagcctca
2221 cacgattttc tgcgcgaatg gatcgtggct ctcatcgaga gtgatggtcg gggtgggatt
2281 tctccggaag agaaccgccg tctggtccgt ggtatccatc gtcagctctc gtttgatcca
2341 caaatgcgtt caatcgcggg gttacgtgaa tttttgttgc atgggcccgc cgaaggcgca
2401 ggagcgcggc tccaacgctg gtgccggggc catgcgcttg gctgggcatt tgacggcgaa
2461 gttgacgaag taaagttaga tccgtcgatt accggcttcg acatgacgca tcttctcgaa
2521 tacgaggaag tatgcgctcc cgctgcagca tatctcctgc atcggattgg agccatgatc
2581 gacggccgcc gttttgtgat gagctgcgat gagtttcgcg cctatttgtt aaaccctaaa
2641 ttttcgactg tcgtcgacaa attcctcctg accgttcgaa aaaacaacgg gatgctaata
2701 ctggcaacgc agcaaccaga gcatgttctg gaatcgccgc taggagccag cttggttgcg
2761 caatgtatga cgaagatttt ctatccatca ccaaccgcag atcgatcggc ttatgtcgat
2821 ggactgaaat gtaccgaaaa ggaatttcag gcgatccgtg aagacatgac ggtcggcagc
2881 cgtaagtttc ttcttaaacg agaaagtgga agcgtcatct gcgaatttga tctgcgggat
2941 atgcgtgaat atgtcgccgt gctttcgggg cgtgccaaca cggtgcgctt tgcaactcga
3001 ctacgcgagg cacaagaagg caactcatct ggctggctca gcgaattcat ggcccgtcac
3061 cacgaggcag aagattgata aggtaggaaa cgatgaagac gacgcaactt attgcaacag
3121 ttttgacctg cagctttcta tatattcagc ccgcgcgggc gcagtttgtt gttagcgacc
3181 cggcaacgga ggctgagacg ctcgcgactg cgctcgcgac tgcggagaat ctcactcaga
3241 ctatagcgat ggttacgatg ttgacgtcgg cctacggcgt tactggacta ctgacttcgc
3301 tcaaccagaa aaatcagtat ccttcgacga aggacctaga caatgaaatg ttttcgccgc
3361 gaatgccaat gtcgaccacg gcacgtgcga tcaccagcga tacagatcgt gcagtcgtgg
3421 gtagtgatgc tgaagcggac ctgttgcgat cgcagatcac cggttccgca aacagcgctg
3481 gcattgcggc tgacaatctg gaaacgatgg acaaacgctt gacggcgaat gctgatacgt
3541 ctgctcagct ttcccgatct cgcaatatca tgcaggcaac cgtgaccaat ggtttgcttc
3601 tcaagcagat ccatgacgca atgattcaaa atgtacaggc gacaagccta ttaacgatga
3661 ctaccgcgca ggccggcctt cacgaggcgg aagaggcggc cgctcaacgc aaggagcatc
3721 aaaagaccgc tgtcatcttt ggtgccctcc cctaaggctg ggcgatttgt tcatccgccc
3781 gcatcctcgc cgaatgcgag ctcattttat ccaacattat gcgacaaacc agtcaagttc
3841 aggtccaatc gatgaatttc acgattccgg cgccgtttac ggccattcat acgatcttcg
3901 atgtagcctt cacgacaggc ttggactcga tgcttgagac tatccaggag gcggtgagtg
3961 cgccattgat cgcctgtgtc actctttgga ttattgttca gggtatttta gtcatacgcg
4021 gcgaagtcga tacccgtagc ggtatcactc gggtgatcac ggtcaccatc gttgttgctc
4081 taattgttgg gcaggctaac taccaagact atgtggtttc catcttcgaa aagacggtcc
4141 caaactttgt tcagcagttt agtgtaaccg gcttgcctct gcagactgtt ccggcacagt
4201 tggatacaat gttcgccgtg acccaggccg tttttcagaa aatcgcatcc gaaatcggtc
4261 cgatgaacga ccaggacatc cttgctttcc aaggggcaca gtgggtcctt tacggcacgc
4321 tctggtctgc cttcggagtt tacgacgccg ttggaattct cacgaaagtg cttctcgcga
4381 tcgggcctct gatcctcgtc ggatatattt ttgatcgcac gcgggacatc gcagctaagt
4441 ggatcgggca acttatcacc tacggtctct tgcttctcct cttaaacctc gtggcaacga
4501 tcgtcatcct aaccgaagcg actgcgctca cccttatgct tggtgtaatc accttcgccg
4561 gtacgaccgc ggccaagatc attggtcttt acgaactcga tatgtttttt ctgacagggg
4621 atgcgctcat tgtcgctttg ccggcgatcg ccggcaacat tggaggcagt tactggagcg
4681 gcgcaaccca atctgccagc agcttgtacc gtcgcttcgc tcaggttgag cgaggctagg
4741 tcgcgcaaaa attcgcctca atggagaatt ctatgaagta ttgcctgctg tgcctagttg
4801 tcgctttgag cggctgccag acaaacgaca cattagcgag ctgcaaaggc ccgatcttcc
4861 cgctgaatgt ggggcgatgg cagcctactc cgtcagatct tcagctcggc aattcgggtg
4921 gacgctatga cggggcctga atatgccatg ctagtggcgc gcgaaagcct tgccgagcac
4981 tataaggaag tagaagcctt tcaaaccgcg cgagcgaaat cggcgcgacg tctctccaaa
5041 ctcattgcag ctgtcgcagc tatcgcgatt ttgggaaatg ttgctcaagc gttcgctata
5101 gccacaatgg tgccgttgag caggcttgtg cccgtatatc tatggatacg gccggacggc
5161 accgttgaca gcgaggtgtc tgtctcgcga ttgcctgcaa ctcaagagga ggccgtcgtt
5221 aacgcctcat tgtgggagta cgttcgcctg cgcgagagtt atgatgccga caccgctcag
5281 tacgcctacg acctggtatc gaacttcagt gccccaacag tgcgccagga ttaccagcaa
5341 ttcttcaact atcccaatcc cagttcgcct caagtcattc ttggcaaacg cggcagggtg
5401 gaggtcgagc acatcgcttc aaatgatgta actccaagca cgcagcaaat tcgctataaa
5461 aggaccctcg tcgttgacgg caaaatgcct gtggtgagta cgtggaccgc gacagttcgc
5521 tacgaaaagg tgaccagctt gcccggcaga ttgagactaa ccaacccggc aggtctggtt
5581 gtcacctcct atcagacatc ggaagatacc gtttcaaacg taggccacag cgaaccatga
5641 tcagaaaagc acttttcatt ttagcatgtt tatttgccgc tgcgactggt gcggaggctg
5701 aagacactcc aatggcgggc aagctagatc cgcgcatgcg ttatttggct tacaatcccg
5761 atcaagtggt gcgcctctcg acggcggttg gagctacttt ggtcgtaaca ttcgccacga
5821 acgaaacggt gacagcggtt gccgtttcaa atagcaaaga tctagcagcc ctaccgcggg
5881 gaaattatct atttttcaag gcaagccagg tcctcacgcc tcagccagta atcgtgctaa
5941 ccgcaagcga ctccgggatg cgccgttatg ttttcagtat aagttccaag actctgtccc
6001 acctcgataa agagcagccc gatctctatt acagcgtcca attcgcctac cccgccgacg
