pAAVLINK-ATM-B

LOCUS       pAAVLINK-ATM-B               6897 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     protein_bind    complement(145..178)
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          179..270
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             271..4053
                     /codon_start=1
                     /gene="ATM"
                     /gene_synonym="AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1"
                     /product="serine-protein kinase ATM isoform a"
                     /EC_number="2.7.11.1"
                     /label=ATM
                     /note="isoform a is encoded by transcript variant 2; TEL1, 
                     telomere maintenance 1, homolog; AT mutated; serine-protein
                     kinase ATM; A-T mutated; ataxia telangiectasia mutated; 
                     serine/threonine kinase ATM"
                     /db_xref="GI:71902540"
                     /db_xref="CCDS:CCDS31669.1"
                     /db_xref="GeneID:472"
                     /db_xref="HGNC:HGNC:795"
                     /db_xref="MIM:607585"
                     /protein_id="NP_000042.3"
                     /translation="DAQGQFLTVIGAFWHLTKERKYIFSVRMALVNCLKTLLEADPYSK
                     WAILNVMGKDFPVNEVFTQFLADNHHQVRMLAAESINRLFQDTKGDSSRLLKALPLKLQ
                     QTAFENAYLKAQEGMREMSHSAENPETLDEIYNRKSVLLTLIAVVLSCSPICEKQALFA
                     LCKSVKENGLEPHLVKKVLEKVSETFGYRRLEDFMASHLDYLVLEWLNLQDTEYNLSSF
                     PFILLNYTNIEDFYRSCYKVLIPHLVIRSHFDEVKSIANQIQEDWKSLLTDCFPKILVN
                     ILPYFAYEGTRDSGMAQQRETATKVYDMLKSENLLGKQIDHLFISNLPEIVVELLMTLH
                     EPANSSASQSTDLCDFSGDLDPAPNPPHFPSHVIKATFAYISNCHKTKLKSILEILSKS
                     PDSYQKILLAICEQAAETNNVYKKHRILKIYHLFVSLLLKDIKSGLGGAWAFVLRDVIY
                     TLIHYINQRPSCIMDVSLRSFSLCCDLLSQVCQTAVTYCKDALENHLHVIVGTLIPLVY
                     EQVEVQKQVLDLLKYLVIDNKDNENLYITIKLLDPFPDHVVFKDLRITQQKIKYSRGPF
                     SLLEEINHFLSVSVYDALPLTRLEGLKDLRRQLELHKDQMVDIMRASQDNPQDGIMVKL
                     VVNLLQLSKMAINHTGEKEVLEAVGSCLGEVGPIDFSTIAIQHSKDASYTKALKLFEDK
                     ELQWTFIMLTYLNNTLVEDCVKVRSAAVTCLKNILATKTGHSFWEIYKMTTDPMLAYLQ
                     PFRTSRKKFLEVPRFDKENPFEGLDDINLWIPLSENHDIWIKTLTCAFLDSGGTKCEIL
                     QLLKPMCEVKTDFCQTVLPYLIHDILLQDTNESWRNLLSTHVQGFFTSCLRHFSQTSRS
                     TTPANLDSESEHFFRCCLDKKSQRTMLAVVDYMRRQKRPSSGTIFNDAFWLDLNYLEVA
                     KVAQSCAAHFTALLYAEIYADKKSMDDQEKRSLAFEEGSQSTTISSLSEKSKEETGISL
                     QDLLLEIYRSIGEPDSLYGCGGGKMLQPITRLRTYEHEAMWGKALVTYDLETAIPSSTR
                     QAGIIQALQNLGLCHILSVYLKGLDYENKDWCPELEELHYQAAWRNMQWDHCTSVSKEV
                     EGTSYHESLYNALQSLRDREFSTFYESLKYARVKEVEEMCKRSLESVYSLYPTLSRLQA
                     IGELESIGELFSRSVTHRQLSEVYIKWQKHSQLLKDSDFSFQEPIMALRTVILEILMEK
                     EMDNSQRECIKDILTKHLVELSILARTFKNTQLPE"
     misc_feature    1606..1611
                     /label=SDS
     intron          4054..4094
                     /label=Splicing Donor
     misc_feature    4098..4131
                     /label=lox2272-JT1571
     repeat_region   4160..4300
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4160..4289
                     /label=AAV2 ITR
     repeat_region   4161..4301
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4375..4830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5112..5216
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5217..6077
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6248..6836
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtataact tcgtatagca tacattatag caatttatga
      181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
      241 ctgacatcca ctttgccttt ctctccacag ggatgctcaa ggacagtttc ttacagtaat
