pAAVLINK-EF1a-HA-5'-ASXL3

LOCUS       pAAVLINK-EF1a-HA-5'-ASXL3               7005 bp DNA     circular SYN 19-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        146..688
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             737..4145
                     /codon_start=1
                     /label=ASXL3
                     /translation="KDKRKKKDRTWAEAARLALEKHPNSPMTAKQILEVIQKEGLKETS
                     GTSPLACLNAMLHTNTRIGDGTFFKIPGKSGLYALKKEESSCPADGTLDLVCESELDGT
                     DMAEANAHGEENGVCSKQVTDEASSTRDSSLTNTAVQSKLVSSFQQHTKKALKQALRQQ
                     QKRRNGVSMMVNKTVPRVVLTPLKVSDEQSDSPSGSESKNGEADSSDKEMKHGQKSPTG
                     KQTSQHLKRLKKSGLGHLKWTKAEDIDIETPGSILVNTNLRALINKHTFASLPQHFQQY
                     LLLLLPEVDRQMGSDGILRLSTSALNNEFFAYAAQGWKQRLAEGEFTPEMQLRIRQEIE
                     KEKKTEPWKEKFFERFYGEKLGMSREESVKLTTGPNNAGAQSSSSCGTSGLPVSAQTAL
                     AEQQPKSMKSPASPEPGFCATLCPMVEIPPKDIMAELESEDILIPEESVIQEEIAEEVE
                     TSICECQDENHKTIPEFSEEAESLTNSHEEPQIAPPEDNLESCVMMNDVLETLPHIEVK
                     IEGKSESPQEEMTVVIDQLEVCDSLIPSTSSMTHVSDTEHKESETAVETSTPKIKTGSS
                     SLEGQFPNEGIAIDMELQSDPEEQLSENACISETSFSSESPEGACTSLPSPGGETQSTS
                     EESCTPASLETTFCSEVSSTENTDKYNQRNSTDENFHASLMSEISPISTSPEISEASLM
                     SNLPLTSEASPVSNLPLTSETSPMSDLPLTSETSSVSSMLLTSETTFVSSLPLPSETSP
                     ISNSSINERMAHQQRKSPSVSEEPLSPQKDESSATAKPLGENLTSQQKNLSNTPEPIIM
                     SSSSIAPEAFPSEDLHNKTLSQQTCKSHVDTEKPYPASIPELASTEMIKVKNHSVLQRT
                     EKKVLPSPLELSVFSEGTDNKGNELPSAKLQDKQYISSVDKAPFSEGSRNKTHKQGSTQ
                     SRLETSHTSKSSEPSKSPDGIRNESRDSEISKRKTAEQHSFGICKEKRARIEDDQSTRN
                     ISSSSPPEKEQPPREEPRVPPLKIQLSKIGPPFIIKSQPVSKPESRASTSTSVSGGRNT
                     GARTLADIKARAQQARAQREAAAAAAVAAAASIVSGAMGSPGEGGKTRTLAHIKEQTKA
                     KLFAKHQARAHLFQTSKETRLPPPLSSKE"
     misc_feature    1579..1584
                     /label=SDS
     misc_feature    2085..2090
                     /label=SDS
     misc_feature    2166..2171
                     /label=SDS
     misc_feature    2213..2218
                     /label=SDS
     misc_feature    2617..2622
                     /label=SDS
     misc_feature    4121..4126
                     /label=SDS
     intron          4146..4186
                     /label=Splicing Donor
     misc_feature    4190..4223
                     /label=lox2272-JT1571
     repeat_region   4268..4408
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4268..4397
                     /label=AAV2 ITR
     rep_origin      4483..4938
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5220..5324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5325..6185
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6356..6944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctaaag acaagaggaa gaagaaggac cgcacctggg ccgaggctgc
      781 ccgcctggca ctagaaaaac accccaactc accaatgaca gcaaagcaga tattggaagt
      841 cattcagaaa gaagggttaa aagaaacaag tggaacctct ccattagcct gtctgaatgc
      901 aatgcttcac actaacactc gaatagggga tggaacattc ttcaaaatcc ctggaaagtc
      961 aggcctctat gctctcaaaa aagaggagtc gtcatgccca gcagatggca cgttggattt
     1021 agtctgtgaa tctgaattgg atggtacaga tatggccgag gcaaatgccc atggagaaga
     1081 aaatggagtt tgttcgaagc aggtaactga tgaagcatct tccactcgag attcaagcct
     1141 tactaacaca gcagtgcaaa gcaagttagt gtcttccttc cagcagcaca ccaaaaaggc
     1201 tcttaaacag gctttgaggc agcagcagaa aagaagaaat ggagtctcaa tgatggtaaa
     1261 caagactgtt cctcgtgttg ttttgacacc attaaaggtg tctgatgagc agtcggattc
     1321 gccttcagga tctgaatcta aaaatggtga agcagacagt tcagataaag aaatgaaaca
     1381 tgggcaaaaa tctcccactg gaaaacaaac aagtcagcac ttaaaacgat taaaaaagtc
     1441 tggtttaggg cacttgaaat ggaccaaagc tgaggacatt gacatagaaa ccccaggatc
     1501 tattcttgtc aacactaact tgagggcatt aataaataaa catacgtttg cttccttacc
     1561 tcagcatttt caacaatacc tcctgctttt gctcccagaa gtggataggc agatgggaag
     1621 tgatggaatt ttacgcctca gtacttcagc tctaaataat gaattctttg catatgcagc
     1681 acaagggtgg aaacagcgac tggcagaagg agagtttacc ccagaaatgc agttgcggat
     1741 aaggcaagaa attgagaagg aaaagaaaac agaaccttgg aaagaaaaat tctttgagag
     1801 gttttatgga gaaaagctgg gcatgtcaag agaggaatct gtgaagctca ctactggacc
     1861 aaacaacgct ggagctcaaa gtagttcttc atgtgggact tctggccttc cagtttctgc
     1921 acagacagcc ttggcagaac aacagccaaa aagcatgaaa agcccagctt ctccagagcc
     1981 tggtttctgt gctactcttt gccctatggt agaaattcca cctaaagata taatggcaga
     2041 attggagtca gaggatatct tgatccctga agaatctgta attcaggagg aaattgcaga
     2101 agaggtagag actagtatct gtgaatgcca ggatgaaaat cataagacaa tacctgaatt
     2161 ttctgaggag gctgaaagtc taaccaattc tcatgaagaa ccccaaatag cacctcctga
     2221 agataacttg gaatcctgtg ttatgatgaa tgatgtttta gaaactttgc ctcatattga
     2281 agttaagata gaagggaagt cagaatcacc ccaggaagaa atgacagttg ttatcgatca
     2341 gttagaagtc tgtgactctc ttattccttc cacttcatct atgactcatg tcagtgacac
