| 质粒名称: | pAAV-fPV-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22914 |
| 存储实验室: | Edward Callaway |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4000 bp |
| 完整质粒大小: | 8100 bp |
| 载体类型: | AAV,哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | fugu parvalbumin promoter driving GFP |
| 物种来源: | takifugu rubripes (河豚) |
| 插入大小: | 4100 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | Unknown |
| 3' 测序引物 | SP6 |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-fPV-GFP was a gift from Edward Callaway (爱迪基因 plasmid # 22914) |
| 参考文献部分: | Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM. Front Neural Circuits. 2009 . 3():19. 10.3389/neuro.04.019.2009 PubMed 19949461 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV-fPV-GFP 8113 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-fPV-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8113)
AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
Callaway EM
TITLE Short Promoters in Viral Vectors Drive Selective Expression in
Mammalian Inhibitory Neurons, but do not Restrict Activity to
Specific Inhibitory Cell-Types.
JOURNAL Front Neural Circuits. 2009 . 3():19.
PUBMED 19949461
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-fPV-GFP|
FEATURES Location/Qualifiers
source 1..8113
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 48..177
/label=AAV2 ITR
intron 2752..3227
/label=-beta-globin intron
/note="internally truncated intron from human beta-globin"
primer_bind 3309..3325
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3309..3325
/label=pBluescriptSK
/note="For pBluescript vector"
regulatory 3358..3367
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3364..4080
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3409..3430)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3670..3689)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4017..4038
/label=EGFP-C
/note="EGFP, forward primer"
promoter complement(4141..4159)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4142..4159)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4179..4196)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4185..4296
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4440..4569
/label=AAV2 ITR
rep_origin 4940..5453
/direction=RIGHT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5027..5046)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5237..5258
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5935..5954)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6054..6076
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6114..6132)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6200..6304
/gene="bla"
/label=AmpR promoter
CDS 6305..7165
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6523..6542)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7336..7924
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7825..7844
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8078..8095
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg cgcgctcgct
61 cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct
121 cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt
181 agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctaga ggatccttcg
241 aaaagcttgt atacggccgg ccacgcgtac aagatggata cgactgccag cttacccctg
301 cctggatctt cgttaattac catcagaaag actaaattct gctttaattc agtttctgat
361 acctattttt gagctgacac gagcttgtga aaacaactgc tggttgattg cgtgtttgca
421 ttgtcacatc tgtacagaat gtcagtggtt gcacaatttc tgggggggat tctccaaact
481 acagcgctgt tttaccggac tgagcaatgt caactacata ttaaaaagtt aaagggcaga
541 ttttctatct aacgtgcaga ttatgttgga tttaatcaag aacttcatag gactgttctc
601 tccttctgat tctcccataa ttcctacagt ctatgtgaca gcgaacacat catgctgccc
661 tcttttctca gccttgagcc tgtgtgtgtg tgtgtgtgtg tgcgtgtgtg tgtgtgtgtg
721 tgtgtgtgtt agtgatgggc tctggagctt ctctgaacag atatttcaga gcttcttacg
781 taacaggtcc cgctgcacct caatccttaa ctacagatgt gtcccagctg atccaaaacg
841 ggccatatga cttgatcagg tgttaacagt ggtggaaaaa accctgggag aggacacaag
901 cagaaaagag cagtgcatgg gtgaaagtaa aaaaaacttc atgcctgttt ttagaggtaa
961 taatggagca tgtattgatt tatgtggagt gtgtaggtgt gagcatcatt atttaatcaa
1021 ccttccctgt gggtgctgcc ctgtgattgc tgatgtaatg aggtaccaaa gcatcaatat
1081 ctggaggtag ctctgcaaac atgcgatgga agcatgaaaa tctgcctcgg tttacagtct
1141 cgggccgcct ctcagcactg ggatggatca cactatttga ttgaaggcac atgtgccaca
1201 gctctgcaaa ggtggagcaa cctgcacatt agctcttaag tcacaaatca ctctgcaaaa
1261 gtctaaaatc accgagaatg ataaaacttt taaaatgcag agatcctgaa aaaacacaag
1321 gcataaaaaa gaaagacaaa tatgttgtct ccatagtaac catatctcta ataaagaccc
1381 gaccatgcag atagttttga ccttgatata ttttttaggt tgattacaac ctctgaaatc
1441 ctaaatgttg gtgctaaaaa gatttaattt taactatctg taccagggga aggtggacgg
1501 tttggcctct cattacttaa cggtttggcc gtctgacaac caaacgccca acaacttggt
1561 ggattccttt taaaaccgca ccatctccca cgcacgcccc cacgcagcag ccgtaacatt
1621 agcactaaat ataccactct aaccagtgcc tgcagtgcag tcgtgcatgt ttggcaaaga
1681 gtggggcttc cttctcccac tctgctcagc caactgcctg accaggacct acggggtcgc
1741 gggagggttt gtgccgtgca gttcttgcca tctcggttct ggtcagtttt gtcaactata
1801 ttgtaatcac cgtgattgac aggtgaactc tggttgcaca gaaggaggtt gaactgcaca
1861 gaaggagaac ttcagctcag actgctcacg ctggcatgcc tcgatctaaa ataaactctc
1921 tccaactttt ctttccttga cattgtaggt taaatcagaa gtctgagctc tgctctgaac
1981 tttccaactc tttctttctg tcattattga gaagcgtctc tataaaacca attagactta
2041 aacatacata catacataca tacatacata catacataca tacatacata catacataca
2101 tacatacata tggcatgtga tcaaacatat gtgatatctt tgtctgagat gcctgagcct
2161 aggaacaggt tagagggcca gaacatcgtg acatacctga actgtctgtg gtaacttaat
2221 caaagataac tgagctgcaa tcactgggca gtttcagcat cttctcacag cccagagcac
2281 ttcagcaaac acttgttaaa gaagaaatta gactctccaa agacttctgt gtcataatca
2341 tgaagacaag agatattttg gcttcgattt gtacatcatt acctgtactt tgaagggata
2401 aaaataaaac aaaagcttca acatctggtc agcacacatg gttcggtctc tgtgtacagt
2461 cgcagacggc tcaggcctga cccctatcga cacctgtctg ctactacaac agatctatta