6061 atgcggcggc tcggcgaagg gaggcacaac agaaggctgt tgtggacaga ctacacgcgg
6121 aagcacaata tcaacggaaa gctgagaatt tattggatca gcctgtcaca gcccttggtg
6181 cggcggacag taattggcac tacgtcgccc aaggcgatcg ttcgctgttg ccactcgaag
6241 tcttcgacaa tggatttacg acggtattcc actttccggg caatgtacgc ataccctcca
6301 tctacaccat caatcctgat ggcaaggaag ctgttgccaa ctattcagtt aaagggagcg
6361 atgtcgagat ttcttcggtt tcccgaggtt ggcgtctgag ggatggccac acagtactat
6421 gtatctggaa caccgcttac gatcccgttg gccaaaggcc gcaaacgggc acggtgaggc
6481 ccgatgtgaa acgcgtcctg aagggggcga agggatgaat aacgatagtc agcaagcggc
6541 acatgaggtt gatgcatctg gatccctggt ctccgacaaa catcgccggc gtctttcggg
6601 gtctcagaaa ttgatcgtcg gaggtgtcgt tctcgcgtta tcattaagcc tcatttggct
6661 aggtgggcgc caaaagaagg tgaatgagaa cgcatcgccg tcaactttga tcgcaacaaa
6721 caccaagcca tttcatccag ctccgattga ggtgccgccg gatcctccag cggttcaaga
6781 ggctgttcag cctgctgctc ctctaccgcc gaggggcgaa ccggagcggc atgagccacg
6841 gccggaagaa acaccgattt ttgcatatag cagcggcgat caaggggtca gcaaacgcgc
6901 cattcagggc gacacgggcc gaagacaaga aggcaagcgt gacgacaact ccttgccgaa
6961 tggcgaagtg tccggcgaga acgatttgtc gatacgtatg aaacccaccg agctgcagcc
7021 cagcagcgcc acgctcttgc cgcaccccga ttttatggta acgcaaggga caataattcc
7081 gtgcatcttg caaaccgcaa tcgacacaaa tttggcaggc tatgtaaagt gtgtcttgcc
7141 tcaggatatt cgtggaacaa cgaacaatat cgtgcttctt gatcgtggca ccaccgttgt
7201 tggcgaaata cagcgtggct tgcaacaggg agatgggcgc gtttttgtgt tgtgggatcg
7261 cgccgagaca cctgaccatg cgatgatctc gttaacatcg ccaagcgcgg acgaactcgg
7321 tcgctcagga ttgccgggct cggtcgacag ccacttctgg cagcgtttta gcggagctat
7381 gctcttgagt gttgttcaag gcgccttcca ggcagctagc acctacgccg gcagctcggg
7441 tggcgggatg agcttcaaca gctttcaaaa taacggtgag cagacaactg agacagccct
7501 taaggcaacc atcaacatac cgccaaccct gaagaagaat cagggtgaca ccgtttccat
7561 tttcgtagca cgggacctcg atttctttgg tgtttaccag ctccgcctga ctggcggcgc
7621 cacgcggggg aggaaccgcc gctcttaatg aattcaaatt tccgcttaga gataggatac
7681 attgtaaatg gaagtggatc cgcaactacg ctttcttctg aagccgattt tggaatggct
7741 cgatgacccg aagactgaag aaattgcgat caatcgacct ggagaggcat ttgtgcgcca
7801 agccggcatt tttaccaaga tgcctttgcc cgtctcttat gatgatcttg aagatatcgc
7861 tattttagcg ggcgcgctga gaaagcagga tgtcggacca cgtaaccccc tctgcgccac
7921 tgaacttcct ggtggtgaac gactacaaat ctgtctgccg ccgaccgttc cctcgggcac
7981 cgtcagcttg accattcgac ggccaagctc gcgtgtttct ggtcttaaag aagtctcctc
8041 ccgttatgat gcttcgaggt ggaaccagtg gcagacacga aggaaacgcc aaaatcagga
8101 tgatgaagct atccttcagc attttgacaa cggggatttg gaagcgtttc tgcacgcatg
8161 cgtcgtcagc cgactgacga tgttgctatg tggccctacc ggaagcggca agacaacaat
8221 gagcaagacc ttgatcagcg ccatcccccc ccaggaaagg ctaatcacca tagaagatac
8281 gctcgaactc gtcattccac acgataatca tgttagacta ctctactcca agaacggtgc
8341 tgggctgggt gctgtgagcg ccgagcactt gctccaagca agtctgcgta tgcggccgga
8401 ccggatattg cttggcgaga tgcgcgacga tgcagcatgg gcttatctga gtgaagtcgt
8461 ctcgggacat ccgggatcga tttcaacaat acacggcgcg aatccaatcc aaggattcaa
8521 gaaactgttt tcccttgtca aaagtagcgc ccaaggtgct agcttggaag atcgcacact
8581 gattgacatg ctctctacgg cgatcgatgt catcattcca ttccgtgcct atgaggacgt
8641 ttatgaagta ggcgagatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg
8701 cgatctcctt aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga
8761 gaacttttgt cataaaattg aaatacttgg ttcgcatttt cgtcatccgc ggtcagccgc
8821 aattctgacg aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc
8881 tcaagcgctg tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt
8941 cttcttatcg atgacgatgt cgctatgcgg catcttatta tcgaatacct tacgatccac
9001 gccttcaaag tgaccgcggt agccgacagc acccagttca ctagagtact ctcttccgcg
9061 acggtcgatg tcgtggttgt tgatctaaat ttaggtcgtg aagatgggct tgagatcgtt
9121 cgaaatctgg cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag
9181 gagacggata aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttt
9241 agtacgagag agtttcttgc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc
9301 cgctccaaag accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt
9361 cgcttgatgt ccgaagctgg cggtgaggtg aaacttacgg ctggagagtt caatcttctc
9421 ctcgcgtttt tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt
9481 cgagtacgcg acgaggaggt ttacgacagg agtatagatg ttctcatttt gcggctgcgc
9541 cgcaaacttg aggcggatcc gtcaagccct caactgataa aaacagcaag aggtgccggt
9601 tatttctttg acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat
9661 tgcatttggc tcttaattat ctggctcaaa aggtgactga ggacgcggcc agcggcctca
9721 aacctacact caatatttgg tgaggggttc cgataggtcc ctcttcacca attgctcgat
9781 ggcttctctc cagcaaagaa tgacgcgagc gcggcggtag ccagcttgtg gccgaaagct
9841 cgagcggtct ccaaccccaa cggatcaaaa tgacttcgag cgacctcgag caacgcaacc
