| 质粒名称: | mApple-Peroxisomes-2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 54627 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mApple |
| 空载体大小: | 4750 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mApple-Peroxisomes |
| 标签/融合蛋白: | 过氧化物酶体靶向信号1（PTS1；Ser-Lys-Leu）（插入物上的C末端） |
|
文献引用方法 |
|
| 材料和方法部分: |
mApple-Peroxisomes-2 was a gift from Michael Davidson (Addgene plasmid # 54627 ; http://n2t.net/addgene:54627 ; RRID:Addgene_54627)
|
买家导航
mApple-Peroxisomes-2载体质粒基本信息
mApple-Peroxisomes-2质粒图谱载体图谱和mApple-Peroxisomes-2载体序列质粒序列多克隆位点信息
mApple-Peroxisomes-2质粒载体简介
mApple-Peroxisomes-2质粒序列载体序列
以下序列为质粒部分序列
LOCUS mApple-Peroxisomes-2 833 bp DNA linear UNA 15-DEC-2025
DEFINITION Localization: Peroxisomes, Excitation: 568, Emission: 592.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE natural DNA sequence
ORGANISM unspecified
REFERENCE 1 (bases 1 to 833)
AUTHORS Davidson Lab
TITLE Michael Davidson Empty Backbones
JOURNAL unpublished
REFERENCE 2 (bases 1 to 833)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"unpublished"
COMMENT Sequence Label: mApple-Peroxisomes-2
FEATURES Location/Qualifiers
source 1..833
/mol_type="genomic DNA"
/organism="unspecified"
misc_feature 17..97
/label=MCS
/note="multiple cloning site"
regulatory 99..108
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 105..812
/codon_start=1
/product="photostable monomeric derivative of DsRed (Shaner
et al., 2008)"
/label=mApple
/note="mammalian codon-optimized"
/translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind 518..537
/label=mCherry-F
/note="mCherry, forward primer"
ORIGIN
1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
121 aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
181 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct
241 ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
301 tgtcccctca gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg
361 actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg
421 acggcggcat tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca
481 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
541 tgggctggga ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga
601 tcaagaagag gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct
661 acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca
721 tcgtgtccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
781 actccaccgg cggcatggac gagctgtaca agtccggatc caagctgtag cgg
//
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mApple-Peroxisomes-2质粒载体应用举例
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