| 质粒名称: | pLVPRT-tTR-KRAB |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 11648 |
| 存储实验室: | Patrick Aebischer, Didier Trono |
|
载体骨架基本信息 |
|
| 载体名称: | None |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 11583 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | hPrion, GFP, tTR-KRAB, Tet-on |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | See map |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | pLVPRT-tTR-KRAB was a gift from Patrick Aebischer & Didier Trono (Addgene plasmid # 11648) |
| 参考文献部分: | A versatile tool for conditional gene expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006 Feb . 3(2):109-16. 10.1038/nmeth846 PubMed 16432520 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVPRT-tTR-KRAB 11583 bp DNA SYN
DEFINITION pLVPRT-tTR-KRAB
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..11583
/organism="pLVPRT-tTR-KRAB"
/mol_type="other DNA"
misc_feature 461..641
/label="truncHIV-1_3_LTR"
misc_feature 461..641
/label="HIV-1_5_LTR"
misc_feature 752..796
/label="HIV-1_psi_pack"
CDS 1184..1684
/label="ORF frame 2"
misc_feature 1306..1539
/label="RRE"
misc_feature 2359..2634
/label="synth_int"
CDS 2723..3472
/label="ORF frame 2"
gene 2726..3439
/label="EGFP"
/gene="EGFP"
misc_feature complement(2768..2789)
/label="EGFP_N_primer"
misc_feature 2906..2935
/label="Y66 (EGFP)"
misc_feature 3376..3397
/label="EGFP_C_primer"
misc_feature 3523..4085
/label="IRES"
CDS 4089..5096
/label="ORF frame 3"
misc_feature 4104..4703
/label="TetR"
misc_feature 4710..4730
/label="SV40 NLS"
misc_feature 4716..4730
/label="NLS"
misc_feature 5171..5758
/label="WPRE"
CDS complement(5256..5759)
/label="ORF frame 2"
CDS 5259..5819
/label="ORF frame 3"
misc_feature 5829..5844
/label="cPPT"
misc_feature 5829..5850
/label="U3PPT"
misc_feature 5881..6168
/label="TRE"
misc_feature 6204..6384
/label="HIV-1_5_LTR"
misc_feature 6204..6384
/label="truncHIV-1_3_LTR"
misc_feature complement(6410..6427)
/label="Sp6_primer"
gene 6665..6928
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(6717..6736)
/label="pBRrevBam_primer"
misc_feature 7069..7091
/label="pGEX_3_primer"
promoter 7250..7278
/label="AmpR_promoter"
gene 7320..8180
/label="Ampicillin"
/gene="Ampicillin"
CDS 7320..8180
/label="ORF frame 3"
rep_origin 8335..8954
/label="pBR322_origin"
misc_feature complement(9180..9200)
/label="pBABE_3_primer"
misc_feature complement(9186..9639)
/label="SV40_enhancer"
promoter 9198..9466
/label="SV40_promoter"
rep_origin 9365..9442
/label="SV40_origin"
misc_feature 9427..9446
/label="SV40pro_F_primer"
misc_feature 10647..10662
/label="SV40_int"
misc_feature 10668..10715
/label="SV40_3_splice"
CDS 10792..11265
/label="ORF frame 1"
misc_feature 10843..10863
/label="SV40 NLS"
misc_feature 10849..10863
/label="NLS"
terminator 11291..11422
/label="SV40_PA_terminator"
misc_feature 11379..11398
/label="EBV_rev_primer"
ORIGIN
1 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
61 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
121 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
181 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
241 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
301 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
361 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
421 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
481 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
541 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
601 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
661 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
721 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
781 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
841 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
901 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
961 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
1021 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
1081 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
1141 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
1201 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
1261 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
1321 tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc tgacggtaca
1381 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
1441 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
1501 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
1561 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
1621 acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
1681 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
1741 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
1801 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
1861 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
1921 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
1981 gacagagaca gattcgagat ctttacagtc aaatgagcca cgtcagggag cgatggcacc
2041 cgcaggcggt atcaactgat gcaagtgttc aagcgaatct caactcgttt tttccggtga
2101 ctcattcccg gccctggttg gcagcgctgc accctttaac ttaaacctcg gccggccgcc
2161 cgccgggggc acagagtgtg cgccgggccg cgcggcaatt ggtccccgcg ccgacctccg
2221 cccgcgagcg ccgccgcttc ccttccccgc cccgcgtccc tccccctcgg ccccgcgcgt
2281 cgcctgtcct ccgagccagt cgctgacagc cgcggcgccg cgagcttctc ctctcctcac
2341 gaagatccta gaattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa
2401 aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat
2461 tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa
2521 tctttttgtt gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga
2581 caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcggac
2641 tttcgatatc aagcttatcg atacccgtcg acctcgagat ccaggcctaa gcttacgcgt
2701 cctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
2761 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
2821 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
2881 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
2941 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
3001 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
3061 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
3121 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
3181 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
3241 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
3301 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
3361 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
3421 catggacgag ctgtacaagt ccggactcag atctcgacta gctagtagct agctagctag
3481 tcgagctcaa gcttcgaatt cccgggctgc aggaattccg cccccccccc cctaacgtta
3541 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
3601 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
3661 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
3721 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
3781 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
3841 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
3901 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
3961 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
4021 taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
4081 ataataccat ggctagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
4141 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
4201 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
4261 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
4321 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
4381 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
4441 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
4501 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
4561 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
4621 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
4681 aaaaacaact taaatgtgaa agtgggtcgc caaaaaagaa gagaaaggtc gacggcggtg
4741 gtgctttgtc tcctcagcac tctgctgtca ctcaaggaag tatcatcaag aacaaggagg
4801 gcatggatgc taagtcacta actgcctggt cccggacact ggtgaccttc aaggatgtat
4861 ttgtggactt caccagggag gagtggaagc tgctggacac tgctcagcag atcgtgtaca
4921 gaaatgtgat gctggagaac tataagaacc tggtttcctt gggttatcag cttactaagc
4981 cagatgtgat cctccggttg gagaagggag aagagccctg gctggtggag agagaaattc
5041 accaagagac ccatcctgat tcagagactg catttgaaat caaatcatca gtttaaggat
5101 ctcgactagc tagtagctag ctagctagtc gagctcaact tcgaattcga tgggactagt
5161 catatgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
5221 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
5281 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
5341 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
5401 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
5461 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
5521 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
5581 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
5641 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
5701 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
5761 ggtacgtatg gccaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
5821 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
5881 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
5941 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
6001 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
6061 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
6121 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgga
6181 tcgatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
6241 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
6301 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
6361 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
6421 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
6481 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
6541 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
6601 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
6661 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
6721 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
6781 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
6841 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
6901 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
6961 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
7021 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
7081 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
7141 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
7201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
7261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
7321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
7381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
7441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
7501 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
7561 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
7621 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
7681 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
7741 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
7801 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
7861 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
7921 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
7981 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
8041 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
8101 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
8161 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
8221 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
8281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
8341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
8401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
8461 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
8521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
8581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
8641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
8701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
8761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
8821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
8881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
8941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
9001 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
9061 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
9121 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
9181 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
9241 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
9301 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
9361 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
9421 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
9481 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
9541 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
9601 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
9661 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
9721 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
9781 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
9841 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
9901 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
9961 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
10021 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
10081 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
10141 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
10201 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
10261 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
10321 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
10381 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
10441 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
10501 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
10561 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
10621 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
10681 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
10741 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
10801 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
10861 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
10921 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
10981 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
11041 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
11101 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
11161 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
11221 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
11281 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
11341 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
11401 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
11461 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct
11521 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
11581 act
//