9901 gggaacatgc gtgaggtctg aacgagaacg gatttttctg tagttgaagg gatcggataa
9961 cttttcgggg ccacgcgaaa tgatccatct gccagcatgc tttcgaaatc gtccaacgcg
10021 cgccttaaaa tcatttgtag cgacttcgag ggactgtatt gccgaacgag gttgtcatat
10081 gttttcgaca cttgaggcgc gggcggtcgc gctgaaagaa aaacctggag ctttttcggg
10141 gacggaggtg gactaagggc atccacagtt agcttaagtt gtcgatcggg actgtaaatg
10201 tgatcggcga cgagaggctc acgttgctgg tctttctcgt cggctttttc aggcaagtgc
10261 tggaggtcca gcttctgggg aacaagtgtc gggttgggat ggtggatctc gggtcgagca
10321 ccagcaagcc gccgtgcttc gccgaccgac aatgcgggct tgcgaattgc catcttcaag
10381 cctccaagat tttgctgatc agtttcgaaa tgaccacgac ttcctccatc gcaatccgaa
10441 gattcctctc tatgaggcgc atcgtcggat cagttcccgt gtttagtaat gtaagatgca
10501 acatgccgcg ttctttcatc gcggcaaatg catctctttc atgcatggga gacggtacaa
10561 ctggaaggct ctctagcgtc tctgacatcc tgcgttgcga tgttgtcaat cggccgaccg
10621 ggacgcgttg gcgcaaaaca gctgtaggaa ttgccaaatt ttcactcaac agcagctcga
10681 tgacgtagcg gtaggtagat agtgcctcat cgatgtcgag cggcgttagc atggtgggga
10741 tcagaagcag gtttgagcta gcgatgattg tgttgttgag ctcgctcgag ccgccacgcg
10801 tatcggccaa cgcataatca aatccttcga gctcggcatt ttcataggct gcttcaagaa
10861 ggggcatttc gtcggcggaa tagacttcac agcgaggatc ccaggtactg ctttgtaagg
10921 cgttttctct ccatcgcgtc agaggccggt tttcgtcggc atcaaagagg gccactcgtt
10981 taccgtcatt tgccaaagca gcgcaaaggc ccatgagtgc ggtggttttg ccagcacccc
11041 ctttgaaaga acaaaacgtc aaaagttgca tattctgatc ccgcctatcc tgtgaaaccg
11101 gagtgcattt gtatttttgt tcgtataaat gtttttgtga ttatcgatga gtaaaagcgt
11161 tgttacacta tttttatttc acattcgtta taagacaatt gcaaatgtag caagtatatt
11221 cagtattgac tgtaaatgta ctgttgattt catattgagc agggctagac ttccatccgt
11281 ctacccgggc acatttcgtg ctggagtatc cagaccttcc gctttctttg gaggaagcta
11341 tgtcaaaaca caccagagcc acgtcgagtg agactaccat caaccagcat cgatccctga
11401 aagttgaagg gttcaaggtc gtgagtgccc gtctgcgatc ggccgagtat gaaacctttt
11461 cctatcaagc gcgcctgctg ggactttcgg atagtatggc aattcgcgtt gcggtgcgcc
11521 gcatcggggg ctttctcgaa atagatgcag acacacgaga aaagatggaa gccatacttc
11581 agtccatcgg aatactctca agcaatgtat ccatgcttct atctgcctac gccgaagacc
11641 ctcgatcgga tctggaggct gtgcgagatg aacgtattgc ttttggcgag gctttcgccg
11701 ccctcgatgg actactgcgc tccattttgt ccgtatcccg gcgacggatc gacggtcgct
11761 cgttactgaa aggtgccttg tagcacttga ccacgcacct gacgggagaa aattggatgc
11821 ccgatcgcgc tcaagtaatc attcgcattg tgccaggagg tggaaccaag acccttcagc
11881 agataatcaa tcagctggag tacctgtccc gaaagggaaa gctggaactg cagcgttcag
11941 cccggcatct cgatattccc gttccgccgg atcaaatccg tgagcttgcc caaagctggg
12001 ttacggaggc cgggatttat gacgaaagtc agtcagacga tgacaggcaa caagacttaa
12061 caacacacat tattgtaagc ttccccgcag gtaccgacca aaccgcagct tatgaagcaa
12121 gccgggaatg ggcagccgag atgtttgggt caggatacgg gggtggccgc tataactatc
12181 tgacagccta ccacgtcgac cgcgatcatc cacatttaca tgtcgtggtc aatcgtcggg
12241 aacttctggg gcaggggtgg ctgaaaatat ccaggcgcca tccccagctg aattatgacg
12301 gcttacggaa aaagatggca gagatttcac ttcgtcacgg catagtcctg gatgcgactt
12361 cgcgagcaga aaggggaata gcagagcgac caatcacata tgctgaatat cgacgccttg
12421 agcggatgca ggctcaaaag attcaattcg aagatacaga ttttgatgag acctcgcctg
12481 aggaagatcg tcgggacctc agtcaatcgt tcgatccatt tcgatcggac gcatctgccg
12541 gcgaaccgga ccgtgcaacc cgacatgaca aacaaccgct tgaaccgcac gcccgtttcc
12601 aggagcccgc cggctccagc atcaaagccg acgcacggat ccgcgtacca ttggagagcg
12661 agcggggtgc ccaaccatcc gcgtccaaaa tccctgtaac tgggcatttc gggattgaga
12721 cttcgtatgt cgctgaagcc agcgtgccca aacaaagcgg caattccgat acttctcgcc
12781 cggtgactga cgttgccatg cacacagtcg agcgccagca gcgatcaaaa cgacgtcatg
12841 acgaggaggc aggtccgagc ggagcaaacc gtaaaagatt gaaggccgcg caagttgatt
12901 ccgaggcaaa tgtcggtgag cccgacggtc gcgatgacag caacaaggcg gctgatccgg
12961 tgtctgcttc catccgtacc gagcaaccgg aagcttctcc aacgtgtccg cgtgaccgtc
13021 acgatggaga attgggagaa cgcaaacgtg caagaggtaa tcgtcgcgac gatgggcgcg
13081 gggggaccta gagacaggaa ggaccgaata atggcaaatg gtcagttcac gatacgctct
13141 gctcgcccgg cctccgtcgg actgacaggc gaacggcgtg gagccgcatc cgcctctagc
13201 tctgcactgt ccaatgttca aagagatgtt agggataggc tgattccaac tagctcacca
13261 agattaccaa atgcagccat attgcgtgat tcctcgggaa gagcgtcgac tggtctgcgg
13321 tacatggcgg ctactcttca ttggtctgcg atcgcgccat tatcgctaat aaacagcaac
13381 gacctggctc cggccgctta tgactttgag acgcgaaata acgcaagaaa tgtgactgcc
13441 aaagtcggca gggcagtccc tgttcccaag caaggcgggc tcggcaaaac gctcgcaccc
13501 gtacccctta gtacacgtat atcaagggtc aattccgacc gaagactgcc cgctgacgca
13561 gaagaccgcc ctgaaacgcg cgacccccag aaaggacgtg gcagtcatgg tgcgacgcca
13621 accttacatg aaaagattgg aaccgcgttt gctcgaagat tgcgaaagca tacgtactat
