pMT-DEST48

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价格：元

联系方式：I47-825O-882O

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pMT-DEST48载体质粒基本信息

出品公司:	Invitrigen
载体名称:	pMT-DEST48
质粒类型:	Gateway载体；昆虫细胞表达载体
高拷贝/低拷贝:	高拷贝
启动子:	MT
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5179bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	V5 Epitope Tag ; 6xHis Tag
载体抗性:	氨苄青霉素
筛选标记:	--
备注:	--
产品目录号:	12282-018
稳定性:	瞬表达
组成型:	组成型
病毒/非病毒:	杆状病毒

pMT-DEST48质粒图谱载体图谱和pMT-DEST48载体序列质粒序列多克隆位点信息

pMT-DEST48载体图谱

pMT-DEST48质粒载体简介

The pMT-DEST48 destination vector is designed for rapid cloning with a Gateway entry clone using lambda phage site-specific recombination and subsequent expression in Drosophila S2 cells. As part of the DES Expression System, pMT-DEST48 uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium. The pMT-DEST48 vector offers the following features:

 C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
 C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
 R sites for efficient recombination with any attL-flanked Gateway entry vector

pMT-DEST48质粒序列载体序列

TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCT
GTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGG
CTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGAT
GCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATC
GGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTA
ACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTAATTCGTTGCAGGACAGGAT
GTGGTGCCCGATGTGACTAGCTCTTTGCTGCAGGCCGTCCTATCCTCTGGTTCCGATAAGAGACCCAGAA
CTCCGGCCCCCCACCGCCCACCGCCACCCCCATACATATGTGGTACGCAAGTAAGAGTGCCTGCGCATGC
CCCATGTGCCCCACCAAGAGCTTTGCATCCCATACAAGTCCCCAAAGTGGAGAACCGAACCAATTCTTCG
CGGGCAGAACAAAAGCTTCTGCACACGTCTCCACTCGAATTTGGAGCCGGCCGGCGTGTGCAAAAGAGGT
GAATCGAACGAAAGACCCGTGTGTAAAGCCGCGTTTCCAAAATGTATAAAACCGAGAGCATCTGGCCAAT
GTGCATCAGTTGTGGTCAGCAGCAAAATCAAGTGAATCATCTCAGTGCAACTAAAGGGGGGATCTAGATC
GGGGTACATCAAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATAT
TAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCG
GCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGG
ATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGA
TATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAG
ACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCT
TTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCT
GGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTC
TGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTG
AAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAG
TTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAA
TATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCT
TCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATCTAG
AGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATA
TATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGT
TGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCA
TGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGT
CGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGT
TTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCC
GGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACC
CGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGT
TATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTC
TGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGT
TGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCAT
TTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTCGATGGTTCGAAGGTAAGCCTATCCCTAACCC
TCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCATCACCATCACCATTGAGTTTAAACCCGCTGATCA
GCCTCGACTGTGCCTTCTAAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG
AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGG
TTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCAGTCGACCTGCAGGCATGC
AAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAAC
ATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGT
TGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGC
GGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTT
CGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTT
TTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCG
ACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAG
GTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGAC
CGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCC
TAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAA
AGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTG
GAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTA
AATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCT
TAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGT
GTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGC
TCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAA
CTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAG
TTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTC
AGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCT
TCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCA
TAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTC
TGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATA
GCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCT
GTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGC
GTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTT
GAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATA
CATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCT
GACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGT

pLenti6/V5-GW/lacZ	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG
pAP1(PMA)-TA-Luc	pIEX-1
pHR31	pSuper.Retro.Neo
pGEX-6p-1-hsG3BP2b	FUW-tetO-hOCT4
pCRE-DD-AmCyan1	pSwitch
pHBT-GFP-NOS	pL-brevisGH1-TA
pGL4.21[luc2P/Puro]	pWY113 pET28a_AtmtE2
pSEAP2-Basic	pBABE-HAII
pSP65-F1+	pAAVLINK-EF1a-5'-PXDN
pAX01-ComK	pAAV-hSyn-Cepheid1s-WPRE
pBluescript II KS(-)	pAMβ1
Pgex-HRV3C	pAAV-EFS-DIO-GRAB_OT1.0
pAAV-TRE-CAG-Fluc	p1JI008
pcDNA3.1/Zeo(-)	pMCS-Green-Renilla-Luc
pQE-16	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG
pCRII-TOPO/22-1	pHIL-D2
pBTB-5	pCEP4-DsRed2
pET-53-DEST	pUTK21
pAAVLINK-EF1a-HA-5'-ITSN1	pUC19
pSW510	pHT01
pFN10A (ACT)	pLVX-AmCyan1-N1
pGFP22	pBBR1MCS2-Tac-mCherry
pET28a-mOrange(M163A)	pRetroX-Tight-Hyg
pAAVLINK-EF1a-HA-5-RALGAPB	pHisB-Sumo-NeMeHg
ppyrG	pDB262
pENTR5'/CMVp	pENTR-L1pLaclacZaL4
pS-hydro_TA	pNov.aro-bgl1-640
pHY300PLK	pVK101
pIRESpuro3	pcDNA4/TO/Myc-His/LacZ
pcDNA3.1+N-DYK-P2A	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG
pIEx/Bac-3	pCEP4-Flag-N
pYES2-EGFP	pGEX-6p-1-AT5G48650
pShuttle-CMV	pYC25
pPM408	pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG	pAAV-CAG-NES-YTnC
pIEX-3	pRI101-AN
pMAL-p5x	pDM4
pBARMTE1	pENTR U6
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG	pIZT/V5-His
pCold-SUMO	pEYFP-Mem
YEp24	pCDEF-HA-Ub-K63R
pcDNA3.1-CNTRFv1-HA-His	pQLinkN
pAAVLINK-EF1a-HA-5'-DIP2C	p19D+
pLC4-14	pDEST8
YEp16	pcDNA6.2/nTC-Tag-DEST
YEp620	pBad33
pMMB66EH	pDNAPolbeta_TA
pBR327	pFR-Luc
pHOOK1	pcDNA3.1-mRFP
pFastBac1-His-C	pGL4.18[luc2P/Neo]

