| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-GLI3-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001597 |
| 产品用途: | 3' AAVLINK plasmid for GLI3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | GLI3 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-GLI3-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-GLI3-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-GLI3-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-GLI3-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-GLI3-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-GLI3-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-GLI3-FLAG 6189 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6189)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6189
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..2902
/codon_start=1
/label=GLI3
/translation="GTSSTSPAPSRTTAKCPTGPVTLTRPGCQTATLASSSMPSSSPAP
RAAKPTCPFSGTKSAPEAPTCPPPSSSVGRGPLCRRLAPLGSATAWSSTRRTP*GAGLL
GAIRPSGRTATPTVAQST*CSTTAPEVAPVETPSMNSPVRPRSMGTVSTGSQWPLVHST
VPVVPGFKPQS*RAPPCKGAGAS*ISACR*RQMSQLAAW*MACRTRTQWDRGTWLTSSS
ATACSTRGQAAPVSRCLGRLVLPHTSTSTKGQRAACQGLTAWAASRQAWQLSGATSHVP
ALGAAGARLCRGTALLCSQDSSVTQVRPAG*MVSRWR*KGSPIRCALICRITLVSSMTK
PWASVSKTRKLVHSLFQTPAACYRGPAPKTLSYFPQVLIR*QAQWTASTAMTWKGYRLT
SMPS*TMGTTPA*CRGP*AQVSFRTFPIAPPASPRLGRPSHSQRCP*APPTWLSGT*VL
C*PP*RKKANSLQLC"
misc_feature 1680..1685
/label=SDS
CDS 2909..2974
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2978..3409
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3122..3127
/label=SDS
repeat_region 3452..3592
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3452..3581
/label=AAV2 ITR
rep_origin 3667..4122
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4404..4508
/gene="bla"
/label=AmpR promoter
CDS 4509..5369
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5540..6128
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggtacgagc agcacttccc
1501 cagcgccctc ccggacgaca gcaaagtgcc ccacgggccc ggtgactttg acgcgcccgg
1561 gctgccagac agccacgctg gccagcagtt ccatgccctc gagcagccct gccccgaggg
1621 cagcaaaacc gacctgccca ttcagtggaa cgaagtcagc tccggaagcg ccgacctgtc
1681 ctcctccaag ctcaagtgtg ggccgcggcc cgctgtgccg cagactcgcg cctttgggtt
1741 ctgcaacggc atggtcgtcc acccgcagaa ccccttgagg agcgggcctg ctgggggcta
1801 tcagaccctc ggggagaaca gcaaccccta cggtggccca gagcacttga tgctccacaa
1861 cagccccgga agtggcacca gtggaaacgc cttccatgaa cagccctgta aggccccgca
1921 gtatgggaac tgtctcaaca ggcagccagt ggcccctggt gcactcgacg gtgcctgtgg
1981 tgccgggatt caagcctcaa agctgaagag cacccccatg caagggagcg ggggccagct
2041 gaatttcggc ctgccggtag cgccaaatga gtcagctggc agcatggtga atggcatgca
2101 gaaccaggac ccagtgggac aggggtacct ggctcaccag ctcctcggcg acagcatgca
2161 gcacccgggg gcaggccgcc ccggtcagca gatgcttggg cagattagtg ctacctcaca
2221 catcaacatc taccaagggc cagagagctg cctgccaggg gctcacggca tgggcagcca
2281 gccgtcaagc ttggcagttg tcaggggcta ccagccatgt gccagctttg ggggcagcag
2341 gcgccaggct atgccgaggg acagccttgc tctgcagtca ggacagctca gtgacacaag
2401 tcagacctgc agggtgaatg gtatcaagat ggagatgaaa gggcagcccc atccgctgtg
2461 ctctaatctg cagaattact ctggtcagtt ctatgaccaa accgtgggct tcagtcagca
2521 agacacgaaa gctggttcat tctctatttc agacgccagc tgcctgctac aggggaccag
2581 cgccaaaaac tctgagttac tttccccagg tgctaatcag gtgacaagca cagtggacag
2641 cctcgacagc catgacctgg aaggggtaca gattgacttc gatgccatca tagacgatgg
2701 ggaccactcc agcctgatgt cgggggccct gagcccaagt atcattcaga acctttccca
2761 tagctcctcc cgcctcacca cgcctcgggc gtccctccca ttcccagcgc tgtccatgag
2821 caccaccaac atggctatcg gggacatgag ttctttgctg acctccctag cggaagaaag
2881 caaattcctt gcagttatgc aatgtacaga ctacaaagac catgacggtg attataaaga
2941 tcatgacatc gactacaagg atgacgatga caagtaactc gagataatca acctctggat
3001 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
3061 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
3121 tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt
3181 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt
3241 gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga
3301 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
3361 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gatctggaga
3421 ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
3481 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
3541 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
3601 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
3661 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
3721 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
3781 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
3841 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
3901 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
3961 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
4021 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
4081 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
4141 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
4201 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
4261 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
4321 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
4381 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
4441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
4501 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
4561 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
4621 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
4681 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
4741 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
4801 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
4861 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
4921 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
4981 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
5041 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
5101 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
5161 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
5221 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
5281 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
5341 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
5401 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
5461 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
5521 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
5581 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
5641 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
5701 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
5761 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
5821 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
5881 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
5941 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
6001 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
6061 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
6121 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
6181 ctcacatgt
//
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