| 质粒名称: | mEos3.2-Clathrin-15 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 57452 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mEos3.2 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Clathrin,NM_001834.2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | mEos3.2 |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mEos3.2-Clathrin-15 was a gift from Michael Davidson (Addgene plasmid # 57452 ; http://n2t.net/addgene:57452 ; RRID:Addgene_57452) |
| 参考文献部分: | Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms. Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, Bewersdorf J. Nat Methods. 2013 Jul;10(7):653-8. doi: 10.1038/nmeth.2488. Epub 2013 May 26. 10.1038/nmeth.2488 PubMed 23708387 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mEos3.2-Clathrin-15 5313 bp ds-DNA circular SYN 23-SEP-2019
DEFINITION Localization: Clathrin Vesicles, Excitation: 507 / 572, Emission:
516 / 580.
ACCESSION .
VERSION .
KEYWORDS mEos3.2-Clathrin-15
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5313)
AUTHORS Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ,
Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D,
Bewersdorf J
TITLE Video-rate nanoscopy using sCMOS camera-specific single-molecule
localization algorithms.
JOURNAL Nat Methods. 2013 Jul;10(7):653-8. doi: 10.1038/nmeth.2488. Epub
2013 May 26.
PUBMED 23708387
REFERENCE 2 (bases 1 to 5313)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 23, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5313
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 94..397
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 398..601
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 552..572
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 646..1323
/codon_start=1
/product="improved monomeric variant of green-to-red
photoswitchable fluorescent protein EosFP (Zhang et al.,
2012)"
/label=mEos3.2
/translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
NARR"
polyA_signal 2134..2255
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2171..2190)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2225..2244
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2262..2717)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2399..2420)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2611..2630
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2744..2848
/gene="bla"
/label=AmpR promoter
promoter 2850..3207
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2873..2893)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3058..3193
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3120..3139
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3242..4036
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3296..3315)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3906..3925
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4224..4243)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4268..4315
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4644..5232
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5133..5152
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
61 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
121 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
181 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
241 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
301 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
361 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
421 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
481 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
541 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
601 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatgag tgcgattaag
661 ccagacatga agatcaaact ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc
721 gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa
781 gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac
841 agggtattcg ccaaatatcc agacaacata caagactatt ttaagcagtc gtttcctaag
901 gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcaa cgccagaaac
961 gacataacaa tggaagggga cactttctat aataaagttc gattttatgg taccaacttt
1021 cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa
1081 atgtatgtgc gtgatggagt gctgacgggt gatattgaga tggctttgtt gcttgaagga
1141 aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag
1201 ttaccaggcg cccactttgt ggaccactgc attgagattt taagccatga caaagattac
1261 aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga
1321 cgatccggac tcagatctcg agctcaagct tcgaattctg cagtcgacat ggctgatgac
1381 tttggcttct tctcgtcgtc ggagagtggt gccccggagg cggcggagga ggacccggcg
1441 gccgccttcc tggcccagca ggagagcgag attgcaggca tagagaacga cgagggcttc
1501 ggggcacctg ccggcagcca tgcggccccc gcacagccgg gccccacgag tggggctggt
1561 tctgaggaca tggggaccac agtcaatgga gatgtgtttc aggaggccaa cggtcctgct
1621 gatggctacg cagccattgc ccaggctgac aggctgaccc aggagcctga gagcatccgc
1681 aagtggcgag aggagcagag gaaacggctg caagagctgg atgctgcatc taaggtcacg
1741 gaacaggaat ggcgggagaa ggccaagaag gacctggagg agtggaacca gcgccagagt
1801 gaacaagtag agaagaacaa gatcaacaac cgggcatccg aggaggcttt cgtgaaggaa
1861 tccaaggagg agaccccagg cacagagtgg gagaaggtgg cccagctatg tgacttcaac
1921 cccaagagca gcaagcagtg caaagatgtg tcccgcctgc gctcggtgct catgtccctg
1981 aagcagacgc cactgtcccg ctagggatcc accggatcta gataactgat cataatcagc
2041 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
2101 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
2161 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
2221 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
2281 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
2341 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
2401 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
2461 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
2521 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
2581 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
2641 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
2701 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
2761 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
2821 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
2881 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
2941 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
3001 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
3061 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
3121 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
3181 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
3241 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
3301 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
3361 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
3421 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
3481 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
3541 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
3601 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
3661 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
3721 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
3781 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
3841 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
3901 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
3961 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
4021 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
4081 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
4141 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
4201 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
4261 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
4321 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
4381 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
4441 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
4501 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
4561 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
4621 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
4681 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
4741 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
4801 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
4861 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
4921 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
4981 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
5041 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
5101 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
5161 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
5221 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
5281 tttgctcaca tgttctttcc tgcgttatcc cct
//
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