| 质粒名称: | pRGE31 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 50929 |
| 存储实验室: | Yinong Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pUGW11 |
| 载体出品公司: | Nakagawa,T |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6034 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 1移除Bsa I位点。2插入含有水稻U3启动子和grnascaffold3的片段。通过LR反应插入Cas9。. |
| 载体类型: | 植物表达，CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | 水稻snornau3和双35S启动子 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13R(-48) |
| 3' 测序引物 | M13F(-20) |
|
文献引用方法 |
|
| 材料和方法部分: | pRGE31 was a gift from Yinong Yang (Addgene plasmid # 50929 ; http://n2t.net/addgene:50929 ; RRID:Addgene_50929) |
| 参考文献部分: | RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Xie K, Yang Y. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. 10.1093/mp/sst119 PubMed 23956122 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRGE31 9188 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by
rice snoRNA U3 promoter.
ACCESSION .
VERSION .
KEYWORDS pRGE31
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9188)
AUTHORS Xie K, Yang Y
TITLE RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
JOURNAL Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013
Aug 17.
PUBMED 23956122
REFERENCE 2 (bases 1 to 9188)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9188
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 26..49
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
terminator 71..323
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(332..349)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(332..348)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(341..363)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 561..1016
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(648..667)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 858..879
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(1033..1052)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1152..1174
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1212..1230)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1298..1402
/gene="bla"
/label=AmpR promoter
CDS 1403..2263
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGTRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1621..1640)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2434..3022
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2923..2942
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3176..3193
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3310..3331
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3346..3376
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3384..3400
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3389..3411
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 3408..3424
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3408..3424
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 3443..3823
/label=OsU3 promoter
/note="Oryza sativa (rice) snRNA U3 promoter"
misc_RNA 3840..3915
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 4481..4826
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 4769..4789
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
protein_bind 4848..4872
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 4881..4946
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 4953..4973
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4998..9098
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 9099..9146
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
protein_bind complement(9179..15)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
ORIGIN
1 cttgtacaaa gtggttgata acagcgacta caaggatgac gatgacaagg cttagagctc
61 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
121 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
181 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
241 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
301 ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa cgtcgtgact
361 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
481 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
541 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
601 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
661 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
721 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
781 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
841 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
901 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
961 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
1021 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
1081 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
1141 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
1201 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
1261 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
1321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
1381 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
1441 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
1501 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
1561 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
1621 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
1681 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
1741 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
1801 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
1861 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
1921 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
1981 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
2041 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
2101 tggagccggt gagcgtggca ctcgcggtat cattgcagca ctggggccag atggtaagcc
2161 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
2221 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
2281 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
2341 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
2401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
2461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
2521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
2581 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
2641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
2701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
2761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
2821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
2941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
3001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
3061 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
3121 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
3181 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
3241 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
3301 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
3361 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
3421 tgaccatgat tacgccaagc ttaaggaatc tttaaacata cgaacagatc acttaaagtt
3481 cttctgaagc aacttaaagt tatcaggcat gcatggatct tggaggaatc agatgtgcag
3541 tcagggacca tagcacaaga caggcgtctt ctactggtgc taccagcaaa tgctggaagc
3601 cgggaacact gggtacgttg gaaaccacgt gatgtgaaga agtaagataa actgtaggag
3661 aaaagcattt cgtagtgggc catgaagcct ttcaggacat gtattgcagt atgggccggc
3721 ccattacgca attggacgac aacaaagact agtattagta ccacctcggc tatccacata
3781 gatcaaagct gatttaaaag agttgtgcag atgatccgtg gcaggagacc gaggtctcgg
3841 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
3901 gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt
3961 ccgtttttag cgcgtgcatg cctgcaggtc cccagattag ccttttcaat ttcagaaaga
4021 atgctaaccc acagatggtt agagaggctt acgcagcagc actcatcaag acgatctacc
4081 cgagcaataa tctccaggaa atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa
4141 gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta
4201 ctattccagt atggacgatt caaggcttgc ttcacaaacc aaggcaagta atagagattg
4261 gagtctctaa aaaggtagtt cccactgaat caaaggccat ggagtcaaag attcaaatag
4321 aggacctaac agaactcgcc gtaaagactg gcgaacagtt catacagagt ctcttacgac
4381 tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacacactt gtctactcca
4441 aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg
4501 taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga
4561 tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg
4621 ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
4681 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
4741 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
4801 gaagttcatt tcatttggag agaacacggg ggactctaga gttatcaaca agtttgtaca
4861 aaaaagcagg ctccaccatg gactataagg accacgacgg agactacaag gatcatgata
4921 ttgattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc
4981 acggagtccc agcagccgac aagaagtaca gcatcggcct ggacatcggc accaactctg
5041 tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg
5101 gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg
5161 gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga
5221 agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca
5281 gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc
5341 accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct
5401 accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc
5461 tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc
5521 ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt
5581 tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac
5641 tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg
5701 gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact
5761 tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg
5821 acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc
5881 tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc
5941 ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga
6001 aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca
6061 agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca
6121 tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag
6181 aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc
6241 tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca
6301 accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg
6361 ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct
6421 ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga
6481 ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg
6541 agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa
6601 agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca
6661 accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg
6721 actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg
6781 atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc
6841 tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc
6901 tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat
6961 acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg
7021 gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc
7081 tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc
7141 agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg
7201 gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc
7261 ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga
7321 acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc
7381 tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc
7441 tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact
7501 acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg
7561 tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg
7621 tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa
7681 agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct
7741 tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg
7801 actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga
7861 tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc
7921 gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg
7981 ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt
8041 acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt
8101 acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg
8161 agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata
8221 agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga
8281 aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca
8341 gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca
8401 gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga
8461 aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga
8521 agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca
8581 tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct
8641 ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc
8701 tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac
8761 agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt
8821 tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca
8881 agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga
8941 ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt
9001 acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt
9061 acgagacacg gatcgacctg tctcagctgg gaggcgacaa aaggccggcg gccacgaaaa
9121 aggccggcca ggcaaaaaag aaaaagtaag aattcgcggc cgcactcgag atatctagac
9181 ccagcttt
//