      301 tggagcattt tggcatctaa caaaggagag gaaatatata ttctctgtaa gaatggccct
      361 agtaaattgc cttaaaactt tgcttgaggc tgatccttat tcaaaatggg ccattcttaa
      421 tgtaatggga aaagactttc ctgtaaatga agtatttaca caatttcttg ctgacaatca
      481 tcaccaagtt cgcatgttgg ctgcagagtc aatcaataga ttgttccagg acacgaaggg
      541 agattcttcc aggttactga aagcacttcc tttgaagctt cagcaaacag cttttgaaaa
      601 tgcatacttg aaagctcagg aaggaatgag agaaatgtcc catagtgctg agaaccctga
      661 aactttggat gaaatttata atagaaaatc tgttttactg acgttgatag ctgtggtttt
      721 atcctgtagc cctatctgcg aaaaacaggc tttgtttgcc ctgtgtaaat ctgtgaaaga
      781 gaatggatta gaacctcacc ttgtgaaaaa ggttttagag aaagtttctg aaacttttgg
      841 atatagacgt ttagaagact ttatggcatc tcatttagat tatctggttt tggaatggct
      901 aaatcttcaa gatactgaat acaacttatc ttcttttcct tttattttat taaactacac
      961 aaatattgag gatttctata gatcttgtta taaggttttg attccacatc tggtgattag
     1021 aagtcatttt gatgaggtga agtccattgc taatcagatt caagaggact ggaaaagtct
     1081 tctaacagac tgctttccaa agattcttgt aaatattctt ccttattttg cctatgaggg
     1141 taccagagac agtgggatgg cacagcaaag agagactgct accaaggtct atgatatgct
     1201 taaaagtgaa aacttattgg gaaaacagat tgatcactta ttcattagta atttaccaga
     1261 gattgtggtg gagttattga tgacgttaca tgagccagca aattctagtg ccagtcagag
     1321 cactgacctc tgtgactttt caggggattt ggatcctgct cctaatccac ctcattttcc
     1381 atcgcatgtg attaaagcaa catttgccta tatcagcaat tgtcataaaa ccaagttaaa
     1441 aagcatttta gaaattcttt ccaaaagccc tgattcctat cagaaaattc ttcttgccat
     1501 atgtgagcaa gcagctgaaa caaataatgt ttataagaag cacagaattc ttaaaatata
     1561 tcacctgttt gttagtttat tactgaaaga tataaaaagt ggcttaggag gagcttgggc
     1621 ctttgttctt cgagacgtta tttatacttt gattcactat atcaaccaaa ggccttcttg
     1681 tatcatggat gtgtcattac gtagcttctc cctttgttgt gacttattaa gtcaggtttg
     1741 ccagacagcc gtgacttact gtaaggatgc tctagaaaac catcttcatg ttattgttgg
     1801 tacacttata ccccttgtgt atgagcaggt ggaggttcag aaacaggtat tggacttgtt
     1861 gaaatactta gtgatagata acaaggataa tgaaaacctc tatatcacga ttaagctttt
     1921 agatcctttt cctgaccatg ttgtttttaa ggatttgcgt attactcagc aaaaaatcaa
     1981 atacagtaga ggaccctttt cactcttgga ggaaattaac cattttctct cagtaagtgt
     2041 ttatgatgca cttccattga caagacttga aggactaaag gatcttcgaa gacaactgga
     2101 actacataaa gatcagatgg tggacattat gagagcttct caggataatc cgcaagatgg
     2161 gattatggtg aaactagttg tcaatttgtt gcagttatcc aagatggcaa taaaccacac
     2221 tggtgaaaaa gaagttctag aggctgttgg aagctgcttg ggagaagtgg gtcctataga
     2281 tttctctacc atagctatac aacatagtaa agatgcatct tataccaagg cccttaagtt
     2341 atttgaagat aaagaacttc agtggacctt cataatgctg acctacctga ataacacact
     2401 ggtagaagat tgtgtcaaag ttcgatcagc agctgttacc tgtttgaaaa acattttagc
     2461 cacaaagact ggacatagtt tctgggagat ttataagatg acaacagatc caatgctggc
     2521 ctatctacag ccttttagaa catcaagaaa aaagttttta gaagtaccca gatttgacaa
     2581 agaaaaccct tttgaaggcc tggatgatat aaatctgtgg attcctctaa gtgaaaatca
     2641 tgacatttgg ataaagacac tgacttgtgc ttttttggac agtggaggca caaaatgtga
     2701 aattcttcaa ttattaaagc caatgtgtga agtgaaaact gacttttgtc agactgtact
     2761 tccatacttg attcatgata ttttactcca agatacaaat gaatcatgga gaaatctgct
     2821 ttctacacat gttcagggat ttttcaccag ctgtcttcga cacttctcgc aaacgagccg
     2881 atccacaacc cctgcaaact tggattcaga gtcagagcac tttttccgat gctgtttgga
     2941 taaaaaatca caaagaacaa tgcttgctgt tgtggactac atgagaagac aaaagagacc
     3001 ttcttcagga acaattttta atgatgcttt ctggctggat ttaaattatc tagaagttgc
     3061 caaggtagct cagtcttgtg ctgctcactt tacagcttta ctctatgcag aaatctatgc
     3121 agataagaaa agtatggatg atcaagagaa aagaagtctt gcatttgaag aaggaagcca
     3181 gagtacaact atttctagct tgagtgaaaa aagtaaagaa gaaactggaa taagtttaca
     3241 ggatcttctc ttagaaatct acagaagtat aggggagcca gatagtttgt atggctgtgg