13681 attgtttgca gttgctgcca gacccggagc gcgttgacga tgggtgcaaa gatttcggtg
13741 aagtcatgaa ctccagcaag acttcgcccc agcgtatgac cctgagcatc gtatgttcgc
13801 tggcagccgg tttttgtgcg gccagctgct atgtaacgtt ccgccggggc ttcaacggcg
13861 aagcgatgat gacgttcgac gttttcgctt tttggtatga gaccccgctt tacttgggtt
13921 atgccagcac cgtcttctgg cgtggtttat ctgttgtcat ctttacctcg ctgatcgttc
13981 tttcaagtca gctcatcata tcgctgcgca atcagaagca tcatgggaca gctcgttggg
14041 cagaaattgg cgaaatgcgg catgctggtt atctgcagcg ttacagtcgc atcaaggggc
14101 cgatctttgg aaagacatgt ggtccccttt ggttcggcag ttatttgacc aatggcgaac
14161 agccacacag tcttgtcgtc gcgccaacgc gtgctggcaa aggcgtcggc atcgtcattc
14221 caacgctgtt gaccttcaag ggctcggtaa tcgcccttga cgtcaaggga gaattgtttg
14281 aactgacgtc cagagcacgc aaagcgagcg gcgacgcagt tttcaagttc tcccccctag
14341 atcctgagcg gaagactcat tgttacaatc cggtcctgga tattgccgca cttccgcccg
14401 aacgccagtt cactgaaaca cgccgtctag ctgcgaacct tattacggct aagggaaagg
14461 gagcagaagg ctttattgac ggcgcacgtg acctgttcgt cgcgggaatc cttacctgca
14521 ttgagcgtgg cacaccaacg attggcgcgg tatatgacct atttgcgcag cctggcgaaa
14581 agtataagct ttttgcgcaa ctcgcggagg aaagcctaaa caaagaggct cagcgtatct
14641 tcgataatat ggcgggcaac gacacgaaaa ttctgacatc gtacacctct gtgctgggcg
14701 acggtggact gaacctgtgg gctgatccgc ttatcaaagc agcgacaagc cggtcagact
14761 tttccgttta cgatctccgg aggaagaaga cctgcattta tctttgtgtc agtcccaacg
14821 atctggaggt cttggcacca cttatgcgcc tgatgtttca gcagctcgtg tcaatcttgc
14881 agagatcgct gccaggtgaa gacgagtgcc atgaagtttt atttctcctc gacgaattca
14941 aacacctggg caagcttgag gccatagaga ccgcgatcac aaccatcgcc ggttacagag
15001 gccgctttat gtttattatt caaagtcttt cggccttgtc gggcacatac gatgacgcag
15061 gaaaacaaaa ctttctgagc aatactggcg tacaagtatt tatggccacg gctgatgacg
15121 aaactccaac ctacatctca aaagctatcg gcgaatatac gtttaaagcg cgttcgacct
15181 cttacagtca agccagaatg ttcgaccaca acatccagat ttctgatcaa ggtgcagccc
15241 ttttgcgccc cgaacaagtg cgcctgctag acgatcagag tgaaatcgtt ctcatcaaag
15301 ggcgacctcc actcaaatta cgaaaggtgc agtattattc cgatcgtacg ctgaaaggcc
15361 ttttcgaacg ccagatgggc tctctgcctg agcccgcacc cttgatgctt tccgactata
15421 gcaacgatca agttcaatac cacttggctc cgatagcaaa ttttaatgag gatgctgcac
15481 cgcaaaacag aactgtggcc gaggaccatg gaagtgttaa agtcggtgct gatatccctg
15541 aacgcgtgat gggaataaat ggtgacgagg aacaagccga tgcgggcgag ataccgccgg
15601 aatcggttgt gcctccagaa ttgacgctcg ctctgaccgc tcaacagcaa ttgttggacc
15661 agattattgc acttcagcaa agatcgaggt ccgcaccggc atagcctgcg aaatgatctt
15721 aatggtgcaa ttcgtttcag gcggcatgtt gcggttcaac aaagtgtacg ccagatccca
15781 attggccctt tacgaggtgt cggtatgaca ggaaagtcga aagttcacat aagaggttcg
15841 gctgacgcgc ttcctgacgt tcctggcgga agtactaccg cccctttttt aaccgaacct
15901 tctcgggatc aggttgatgc ctcgtttgag gtccaaaccg actacagcca gtctacttcc
15961 gtgtcgttta cctatgatgg tgttggactt ggtcctgccg agcgtgcggc ttacgagaac
16021 tggtgcgaac cgggccggcc cacttggaaa gatcttataa tcaaggcacg tgtcgatccg
16081 attgacgatg tgacctggct ccgagattta gaagaggaca ccccctcaac cttcagatac
16141 gaagggatgc ctctgggcat cggggaacga caggcctacg aaaattggca agaggacgct
16201 cagccgacat gggaagacct tgttgtcagc gcacgcttga cggaacttgg ccgtccacac
16261 gggattaccg gcgagtatac atccctcgca ggatcgaaga atacaagttc aatttcattg
16321 aagcgaaagc ggagcaactt aattgatgat gagaattcat ccggatcgtt ttcatatgac
16381 gggatgaagc tcggggaagc cgagcgttct gcatatggtg actgggccga ggcggagcca
16441 cccacgtgga aagatttggt attgagggca cgcgtttcct cgatcaatga ctctgcttgg
16501 ctttttgatt cacaaacatc ttcatcatca tttgaataca acggtgttcc cttgggcgag
16561 ccggaacggc aggctctcag acaatggcaa ggagacgctc agcctacctg ggaagatctc
16621 gttgttaacg cgcgtatggc agaactttgc catgctggtt ggattgaagg tcaaaaaggt
16681 tgctttgaag agcgcgggga ggctctgccc gcgtcggaac gcggttcgca acgccccatt
16741 ggtcaacgga cagattcctc cgattctttt gtgtatgatg gcacaaggct cggagcacct
16801 gagcgaactg cttatgaacg ctggagtaag agggaacgcc cgacttggga agatctcatc
16861 ttagatgcac accaggccag gactgaaagt gacgctgtta cgacccaagc gattggtcag
16921 tcgtcctcac cggttttctt atatgaagga aagtcgctcg gagacaggga acgaaaggct
16981 tacgaaaaat ggcggcagcc agcccaaccg cgatggcaaa atcttgtagt gaacgctcgt
17041 ctggcagaaa tcgatccctc agcctggatt gccgatgagc gcgatccgct tgatgatagc
17101 gacgcgcttg gtcgcccgtc gtacacaagc ttgacggata gatcagacgt ccctttagac
17161 gatcaatcaa tctatcgtcg ttccgaccta gtaagggaac aagtaccaga atcgtctcaa
17221 aggcaattcg cagcatgttc agaatctgaa acgaggcctg tgcaatggtt tactgcttct
17281 gggtcagatg caaacaatac ggaaaatatc accgccagcg atcccgtcga tcgcacgggt
17341 ggagttaagc ggctaggctc caaaagcgac agaaccgtta cagcttctat ccatgacgtg
17401 aattccagca caaggcgact gttgcttaac gaatttggat cggaggctcc gcgcccttcg
