| 出品人: | Lynch,M.D. and Gill,R.T. |
|---|---|
| 载体名称: | pBMTB-5 |
| 质粒类型: | 广宿主载体；克隆载体；原核表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | araBAD |
| 载体大小: | 4406 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 甲氧氨苄嘧啶 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pBMTB-5载体质粒基本信息
pBMTB-5质粒图谱载体图谱和pBMTB-5载体序列质粒序列多克隆位点信息
pBMTB-5质粒载体简介
pBMTB-5质粒序列载体序列
LOCUS DQ058728 4406 bp DNA circular SYN 08-MAY-2006
DEFINITION Broad host range vector pBMTB-5, complete sequence.
ACCESSION DQ058728
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Broad host range vectors for stable genomic library construction
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
FEATURES Location/Qualifiers
source 1..4406
/organism="Broad host range vector pBMTB-5"
/mol_type="other DNA"
/db_xref="taxon:334960"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene complement(104..1123)
/gene="mob"
/note="mob gene from pBBR1MCS, GenBank Accession Number
U02374"
CDS complement(104..1123)
/gene="mob"
/note="required for mobilization"
/codon_start=1
/transl_table=11
/product="Mob"
/protein_id="AAY99713.1"
/db_xref="GI:69048296"
/translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
KPLLS"
gene 2117..2779
/gene="rep"
/note="rep gene from pBBR1, GenBank Accession Number
X66730"
CDS 2117..2779
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99714.1"
/db_xref="GI:69048297"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 2876..3112
/gene="dhfrIIb"
/note="dhfrIIb gene from R388"
CDS 2876..3112
/gene="dhfrIIb"
/codon_start=1
/transl_table=11
/product="dihydrofolate reductase"
/protein_id="AAY99715.1"
/db_xref="GI:69048298"
/translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVG
WYCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
regulatory 3129..3160
/regulatory_class="terminator"
/note="TonB terminator"
gene complement(3188..4117)
/gene="araC"
CDS complement(3188..4117)
/gene="araC"
/note="regulator"
/codon_start=1
/transl_table=11
/product="AraC"
/protein_id="AAY99716.1"
/db_xref="GI:69048299"
/translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
regulatory 4125..4392
/regulatory_class="promoter"
/note="pBAD promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatggg
2881 tcaaagtagc gatgaagcca acgctcccgt tgcagggcag tttgcgcttc ccctgagtgc
2941 cacctttggc ttaggggatc gcgtacgcaa gaaatctggt gccgcttggc agggtcaagt
3001 cgtcggttgg tattgcacaa aactcactcc tgaaggctat gcggtcgagt ccgaatccca
3061 cccaggctca gtgcaaattt atcctgtggc tgcacttgaa cgtgtggcct aagaatggat
3121 ccccctcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg gaggtcaggt
3181 atgattttta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga
3241 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt
3301 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
3361 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
3421 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
3481 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat
3541 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
3601 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
3661 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat
3721 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
3781 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg
3841 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca
3901 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
3961 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat
4021 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc
4081 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct
4141 tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac
4201 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa
4261 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca
4321 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
4381 cgtttttttg ggctagagaa ttcgtc
//
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