pLR-NN

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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pLRNN载体基本信息

质粒名称: pLR-NN
出品公司: 爱迪基因
目录编号: 31017
存储实验室: Adam Bogdanove, Daniel Voytas

载体骨架基本信息
载体名称: pCS468
载体抗性: 大观霉素
空载体大小: 2460 bp
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: last NN repeat
物种来源: Xanthamonas oryzae
插入大小: 107 bp
克隆方法: 限制性内切酶
5' 克隆位点: ApaI(未破坏)
3' 克隆位点: XbaI (未破坏)
5' 测序引物 pTC14_F1 (cctactcaggagagcgttca)

文献引用方法
材料和方法部分: pLR-NN was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31017 ; http://n2t.net/addgene:31017 ; RRID:Addgene_31017)
参考文献部分: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687

pLRNN载体图谱质粒图谱和多克隆位点信息

pLR-NN质粒图谱

pLRNN载体简介

pLRNN载体序列

LOCUS       pLR-NN                2568 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLR-NN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Apr 14. ():.
  PUBMED    21493687
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             498..1289
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1382..1970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1871..1890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2124..2141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2300..2327
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2419..2505
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2554..8
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2568..17
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc ccgtctcgct atcgccagca acaatggcgg
       61 caagcaagcg ctcgaaagca ttgtggccca gctgagccgg cctgatccgg cgttggccgc
      121 gttgaccaac gagagacgtc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
      181 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
      241 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
      301 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
      361 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
      421 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
      481 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
      541 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
      601 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
      661 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
      721 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
      781 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
      841 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
      901 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
      961 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     1021 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     1081 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     1141 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     1201 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     1261 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     1321 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1501 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1741 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     1801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1981 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2041 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2101 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2161 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2221 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     2281 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     2341 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     2401 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     2461 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     2521 ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgtt
//

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