17461 ccagaaaaga ctgttcgctt aagaagcgac aatattggca cctatgggag ccggaaaaat
17521 gaacgagcgc ggctcgcgac cgaaaccggt gcgtatgagt cggagcatat tttcgggttc
17581 aaggctgtcc acgatactgc gagagcgacg aaagagggcc ggcgtctcga aaggcccatg
17641 cccgcctacc ttgaggataa ggggcttcat cgccaacata ttggcaccgg gagaggacgg
17701 accaaacttg tcgggcgcgg atggccggat gacacaagct atcgctcgga tcaaagggca
17761 actctgtcgg accccgttgc gcgctcggaa ggcgcgacgg cctcaaatgg gtatcaattg
17821 aaccaattgg gttacgcgca ccaactcgct agcgatggcc tgcaaagtga atcgcccgat
17881 ggtgttgcct tgccaattca agtggcaaca acgagctaca actatacagt gagccgcgat
17941 cctgtccttg ttccgccgga taaaaacgaa gcccctcaat tgctgcatct tggtccccgt
18001 ggtcaaaccg aagctgttct tgcccgcgaa acagcattga ctggaaaatg gccgactctc
18061 gagcgtgagc agcaagtgta tcgcgagttt ttggccttat atgacgtaaa aaaagatctt
18121 gaggccaaat cagtcggcgt aagacggaaa aaaaaagaag ttatttctgc gttagaccga
18181 actgcgcgct tgataagcac gtcgccttcg aaagctcgat ccaaagcaga gactgaaaaa
18241 gccattgatg agctcgatga tcgacgagtt tatgatccgc gtgatcgagc tcaagacaaa
18301 gcgtttaaac gctgataagt cgccaatata gtgatcattt gcagtattcg catcgatcgc
18361 tggttgatat tctgccgctg gtcgaccggc tgctcgtcgc caaaaatgct cacagggata
18421 cgatggcctc ggtcaggccg cgtgcgtcct gtctttccag ttcctccctt tcagctcgat
18481 tgtggcatca tttattgcct gctcattgca gttgaaacgc gatatccgtt tcaagacccg
18541 ggtatggatg gtactttgga gatatgagca cttgccagac ttcccggtcg gagacttaaa
18601 aattccgata agaggttacg gacgacggac cttataagtg gatcgtctag tggtggcgcc
18661 gatcaaaaca gttccgcccc cggctttgct caaagtagca aagcagcttt atctcgggtt
18721 gcggaggatt ttctagaaaa ccgcaatttt gcaggagaga acatatggcc atcatcaagc
18781 cgcatgtgaa caaaaatagg acaacctcgc cgatagaacg tccggagtct ctcatagagg
18841 aaatgagcgg cagtcatccg ccgagtggtt ttaccaacct ggatctcgct atgatcgagc
18901 tggaggactt tgtccatcgg tgcccgctcc cagaagacaa tcttgctggt cagaaggagt
18961 gagacgatgg atccgtctag caatgagaat gtctatgtgg gtcgcggtca caacatcgaa
19021 aatgatgatg acactgaccc caggcgttgg aagaaggcga atatcagttc caacaccatc
19081 tccgatattc agatgacgaa tggcgaagac gtacaatcag ggagccctac ccgaacggaa
19141 gttgtaagcc cacgtctgga ttatggatcg gtcgactcct cctccagcct ttattctggc
19201 agcgagcacg gaaatcaagc tgagattcaa aaagagctgt ccgtcttgtt ctcgaacatg
19261 tctttgccag gcaacgatcg gcgcccggac gaatacattc tcgtgcatca aacgggacaa
19321 gatgctttta ctggtattgc caaaggcaac ctcgaccaaa tgcccaccaa ggcggaattt
19381 aacgcgtgct gccgtctcta cagggacgga gccggtaatt actacccgcc acctctcgca
19441 ttcgacaaga ttagcgttcc agagcaactg gaggaaaaat gggggatgat ggaggcgaag
19501 gaacgtaaca aactgcggtt tcagtacaag ttggacgtat ggaatcatgc gcacgctgat
19561 atggggatca cgggcacaga gatcttttat caaacagata agaacataaa gctcgaccgg
19621 aattataaac taagacctga agaccgatac gtacaaacag aaaaatacgg gcgccgggaa
19681 attcaaaagc gatatcaaca cgaactccag gctggttcgc tgctgcccga tattatgatc
19741 aaaactcccc aaaatgacat ccacttcgtg tacaggtttg ccggcgacaa ttacgccaac
19801 aaacagttca gcgagtttga acacaccgtc aagcgcaggt atggcgacga gactgagatc
19861 aaattgaagt caaagtcagg cattatgcat gactcgaaat atctggaatc ctgggaacgg
19921 ggcagtgcgg atattcgctt cgcggaattc gttggggaaa atagagctca caatcggcag
19981 tttccaactg cgacagtaaa tatgggacag cagccagacg ggcagggcgg tttgacccgc
20041 gaccgtcatg tgagcgttga cttcctaatg caaagcgcac ccaattcgcc ttgggcgcaa
20101 gctttgaaaa agggagaact gtgggatcgc gttcagttgc ttgctcgcga cggcaaccgc
20161 tatctgtcgc cgcccagatt ggaatattct gaccctgcac atttcaccga gttgatgaac
20221 cgggttggtt tacccgcatc gatgggtcgg caaagccatg cggctagtat caaattcgaa
20281 aagtttgacg cgcaggcagc ggttattgtc ttaaatggcc cagagttacg tgacattcat
20341 gacttgtctc ctgaaaaact gcaaaatttg tccaccaaag atgtcatcgt cgccgatcgc
20401 aatgagaatg gtcagagaac tggcacgtac accagcgtcg cggaatatga gcgcttgcag
20461 ttaaggctgc cacccgatgc agcgggggtg cttggtgaag caactgacaa atattcacgt
20521 gatttcgttc ggccagagcc ggcgtcgcgt ccaatcagtg acagccgcag gatatacgaa
20581 agtcgaccgc gtagccaaag cgtcaacagc ttttgacgtt cctgctgccg cgtcaacgag
20641 gaagctcgtt tgacccgggt ttgccaatga aagggctcaa tcatggtgaa cactacaaag
20701 aaaagttttg cgaagtcgct tacggcagat atgcgccgtt ctgctcagcg cgttgtcgag
20761 caaatgcgaa aagcattgat taccgaagaa gaggcgctca agcggcaagc cagactggag
20821 agtcccgata ggaagcgaaa gtatgctgct gatatggcga tagtcgacaa actcgacgta
20881 gggtttcgag gcgaaatagg ctataaaatt cttggaaata accggcttcg agtagacaac
20941 cataaagaat taacgcgtga gcacggtaga cttcgcaaaa ccaaaacggt tctgaagcgt
21001 aacccggtga cgcaggaagt ctatttgggt ttatatgaaa ggaagtcctg gttaagtgtc
21061 agcagccatt tgtatgctgc ggacggcaca ctccgcatga agcacgtgaa atacaaagac
21121 ggacgttttg aggaaaaatg ggagcgcgac gaaaatggcg acctgatccg cacaaggtac
21181 gccaaccgtg gcaggctctt tcaacctgta tccgagaaaa tgggcgcgcc gtatcggagc
21241 ggccctgacg accggctcta tcgcgatcta acccgtcgaa acggtttcag acgggagaca