     2401 agaacataag gagtcagaaa ctgcagtaga gaccagtacc cccaaaataa aaacagggtc
     2461 atcttctcta gaaggccagt ttccaaatga aggaattgct atagatatgg agctacagag
     2521 tgaccctgaa gaacagcttt cagaaaatgc ctgcatctct gaaacgtcct tttcttctga
     2581 gagcccagag ggagcctgta ccagcctgcc ttctccagga ggggaaacac agtccacatc
     2641 agaagaatca tgtactccag cctcccttga gacaacattt tgttctgagg tatctagcac
     2701 tgaaaataca gacaaataca accagagaaa ttccactgat gaaaactttc atgcatcttt
     2761 gatgtcagaa atatctccaa tatccacttc acctgaaata tcagaagcat ctcttatgtc
     2821 caacttacca ttaacatctg aagcatcacc agtatccaac ttacctttaa catcagaaac
     2881 ctcaccgatg tctgacttac ctttaacatc agaaacttct tcagtgtctt ccatgcttct
     2941 cacctctgag accacttttg tatccagttt gccacttcct tcagaaacat ctccaatttc
     3001 caactcttcc ataaatgaga gaatggcaca tcagcaaaga aagtcacctt ctgtatctga
     3061 agagccactc tccccgcaga aagatgagtc ttccgccact gccaaacctc tgggagagaa
     3121 ccttacctcc cagcagaaga atctgtctaa tactcccgaa cccatcataa tgagttcttc
     3181 ttccattgct cctgaagcat ttccgtctga agatttgcac aataagaccc tgagtcagca
     3241 aacctgtaaa tcacatgttg acactgagaa gccctaccct gcttcaattc cagaacttgc
     3301 ttctactgaa atgataaaag ttaaaaatca tagcgtcctg caaagaacag aaaaaaaagt
     3361 gttaccttca ccattggaat tatctgtctt ttctgaaggg acagataata agggaaatga
     3421 gcttccatct gctaaattac aggacaagca atatatctca tcagtggata aggctccatt
     3481 ttcagaaggc tctagaaata aaacacataa gcaagggagt acacagagtc ggttagaaac
     3541 ctcacatact tccaagtcat cagagccctc caagtcacct gatgggataa gaaatgaaag
     3601 tagagattca gagatatcaa agagaaaaac tgcagagcaa cacagctttg gaatctgtaa
     3661 ggaaaagaga gctaggatag aagatgatca gtcaacccgg aacatatcat ctagcagccc
     3721 acctgagaaa gaacagcctc ccagagagga accaagggtt ccccctctca agattcagct
     3781 ttccaaaatt gggccacctt ttataatcaa gagccaacca gtctccaaac ctgagtctcg
     3841 agcatccact agcacatctg tcagtggcgg gaggaacaca ggagccagga ccctcgcaga
     3901 tatcaaggcc cgggcccaac aagctcgggc ccagcgagag gctgctgcag ctgctgctgt
     3961 ggctgctgca gcgagcattg tctctggagc catgggaagt ccaggagagg gtggaaagac
     4021 gagaactctg gcacacatca aagagcagac aaaggctaag ctctttgcaa agcatcaagc
     4081 tcgagcccat ctcttccaga cctctaaaga gacccggttg cctcctccgc tcagctcaaa
     4141 ggaaggtaag tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta
     4201 taggatactt tatacgaagt tattaagtta tggagaccgg tgtggcacgt gcggaccgag
     4261 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4321 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4381 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4441 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     4501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     4561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4681 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4801 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4861 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4921 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4981 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5041 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5101 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5161 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5221 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5281 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5341 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5401 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5461 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5521 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5581 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5641 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5701 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5761 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5821 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5881 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5941 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6001 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6061 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6121 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6181 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6241 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6301 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6361 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6421 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6481 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6541 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6601 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6661 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6721 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6781 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6841 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6901 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6961 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//