pBMTB-5

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品人: Lynch,M.D. and Gill,R.T.
载体名称: pBMTB-5
质粒类型: 广宿主载体;克隆载体;原核表达载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子: araBAD
载体大小: 4406 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 甲氧氨苄嘧啶
克隆菌株: DH5α 等
宿主细胞(系): 多个菌属
备注: --
产品目录号: --
组成型/诱导型: 诱导型

载体质粒图谱和多克隆位点信息

pBMTB-5载体图谱



载体简介

载体序列

LOCUS       DQ058728                4406 bp    DNA     circular SYN 08-MAY-2006
DEFINITION  Broad host range vector pBMTB-5, complete sequence.
ACCESSION   DQ058728
  AUTHORS   Lynch,M.D. and Gill,R.T.
  TITLE     Broad host range vectors for stable genomic library construction
  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
            80309, USA
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Broad host range vector pBMTB-5"
                     /mol_type="other DNA"
                     /db_xref="taxon:334960"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR terminator"
     gene            complement(104..1123)
                     /gene="mob"
                     /note="mob gene from pBBR1MCS, GenBank Accession Number
                     U02374"
     CDS             complement(104..1123)
                     /gene="mob"
                     /note="required for mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="Mob"
                     /protein_id="AAY99713.1"
                     /db_xref="GI:69048296"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
     gene            2117..2779
                     /gene="rep"
                     /note="rep gene from pBBR1, GenBank Accession Number
                     X66730"
     CDS             2117..2779
                     /gene="rep"
                     /note="required for replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAY99714.1"
                     /db_xref="GI:69048297"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            2876..3112
                     /gene="dhfrIIb"
                     /note="dhfrIIb gene from R388"
     CDS             2876..3112
                     /gene="dhfrIIb"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="AAY99715.1"
                     /db_xref="GI:69048298"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVG
                     WYCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     regulatory      3129..3160
                     /regulatory_class="terminator"
                     /note="TonB terminator"
     gene            complement(3188..4117)
                     /gene="araC"
     CDS             complement(3188..4117)
                     /gene="araC"
                     /note="regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC"
                     /protein_id="AAY99716.1"
                     /db_xref="GI:69048299"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
     regulatory      4125..4392
                     /regulatory_class="promoter"
                     /note="pBAD promoter"
ORIGIN      
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatggg
     2881 tcaaagtagc gatgaagcca acgctcccgt tgcagggcag tttgcgcttc ccctgagtgc
     2941 cacctttggc ttaggggatc gcgtacgcaa gaaatctggt gccgcttggc agggtcaagt
     3001 cgtcggttgg tattgcacaa aactcactcc tgaaggctat gcggtcgagt ccgaatccca
     3061 cccaggctca gtgcaaattt atcctgtggc tgcacttgaa cgtgtggcct aagaatggat
     3121 ccccctcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg gaggtcaggt
     3181 atgattttta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga
     3241 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt
     3301 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
     3361 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
     3421 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
     3481 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat
     3541 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
     3601 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
     3661 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat
     3721 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
     3781 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg
     3841 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca
     3901 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
     3961 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat
     4021 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc
     4081 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct
     4141 tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac
     4201 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa
     4261 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca
     4321 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
     4381 cgtttttttg ggctagagaa ttcgtc
//

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