21301 ttcgaacggg acgatcacgg aaacctcgag cgtatcggca gcaaccatgt cggcttttcc
21361 aagatttcag tgaaggcacc caatcgtcaa acctcccaga cgaagattca aaaacttggt
21421 ggcgctttca acaaatcttt taggtccctt ctggacaagg agggcaatga aatgggccgc
21481 gatattttga gccatcgacg gctctataac aagcggtctg ctgtctacga tgaagctacc
21541 ggacaattga agagtgccaa gcataccttc ggcaagatct acaggagcga aaccgaatat
21601 ctcagcgcgg gcctcaagaa ggtttcaaaa aagatactcg gggtgacggt ctaccggaaa
21661 tttgcggcgc tcagcgagcg agaatccgag gctgagagac tgcgtagttt tgaatccggt
21721 gcgcatcgcc agatctggca ggagcgggca gcgattcccg gttcgcccct cccggagact
21781 gatgacattc atttcgcaca gcagtcgcac ctagccaaag ccaaccctga tcacgtcgaa
21841 gctgacgtca cgcgtgtgac agatcaacat gttgatgttg ctggacaaac atcatcgtct
21901 ccccaacgga acttggaagg atggttagat tctcaatcac gatacaagcc agcaaacatg
21961 ctgttgtcaa atccagacct tcaagcgaac ggacctcgcc catacgaagg gttagctcat
22021 ctcaccctcc ggcgcgataa tgaatctgac gggcacaagg agaatgatca gcggctgcga
22081 catttctccc agccagagcc gttggtgtta ccgcatcccg ggtcgccgga aataactaag
22141 gtgtttggct cgcggggaga gccggcacac ccgagtggaa atctgcacac ggcggttgga
22201 gaaacggctt gcgaaggacc ggtgatgtct tcatcctcgg acaatcatca gccagctcca
22261 ggacagcaag aacttttaag tttccttcat aatgcgccag ccccagtttc tgtggcaata
22321 catgatgatc aagagcgact tgcgggggag gcgcccggcg gctctttcag aggtagctca
22381 gggcgaacga gttcaatgtc ggagagtatc ttcgacgaag atgtacaagg gcatttggta
22441 cgggattatt cgatcaatac tactaacggg tttattgacc cgcaatcgtt gttcggtgaa
22501 ccggacttat cgagaggtcc aaaatcgggg ccagaaattc catcggaaga ttaccatttg
22561 tcagcttcgg aacaggaaaa tttgctgaat caattgctta gtgtgccact gccggttcct
22621 tcaccgaagc ccgaatgcgc gaggtctatg attttcgaag gttcacgttc aagagagcgt
22681 tccacctcca gagggttcta aggtggaacg cgtctaggct tgcctatcat caccacctca
22741 tgatggtcgc ggcttaggga taacagggta ataagcttgg acctaaagtc gttaattaag
22801 ctatagtgca gtcggcttct gactagatag ctttctgagc cgccgatttt cctcctcgag
22861 ttggatgaac tcgccgagtt catcgtcaac tgaaacagac acggccggat tctgtgagac
22921 aggttgaacc gcagctctct tccattgata ataggtctga acggaaatac ccacgatctt
22981 aacggcgtcc ttcaaggttg cgccgccagc gacctgagct tcgatttgac cgatcttctc
23041 cagtttttct cggttgctga ggccgcgcgt tttcggcttc acggatttga acgatcccgt
23101 gcgggctgtt tcggctggtg ctttctttgc tcttctacct ctaggagcag ccggctcaac
23161 ttcggcagca gcagtaccgt ccggcggatt ctggatctct tcgtcagcca ttaatcgtcc
23221 tctgtgtggg ttattgcttt gtctgccagc tcgatccaag agtcaacgtt tgtgcctagc
23281 gcagtaaata ggcagtgctc cgcgactaca tgcctcggcc ggcaaaatac cgccgcatgt
23341 agagcaggct ctccttcacg atcaacgatc ggcatggggc cttcgtgctt gttgagtaat
23401 gttatcgctc ccatcagagc acgcttggta ctccgggaat cggatggtct gtcgatcatc
23461 caaaaaacgc tcatgttttc aacctattag gtctgtggtc agctgaccac agaccatcct
23521 gctccatact cgctaattct agccaaaccg caacgtcccc tgcccgctag ccttcaagag
23581 cgccattatc atcgggccaa gtgaaaactt cccgagttcg ctccgccgtg tcagatctcg
23641 gagatagccc ccaggcgaat tgatgaagtt cgctcgctcc aaaatgcacg ccatcgctgc
23701 tgccgcattc tccggtccca ttgcctcaca cgcgtcttgg taagccgacg ggctgacccc
23761 cagcatagac cgaaccacca ccgcagccga catgaggtca cgccagctag caaccgcacc
23821 gctcggccca taattgccaa tggtcgggca cgctttcagg atcatcccga gggggaacgc
23881 ttttatcggc tcgctccttg cccggtctat ttcactcggc ttagcgccct gctccttttc
23941 agagcgaggt tcaagttcat taacggattc gggttttgag ttctgtatgt gctgctcgct
24001 ctgggcagca ttggtgctat tattttctga attgtctcta atttccaacc ggttgattat
24061 ctcttcctgg agcatccaca tctcttcgag aattgactct acatcagcaa gcgtcggggc
24121 gcgtgggatt ctacccacaa gttccacata gacttcctcg acagcttgcc agtcgccctc
24181 cgctccctct tccatagctg ccgtaattag cttccgaacg tcccgtcggc aaatcgtcag
24241 actttctttg gccatcctga atgctgctcg atcggccatg acctgctgtg ccatcatcgc
24301 tagctcttcg gaccgcgcga gaagcggaga caaatcgaag ccaaacgcgc gctcgatctg
24361 accagcgcca tccttacgag cgtaacgctt tccgttggcg ctatccttcc ggacgatcaa
24421 gcctgactcc acgagcatgg cgatgtgcct acgcaaagtc gcgccagcca tcccatgcgc
24481 ccgaagggca agctgagcat tcgacgggaa gacgatcagc tgtgcctcct gacgcaactc
24541 cgtttccggg tgaaagctca atagcgcatc aaggacggca agactgttgg actggattcc
24601 aagtagttcc atcgccgcgc tcgcgtccct aaagaccttc cacttgtccg ctgtcttgcc
24661 ttgtttgata tcggccagcg ccgtctggcg ccgcacaagc gcaagcgtca ttggccgccg
24721 cccgaatggc gtcgttacac ttcctgtctg catcatcttt cacctttcag caggcaaagg
24781 aaatcagctc accaaaacgg cgctaaaaac tcttgacgag gattcgagga aatgcgattc
24841 tgttcgcgct agagagacag aagggcttcc gcgacggcga cgttgagggg gctcttttct
24901 tttgcggttt actctccccg tttccgttgg ttctcagcgt ggtacgcttg atacagcgct
24961 ggcacatgat cgagcacgaa ggtcgcaaaa tcgggcgtcg ccttcctgtc aatcgtgatt
25021 tccagtttgg ccttgctctg cgtcacctgt gcaattctgg tgccgtctgg ggtggccatg