2521 cactcttcgc agttaaaaat gatttacttc atttaaaaaa agtccgccat ccaacaaaac
2581 gtgtgttctg gcatgcagat caaacatcag attagggatt tttgtacact accttcctca
2641 gttacagctg atatatatct tttctgcttt tccactgacc tttaacctcc tcctgtcttt
2701 tcagctggca aagctagcgg cgcgccgagc tcggatcctg agaacttcag ggtgagtcta
2761 tgggaccctt gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag
2821 gggagaagta acagggtaca catattgacc aaatcagggt aattttgcat ttgtaatttt
2881 aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct
2941 aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa
3001 agaataacag tgataatttc tgggttaagg caatagcaat atttctgcat ataaatattt
3061 ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca
3121 gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct
3181 aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagctc ctgggcaacg
3241 tgctggtctg tgtgctggcc catcactttg gcaaagcacg tgaattcgaa gatctggccg
3301 cctcggcctc tagaactagt ggatcccccg ggctgcagga attcgatgct accggtcgcc
3361 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
3421 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
3481 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
3541 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
3601 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
3661 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
3721 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
3781 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
3841 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
3901 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
3961 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
4021 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
4081 tccggactca gatcctacta gtaggatctc gagtttaaac cgtacgggaa acggccctat
4141 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
4201 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
4261 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
4321 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcgc
4381 ggccgctcta gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa
4441 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
4501 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
4561 agcgcgcagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
4621 cagcctgaat ggcgaatggc gattccgttg caatggctgg cggtaatatt gttctggata
4681 ttaccagcaa ggccgatagt ttgagttctt ctactcaggc aagtgatgtt attactaatc
4741 aaagaagtat tgcgacaacg gttaatttgc gtgatggaca gactctttta ctcggtggcc
4801 tcactgatta taaaaacact tctcaggatt ctggcgtacc gttcctgtct aaaatccctt
4861 taatcggcct cctgtttagc tcccgctctg attctaacga ggaaagcacg ttatacgtgc
4921 tcgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
4981 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
5041 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
5101 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
5161 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
5221 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
5281 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
5341 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaaata
5401 tttgcttata caatcttcct gtttttgggg cttttctgat tatcaaccgg ggtacatatg
5461 attgacatgc tagttttacg attaccgttc atcgattctc ttgtttgctc cagactctca
5521 ggcaatgacc tgatagcctt tgtagagacc tctcaaaaat agctaccctc tccggcatga
5581 atttatcagc tagaacggtt gaatatcata ttgatggtga tttgactgtc tccggccttt
5641 ctcacccgtt tgaatcttta cctacacatt actcaggcat tgcatttaaa atatatgagg
5701 gttctaaaaa tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg
5761 gtcataatgt ttttggtaca accgatttag ctttatgctc tgaggcttta ttgcttaatt
5821 ttgctaattc tttgccttgc ctgtatgatt tattggatgt tggaatcgcc tgatgcggta
5881 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
5941 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
6001 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
6061 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
6121 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
6181 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
6241 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
6301 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
6361 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
6421 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
6481 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
6541 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
6601 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
6661 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
6721 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
6781 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
6841 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
6901 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
6961 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
7021 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
7081 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
7141 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
7201 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
7261 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
7321 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
7381 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
7441 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
7501 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
7561 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
7621 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
7681 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
7741 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
7801 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
7861 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
7921 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
7981 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
8041 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
8101 ggaagagcgc cca
//
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