| 质粒名称: | pHR_Gal4UAS_tBFP_PGK_mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79130 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | tBFP |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_Gal4UAS_tBFP_PGK_mCherry was a gift from Wendell Lim (Addgene plasmid # 79130 ; http://n2t.net/addgene:79130 ; RRID:Addgene_79130) |
| 参考文献部分: | Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28. 10.1016/j.cell.2016.01.012 PubMed 26830878 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_Gal4UAS_tBFP_PGK_mCherry 10765 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors
with a constitutive mCherry.
ACCESSION .
VERSION .
KEYWORDS pHR_Gal4UAS_tBFP_PGK_mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10765)
AUTHORS Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
WA
TITLE Engineering Customized Cell Sensing and Response Behaviors Using
Synthetic Notch Receptors.
JOURNAL Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
Epub 2016 Jan 28.
PUBMED 26830878
REFERENCE 2 (bases 1 to 10765)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10765
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 455..520
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 650..670
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 1095..1229
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1132..1151)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1186..1205
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 1397..2030
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 2077..2202
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 2699..2932
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 3117..3161
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 3446..3563
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 3789..3813
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 3894..4595
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
DLPSKLGHKLN"
promoter 4718..5217
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(4753..4776)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5150..5169
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 5238..5948
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(5383..5401)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 5651..5670
/label=mCherry-F
/note="mCherry, forward primer"
misc_feature 6025..6613
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6078..6098)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6496..6507)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6616..6632)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6617..6633)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6723..6956
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(6981..6999)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(6982..6999)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(7289..7308)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(7522..7541)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7641..7663
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7701..7719)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7787..7891
/gene="bla"
/label=AmpR promoter
CDS 7892..8752
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8110..8129)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8923..9511
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9412..9431
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9665..9682
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(9752..9772)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9757..10086
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9937..10072
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9999..10018
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
ORIGIN
1 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
61 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
121 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
181 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
241 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
301 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
361 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
421 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
481 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
541 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
601 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
661 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
721 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
781 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
841 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
901 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
961 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
1021 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
1081 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
1141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
1201 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
1261 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
1321 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
1381 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
1441 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
1501 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
1561 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
1621 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
1681 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
1741 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
1801 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
1861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
1921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
1981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
2041 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
2101 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
2161 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
2221 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
2281 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
2341 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
2401 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
2461 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
2521 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
2581 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
2641 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
2701 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
2761 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
2821 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
2881 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
2941 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
3001 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
3061 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
3121 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
3181 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
3241 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
3301 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
3361 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
3421 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
3481 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
3541 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
3601 tcgataagct tgatatcgaa ttcggagcac tgtcctccga acgtcggagc actgtcctcc
3661 gaacgtcgga gcactgtcct ccgaacgtcg gagcactgtc ctccgaacgg agcatgtcct
3721 ccgaacgtcg gagcactgtc ctccgaacga ctagttaggc gtgtacggtg ggaggcctat
3781 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
3841 gacctccata gaagacaccg ggaccgatcc agcctctcga cattcgttgg atcatgagcg
3901 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
3961 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
4021 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
4081 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
4141 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
4201 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
4261 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
4321 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
4381 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
4441 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
4501 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
4561 acctccctag caaactgggg cacaagctta attaagatcc ttgacttgcg gccgcaactc
4621 ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct gcatctaggc
4681 gccggaatta gatctctcga ggttaacgaa ttctaccggg taggggaggc gcttttccca
4741 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
4801 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
4861 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
4921 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
4981 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
5041 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
5101 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
5161 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
5221 tgcagcccaa gcttaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
5281 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
5341 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
5401 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
5461 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
5521 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
5581 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
5641 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
5701 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
5761 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
5821 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
5881 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
5941 acaagtaaag cggccgcgac tctagagtcg acctgcaggc atgcaagctt gcaggcatgc
6001 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
6061 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
6121 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
6181 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
6241 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
6301 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
6361 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
6421 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
6481 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
6541 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
6601 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
6661 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
6721 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
6781 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
6841 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
6901 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
6961 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
7021 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
7081 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
7141 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
7201 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
7261 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
7321 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
7381 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
7441 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
7501 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
7561 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
7621 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
7681 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
7741 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
7801 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
7861 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
7921 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
7981 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
8041 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
8101 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
8161 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
8221 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
8281 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
8341 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
8401 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
8461 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
8521 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
8581 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
8641 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
8701 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
8761 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
8821 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
8881 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
8941 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
9001 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
9061 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
9121 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
9181 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
9241 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
9301 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
9361 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
9421 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
9481 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
9541 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
9601 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
9661 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
9721 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
9781 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
9841 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
9901 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
9961 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
10021 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
10081 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
10141 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
10201 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
10261 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
10321 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
10381 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
10441 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
10501 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
10561 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
10621 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
10681 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
10741 taatcttttc aacgcctggc actgc
//