25081 acctcgggaa gtccacgcgc aacccgactg ggcttcagac tagcgatcac cgccttgaat
25141 cgttctgccg atggcagcgc ttgaacttcc tccgacatag catatttagc cacgtcggcc
25201 ggtgaagaaa ctttctcaat cagctcggca agttgttgcc aactcggccg tccaacacca
25261 ggagcggcac caatagcatc ggtcagttca gaggggaggg cgtcaacgag cagaagcatc
25321 ttggacaaat tgctcttgtc gatcgacatc gcggcgatga caatctctcg agaaaactgc
25381 ctgttcaggc gatgtgcgaa gcgcgccttt tcgatgaagg taagatcttc gcgcacattg
25441 ttttcctgac cctgtgctac gaccacttgc tcgtccgtca gttcgcgaac gaccgccctg
25501 accggaagtc cgagttctga aacggcgcgt agccggcggt ggccgaaggc aacctgatat
25561 cggcccggct ggctcggatg cggtcgcaca aggattggga cttgctgtcc ttgttcccgg
25621 atcgaagtaa ggagcccgtc aatgtcccct cgcatacgat cctgcacgaa agacggttct
25681 attgacgagg catccaactc tatcactgcc tgaccttcag cgagacgccg ctcgatctct
25741 tcggcacggc taagacgatc gttttgctct cgcagtgcgt taccaatgtt cgctgtgagc
25801 ttcgttgccg gatcgcgctc cttccttgtt acgccgagga gcggcatgga gcggttcttt
25861 gccgtcctat tgtcggcggg cgacgtctca ggggcgtcag ttgagacgcc aaggatgtgc
25921 ttccggctca tgtgggccta ccccatgctt ttttgatcag tgtttcgatc tcgtcgttga
25981 cggcgttcat cgcctccaag gctcgatcat aggtcgagcg cgtgaacagg ccacgctcca
26041 cttcgaatag agtctggttt gtcaggccag cgtccgaaac ggcggtggtt ttaagcatcg
26101 gaaaattgag gacattttcg ccaaaaatcg accgcagata acctaccatt tggttctgtg
26161 gtccgtcgct cggttcgaaa cgggttatca gatagcgcat ccaattaaac ttgaacttgg
26221 cgccagcatt ctcgatttca cgcaaaaggt tcgatgtcat tgccagaaac tggttcatcg
26281 acatcacatc cagcatctgc ggatggaccg tgacaagaat ggacgtcgcc gcagtcaatg
26341 cggatagcgt gagataccca agctggggag ggcagtcgat gaccacgacg tcatagttat
26401 ccgcgatatc ttcaattact tggctgatgc gaccataaaa gagcgtgtcg ccctctttgc
26461 ggttcatcag cgcgcgtggc gtatcgtgtt caaactccat cagctcaagg ttaccaggaa
26521 tcaggtggag gtcgggaatg taagtccctc ggacgactcg ttcgattgcg acctgctcat
26581 catcatacct tatagcgccg tagagcgttt cgttcgggcc aacgtccgtc tccggttggc
26641 tcccaaagag tgcagaaagg ctcgcttgag gatcgagatc aatggccaag actcgatatc
26701 cgcgcatagc gaggtactgc gccagatgcg cggcggtggt ggtcttaccc gacccacctt
26761 tgaaattcat cacagagata acctgaagct gctcgccgcc tcgacgatgt ggcaggtagc
26821 gccggttccc acggccgacc tgatccatat acttccgaat cacatggata tcttcaattg
26881 agaacattcg cctgccacct gggctcatgc taacattcaa ctctggcatc tcagacgcgg
26941 tctgccgtaa atatgactcg ccaacgccga gcagcttgga cgcctccgat ggcccgaatg
27001 ttcgaatacc cttctcggaa tgcggcggga aaaccttaag atgatgtgct tgaagttggc
27061 tcgagagggc atcggcatga cgctccatca aggccgtcaa ccctacaact acaggcgctg
27121 cttttaggac agacttcgcc atctcaaacc cattccttgc cagtggcgat atttttcgcg
27181 aaactggaaa agttccgccg ctggcaatta gcgccgattc tgctgtttgg gcaagagctt
27241 ttaggttaac agaaggttaa cgccctcagg tcgaaaaact ccacccaact gttatttgta
27301 tttatttcca atgccttaga gagattgcca tttgaatatg ttcatgtatt gttttagtga
27361 taatcctaca atcgtaaccc aaaaagaggt cgccctctgc gcgccgtcgt ccaatatagg
27421 cgaagtcacc cttgcgactc aggcggattc taccttgtag gatcgtcgag ctgcctcgcg
27481 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
27541 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
27601 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
27661 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
27721 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
27781 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
27841 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
27901 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
27961 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
28021 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
28081 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
28141 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
28201 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
28261 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
28321 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
28381 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
28441 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
28501 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
28561 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata tctcccaatt
28621 tgtgtaggcg cgccttgggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
28681 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
28741 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
28801 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
28861 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
28921 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
28981 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
29041 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
29101 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