     3301 tggagggaag atgttacaac ccattactag actacgaaca tatgaacacg aagcaatgtg
     3361 gggcaaagcc ctagtaacat atgacctcga aacagcaatc ccctcatcaa cacgccaggc
     3421 aggaatcatt caggccttgc agaatttggg actctgccat attctttccg tctatttaaa
     3481 aggattggat tatgaaaata aagactggtg tcctgaacta gaagaacttc attaccaagc
     3541 agcatggagg aatatgcagt gggaccattg cacttccgtc agcaaagaag tagaaggaac
     3601 cagttaccat gaatcattgt acaatgctct acaatctcta agagacagag aattctctac
     3661 attttatgaa agtctcaaat atgccagagt aaaagaagtg gaagagatgt gtaagcgcag
     3721 ccttgagtct gtgtattcgc tctatcccac acttagcagg ttgcaggcca ttggagagct
     3781 ggaaagcatt ggggagcttt tctcaagatc agtcacacat agacaactct ctgaagtata
     3841 tattaagtgg cagaaacact cccagcttct caaggacagt gattttagtt ttcaggagcc
     3901 tatcatggct ctacgcacag tcattttgga gatcctgatg gaaaaggaaa tggacaactc
     3961 acaaagagaa tgtattaagg acattctcac caaacacctt gtagaactct ctatactggc
     4021 cagaactttc aagaacactc agctccctga aaggtaagta tcaaggttac aagacaggtt
     4081 taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta tgaattccac
     4141 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4201 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     4261 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     4321 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     4381 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4441 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4501 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4561 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     4621 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4681 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     4741 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4801 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     4861 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4921 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4981 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5041 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5101 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5161 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5221 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5281 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5341 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5401 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5461 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5521 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5581 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5641 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5701 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5761 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5821 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5881 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5941 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6001 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6061 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6121 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6181 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6241 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6301 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6361 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6421 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6481 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6541 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6601 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6661 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6721 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6781 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6841 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//