pHCMV_DUGV_GP	pCAG-FAST-p10
pBiFC-VN155(I152L)	pCI-neo.mCherry-NEDD4
pJRH-1179 U6-reci Gag-Cas9 v2	pcDNA3.1-TYMS
pBK092	pKAR2-Br512
CD109-bio-His	pcDNA3.1_AktPH-mCherry
mARG1 cassette 3	pRK5-HA-SH3BP4 W92A
SIRT6 Flag	pMR-A-3
pKO (Hyg)	pET28GST-LIC
pHIV-Luciferase	pGEX6p-1-BCCP-PupE
pGHYB	LeGO-iG
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-	pXR002:EF1a-dCasRx-2A-EGFP
mAzurite-8xbiRhoBAST-24xMS2v6	AdEasier-1 cells (strain)
PX551	pT7-SpCas9_sgRNA-site1 (RTW443)
pEF1a-Usp9x_CD-mCherry	pCI-6.20
pEBTet_NAD-Snifit-mito	GFP-MAP1B
BCR/ABL P190 transgenic construct	pCDH-DCX-TurboID-V5-puro
pShuttle-CMV-GFP U6promoter	pRK5-HA-Ubiquitin-K29R
pCDNA3 Flag p38 alpha	pAc-sgRNA-Cas9
pscAAV-CAG-GFP	pQCasTns(Vch)-entry(BsaI)
GW1-Peredox-mCherry-NLS	pSI-Target-AID-NG
pGP-CMV-Gcamp6m	pMIH-TOMM20-Halo
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA	pAAV2/9n
MG1655 ΔendA ΔrecA (DE3)	pRH2502
phage UbiC G3BP1-SNAP	pDawn-sfGFP
pcDNA3.1-ERBB4	pET16B.Pfu
pQUAST>stop>mCD8-GFP	pMXs-hSOX2
pX330_SpCas9_USP7 Exon 3 gRNA	pEG302 22aa SunTag NtDRMcd (no NLS) g4+g
pCMT-flp	pCITEp65
MP6	Myc tag WT PTB
pPB-AMBRA1-3xFLAG	PA-GFPmt
pTM1_VP30_ZEBOV	pCMV-mA1-BE3
pCS2+HyPer7-NLS	pCRISPR-cBEST
pTA-HUH	pEGFP-C1 F-tractin-EGFP
pcDNA3 Connexin43-GFP-APEX2	pAKTaq
pcDNA3.1 SIRT6_H133Y	pBLO 64.1
murine TCR OTI-2A.pMIG II	pcDNA3.1(+) CircRNA Mini Vector
BB3cK_pGAP_23*_pTEF_Cas9	LentiGuide_DKO
pBIG1a	pBSK
pLTR-RD114A	pLenti-MP2
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3	EGFP-LC3
shYAP1	pINDUCER21-puro_Gateway-3xFLAG
pCMV-HA-JMJD3	pNG9
p6XHis-T7(P266L)	pDriver
GAG-CRErec	Fuw-dCas9-Dnmt3a_IM
pCutamp	pcDNA5-T/O-Ds1-mCherry
pLminP_Luc2P_RE4	CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGre
Double UP mNeon to mScarlet	pT7-hMLH1dn for IVT
CAMK2D gRNA (BRDN0001146112)	pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
PB533_15F11-sfGFP	pLIC-SM-HA
pQCXIP-GFP-LacR	pAAVS1-PC-GCaMP6f

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pMT-DEST48

买家导航

pMT-DEST48载体质粒基本信息

pMT-DEST48质粒图谱载体图谱和pMT-DEST48载体序列质粒序列多克隆位点信息

pMT-DEST48质粒载体简介

pMT-DEST48质粒序列载体序列

pMT-DEST48载体图谱质粒图谱pdf版和pMT-DEST48载体序列质粒序列等相关资料下载

pMT-DEST48质粒载体应用举例

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