29161 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
29221 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
29281 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
29341 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
29401 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
29461 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
29521 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
29581 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
29641 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
29701 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
29761 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
29821 gaacaggctt atgtccactg ggttcgaatt cattaccctg ttatccctaa tgcagagcca
29881 ccccatcagg aatatcgccc attccgtcat cagcgtggtg acgtgcatca ggcgcgcttg
29941 ttgcagaact ttaagtgctt ctttcgattc gagatctcag caaaagttct ccgcaaacct
30001 tcaccgcggg cttacgtgtc ttttgtaaca aattgcgacg cagttgatat ccacttgaaa
30061 cattagtccg aaattatcga gatctccgct gataaggtat cgaaatggcg ataaaattgg
30121 tattgatact cgtattcaca ctctttcccg cggcagacgc tgcatatgcg aatgaccgcg
30181 ccaacggttt catgtggtca aacgggggcg aaactggagt gaggcttcct cttcgggtgt
30241 tcaatgccaa gccagccaag aacacggtgg cgatcattta ttccggagac gctggatggc
30301 aaaatatcga tgaggcgatt ggtacctatc tgcagacgga agggattcct gtcattggcg
30361 tcagttcact tcggtatttc tggtcggagc ggtccccaag cgaaactgct aaggatcttg
30421 gtcacataat cgatgtctac accaagcatt tcggtgtgca gaatgtttta cttgtaggat
30481 attctttcgg cgcggacgtc atgccggcaa gcttcaatag gcttacgctt gagcaaaaaa
30541 atcgggttaa gcaaatctct ctcttggcat tgtcacatca agtcgactat gtcgtctcat
30601 ttaggggctg gctccaactc gaaacggaag gtaagggcgg caatcctctg gatgatctca
30661 gatccattga ccctgcaatc gtccaatgca tgtacgggcg cgaagaccgt aataatgctt
30721 gcccatctct ccgacagacc ggcgcagagg tgataggctt cagcggaggc catcactttg
30781 ataatgattt caaaaaactg tctacgcgcg tcgtctcagg cctcgtggca cgcctaagtc
30841 atcagtaatc tttagttcct gcaccgcttt agtattgact gggatagcga cgcctgtgat
30901 gcagacatcg gatattgtgt cgttaagtaa aaggccttcg tctgatcgcg agactcgcta
30961 gtggttttca ggtgagtgag atgttttgcc gcaagttgcg ctgagatcgc atctgcctgc
31021 ggctgccgca cctccagatt ggcagcaaca agatcatcct tcaagggaag atgcctataa
31081 cgcatgtgtt gacgactttc gcctcgtgaa tgatccggtc tgttcccgac agtgggatgc
31141 cgtcgcgata gagcaggctc tcgcgccagg aggaatttct ccgatatgac ttctctcctt
31201 ctacgtgccc aaagcagcaa cgaaatcatc tatcgcggcc aaaagagcgg tcatgtgaaa
31261 gcacccttgc cagtcaattc cctggccaag gtcagcacgg cgatactgcg aggtgttgct
31321 acaccacgga ggcgatctag aaggacgatt tcatcattta ggccgcacac tcataaatca
31381 ggtttgcaaa tcggtctgat tttgattcat tgaggcttga cttggaggcc actgataacc
31441 cgtccccgct ttgatgtcac cgatttcgaa tggactgttg ttcagtcctt tgttcccaaa
31501 taagccgcgt cgcgtgccgc gcgttgacgc gggtgatcaa tgtcatcttg tggcgcttcc
31561 ggacgggccc gccctgggca gacgttcctg atcgatacgg ttcctgtaca acctgctaca
31621 atcgctttgt acgatggcgt gaggcagacg tctgggatca tattctgagt gtgatttcca
31681 aggctttcga tagaatgtcg tcatgattga cagttcctgt gtccgcgtcc accaacatgc
31741 ggcgacggga aacagcggga tcaagacgat ggctgtatgg gacgttcccg tggcggtttg
31801 accaccaaga ttcacgctgt tgtcgatgcc gacggttgac cgatccgtct tgcgctgaca
31861 gcgggtcaag cccatgaccg ccgcatgacc gaaacatttt tacaaataat cgcttagagt
31921 gcgtttctgc tggcggacaa ggcatatgat accaacgcga taagagcatt tgcaaagcgc
31981 aagcaggcat gggccaatat tcctgccaag agcaatcgga aggaagcttc ccgttcatcg
32041 aatgggttta cagacagcgt aacctcgccg agcattttcg ttctgagcct gacctgacca
32101 actgcggcct gaataggtcg atccggttgc ttagtcatgg atgcgcggtt ctcggtccat
32161 gttgcgttcc aagacgccgg gcgaggtttc tcgcttcaat tgaaatcata aagaagcaat
32221 tgaaaatttt cgagtaaccg accctcccga taatcttcaa cataaaacaa cgcacttctt
32281 ccaacgggag aggcggtgtt agttgcgagc taaggagata aggtatgctt aagagatcgg
32341 ggtcgctttc tcttgccttg atggtgagct tctgttcgtc gagccttgcc acgccactct
32401 catctgctga gtttgaccat gttgctcgca agtgtgcccc atcagttgcg acatctacgc
32461 ttgcggcgat agctaaggtg gagagtcgct ttgatccttt agcgattcat gacaacacga
32521 ccggcgaaac gcttcactgg caagatcaca gccaagcaac ccaagtcgtc aggcgccgtc
32581 tcgatgcacg gcattcgctg gatgttggcc tcatgcaaat aaactctcga aatttttcta
32641 tgctcggtct gacacctgac ggtgcgctcc aggcgtgcac atcattatct gccgctgcaa
32701 acatgctgaa aagtcgttat gcaggcggcg aaacgattga cgagaagcaa tttgcgcttc
32761 gtcgggcgat ctccgcttac aacaccggta atttcatcgg cggttttgca aacggctacg
32821 tgcgaaaagt tgaaacagct gctcaatcgc tggtgcccgc gttaatcgag cctccaaaag
32881 acgatcacga ggcgctaaaa tccgaagaga cgtgggatgt ttgggggtca tatcagcgcc
32941 gctcgcagga ggatggcgct ggcggtttaa tcgctccgcc accgccacac caggacaacg
33001 gcaaatccgc agacgacaat caagtcttat tcgacttata c
//
