| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-WNK3-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001559 |
| 产品用途: | 3' AAVLINK plasmid for WNK3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | WNK3 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-WNK3-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-WNK3-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-WNK3-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-WNK3-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-WNK3-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-WNK3-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-WNK3-FLAG 6554 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6554)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6554
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3267
/codon_start=1
/label=WNK3
/translation="GIQRPILLLCQAVEPFICRQEVDILA*ALLVLVSKILLARNPGLA
N*KAGHTGYGSQPAFSRDQKSVKWKQKR*DQQLLLIPFL*HGSPQLILGL*IDVKR*VD
HFSGVGSR*LQFLSSSQQK*HLLE*NTYQCSVRQTILVKKPLLKQQSLSW*K*NLPHKI
QKLRFLMRSYKLFRKPVKKIKEFPNKVTTSYLSAQLVRLMYLQ*PQKRNLKKLQPQEVA
CSLDLNCCLKRERY*LLGNSLALIVNFQPVLLAVESQWQRLVQRVISAYHTTKNKLMLK
HRVHSSIRHLPQ*AVMMNQK*RMRT*RWSFKDYEKNTFRRW*IFKPSRIRSCRSSMNAF
GQLKIAKPNLLRFLCHLHHHVDQDLSKANFEAAPSP*HMWTMA*LLQGKAAL*MSWKIH
CVWRVMQHHANSLQPVKKGCSQMTYTSWWMTGQRKQ*EILLLSQV*TNLNKVNTN*RQK
TGIKYLKILRLLWATHQHGFLLCPKSVELSQLPCHKDSHSLHFLGHYHHMECLTFVSIM
LWRGRGIQYSG*EFQEQHNNL**FPPNLGDHSSQG*ICRHFQLHQCRILPQSLLVL"
misc_feature 1605..1610
/label=SDS
misc_feature 2504..2509
/label=SDS
misc_feature 3253..3258
/label=SDS
CDS 3274..3339
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3343..3774
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3487..3492
/label=SDS
repeat_region 3817..3957
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3817..3946
/label=AAV2 ITR
rep_origin 4032..4487
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4769..4873
/gene="bla"
/label=AmpR promoter
CDS 4874..5734
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5905..6493
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggga tccagaggcc
1561 tatcctgctg ctgtgtcaag cggtggagcc attcatctgc agacaggagg tggatatttt
1621 ggcctaagct ttacttgtcc tagtctcaaa aatcctatta gcaagaaatc ctggactcgc
1681 aaattaaaaa gctgggcata caggctacgg cagtcaacca gctttttcaa gagatcaaaa
1741 gtccgtcaag tggaaacaga agagatgaga tcagcaattg ctcctgatcc cattcctctg
1801 acacgggagt ccacagctga tactagggct ttgaatagat gtaaagcgat gagtggatca
1861 tttcagcggg gtcggttcca ggtgattaca attcctcagc agcagtcagc aaaaatgaca
1921 tcttttggaa tagaacacat atcagtgttc agtgagacaa accattctag tgaagaagcc
1981 tttattaaaa cagcaaagtc tcagttggta gaaatagaac ctgccacaca aaatccaaaa
2041 acttcgtttt cttatgagaa gttacaagct cttcaggaaa cctgtaaaga aaataaagga
2101 gttcccaaac aaggtgacaa cttcttatct ttcagcgcag cttgtgagac tgatgtatct
2161 tcagtgaccc cagaaaagga atttgaagaa acttcagcca caggaagtag catgcagtct
2221 ggatctgaac tgttgcttaa agagagagag atattgactg ctgggaaaca gcctagctct
2281 gatagtgaat tttcagccag tcttgctggc agtggaaagt cagtggcaaa gactggtcca
2341 gagagtaatc agtgcttacc acaccacgaa gaacaagctt atgctcaaac acagagttca
2401 ctcttctatt cgccatcttc cccaatgagc agtgatgatg aatcagaaat agaggatgag
2461 gacttgaagg tggagcttca aagattacga gaaaaacaca ttcaggaggt ggtaaatctt
2521 caaacccagc agaataagga gctgcaggag ctctatgaac gccttcggtc aattaaagat
2581 agcaaaaccc aatctactga gattcctttg ccacctgcat caccacgtcg accaagatct
2641 ttcaaaagca aacttcgaag ccgcccccag tccttgacac atgtggacaa tggcatagtt
2701 gctacaggga aaagctgcct tataaatgag ttggaaaatc cactgtgtgt ggagagtaat
2761 gcagcatcat gccaacagtc tccagccagt aaaaaaggga tgttcacaga tgacttacac
2821 aagctggtgg atgactggac aaaggaagca gtaggaaatt ctcttattaa gccaagttta
2881 aaccaactta aacaaagtca acacaaacta gagacagaaa actggaataa agtatctgaa
2941 aatactccgt ctactatggg ctacacatca acatggattt cttctctgtc ccaaatccgt
3001 ggagctgtcc caacttcctt gccacaagga ctctcactcc cttcatttcc tgggccatta
3061 tcatcatatg gaatgcctca cgtttgtcag tataatgctg tggcgggggc ggggtatcca
3121 gtacagtggg taggaatttc aggaacaaca caacaatctg tagtaattcc cgcccaatct
3181 gggggaccat tccagccagg gatgaatatg caggcatttc caacttcatc agtgcagaat
3241 cctgccacaa tccctcctgg tcctaaatgt acagactaca aagaccatga cggtgattat
3301 aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc
3361 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
3421 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
3481 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
3541 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
3601 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
3661 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
3721 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct
3781 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
3841 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
3901 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
3961 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
4021 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
4081 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
4141 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
4201 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
4261 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4321 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
4381 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
4441 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
4501 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
4561 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
4621 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
4681 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
4741 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4801 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4861 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
4921 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
4981 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
5041 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
5101 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
5161 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
5221 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
5281 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
5341 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5401 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5461 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5521 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5581 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
5641 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
5701 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
5761 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
5821 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
5881 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
5941 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
6001 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
6061 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
6121 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
6181 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
6241 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6301 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6361 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6421 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6481 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6541 ttttgctcac atgt
//
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