PX399

价格：1000元

联系方式：I47-825O-882O

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载体基本信息

出品公司:	Addgene
载体名称:	PX399 或 PX399 Mycoplasma gallisepticum str. F Cas9
质粒类型:	CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体
高拷贝/低拷贝:	高拷贝
插入位点:	限制性内切酶，多克隆位点
启动子:	CMV
载体大小:	11003 bp
5' 测序引物及序列:	CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列:	--
载体标签:	NLS (C ter)；HA、NLS (N ter)
载体抗性:	氨苄青霉素
筛选标记:	Neomycin
克隆菌株:	Stbl3
宿主细胞（系）:	昆虫细胞
备注:	--
产品目录号:	#68335
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	杆状病毒

载体图谱质粒图谱和多克隆位点信息

PX399载体图谱

载体简介

载体序列

LOCUS       PX399 Mycoplasma gallisepticum str. F Cas9	11003 bp 	DNA	SYN
DEFINITION  PX399 Mycoplasma gallisepticum str. F Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="PX399 Mycoplasma gallisepticum str. F Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5694
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
                     WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
                     FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
                     RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
                     NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
                     TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
                     DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
                     TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
                     RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
                     TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
                     AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
                     KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
                     KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
                     YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
                     DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
                     NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
                     VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
                     SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
                     EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
                     LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
                     NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
                     LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
                     DNFDILGLSKNRIDEILNSKLGLDKIVK*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
                     WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
                     FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
                     RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
                     NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
                     TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
                     DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
                     TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
                     RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
                     TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
                     AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
                     KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
                     KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
                     YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
                     DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
                     NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
                     VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
                     SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
                     EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
                     LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
                     NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
                     LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
                     DNFDILGLSKNRIDEILNSKLGLDKIVK*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
                     WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
                     FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
                     RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
                     NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
                     TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
                     DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
                     TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
                     RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
                     TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
                     AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
                     KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
                     KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
                     YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
                     DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
                     NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
                     VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
                     SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
                     EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
                     LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
                     NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
                     LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
                     DNFDILGLSKNRIDEILNSKLGLDKIVK*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
                     WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
                     FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
                     RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
                     NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
                     TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
                     DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
                     TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
                     RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
                     TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
                     AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
                     KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
                     KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
                     YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
                     DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
                     NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
                     VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
                     SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
                     EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
                     LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
                     NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
                     LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
                     DNFDILGLSKNRIDEILNSKLGLDKIVK*"
     CDS             complement(2752..3312)
                     /label="ORF frame 3"
                     /translation="MCLGQDLLIVFVSLFKQLEQPILLFLQDALEQFVRVGKFLQVGR
                     IDPIGISQVLAQVHKQGLQEIDLVQCCILVLQQEIQFQMLLQFVHKLSQGAQIVVLDF
                     AVGIQLQSLQFPLVVLLFQFLFILHILQCLFANAFGDCLFAQFLELPCVNWLLSVGFD
                     QQIQQLVQVGFLELLINEPASQVDAV*"
     CDS             complement(2805..3419)
                     /label="ORF frame 1"
                     /translation="MASKFQPLSLFFWLSSSLSRFVIRVIASSMALVDRLCVWARIFS
                     SSLSVCSNSLNSPSFCFCRMRSNSLLESENSFRLAESTLLEYLRCLPRYTNRDCRKLI
                     LSSVAYWSSSRKSSFRCCFSLCTNLARELRSLYWILPLVFSFSPFSFHLWSSFSSFSS
                     FSTSFSVSLPMLSAIVSSRSFLNCLASIGFSPLVLISRSSSLFR*"
     CDS             complement(4378..5073)
                     /label="ORF frame 3"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     misc_feature    complement(5727..5744)
                     /label="Sp6_primer"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     misc_feature    5749..5773
                     /label="attB2"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     terminator      5785..6251
                     /label="hGH_PA_terminator"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     terminator      6257..6292
                     /label="T7_terminator"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     rep_origin      6409..6715
                     /label="f1_origin"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     misc_feature    complement(6829..6849)
                     /label="pBABE_3_primer"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     misc_feature    complement(6835..7050)
                     /label="SV40_enhancer"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     promoter        6847..7115
                     /label="SV40_promoter"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     rep_origin      7014..7091
                     /label="SV40_origin"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     misc_feature    7076..7095
                     /label="SV40pro_F_primer"
                     /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                     GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                     HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                     VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                     QLVEQSLCLVAEN*"
     CDS             7230..8024
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            7233..8021
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7539..8075)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8201..8320
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8289..8308
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8511..8546
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8569..8586)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8576..8653
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8683..9300)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    9280..9313
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(10081..10941)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(10081..10941)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatgaac aatagcatca aatctaaacc tgaagtgacc
 1921 atcgggctgg acctgggagt gggaagcgtg gggtgggcaa tcgtggataa cgaaacaaac
 1981 atcattcacc atctgggctc caggctgttt tctcaggcca agactgctga ggatcggaga
 2041 tctttccgcg gggtgaggcg cctgatccga cggagaaaat acaagctgaa acgattcgtc
 2101 aatctgattt ggaagtacaa cagctatttc ggcttcaaga acaaagagga catcctgaac
 2161 aattatcagg agcagcagaa gctgcacaat accgtgctga acctgaaatc agaggcactg
 2221 aatgccaaga tcgatcctaa agcactgagc tggattctgc acgactacct gaagaacaga
 2281 ggccattttt atgaggacaa tagggatttc aacgtgtacc caacaaagga gctggccaag
 2341 tacttcgata agtacgggta ctacaaggga atcattgaca gcaaggagga caatgataac
 2401 aaactggagg aagagctgac aaagtacaaa ttctccaata agcactggct ggaagaggtg
 2461 aagaaagtcc tgtctaacca gactggcctg ccagaaaagt ttaaagaaga gtatgagtca
 2521 ctgttcagct acgtgagaaa ttattcagag ggcccaggga gcatcaactc tgtcagtccc
 2581 tacgggatct accatctgga cgaaaaagag ggaaaggtgg tccagaagta caacaacatc
 2641 tgggataaga caatcggaaa gtgcaacatc ttccctgacg agtatagagc tcccaagaac
 2701 agtcctatcg caatgatttt caatgaaatc aacgagctgt ccacaatcag gtcatacagc
 2761 atctacctga ctggctggtt cattaatcag gagttcaaga aagcctacct gaacaagctg
 2821 ctggatctgc tgatcaaaac caacggagag aagccaattg acgcaaggca gttcaagaaa
 2881 ctgcgcgaag agacaatcgc cgaaagcatt ggcaaagaga cactgaagga tgtggagaat
 2941 gaagagaaac tggaaaagga ggaccacaag tggaaactga agggactgaa gctgaatacc
 3001 aacggcaaaa tccagtacaa cgatctgagc tccctggcta agtttgtgca caaactgaag
 3061 cagcatctga aactggattt cctgctggag gaccagtatg caacactgga caagatcaat
 3121 ttcctgcagt ccctgtttgt gtacctgggc aagcacctga gatattccaa tagggtcgat
 3181 tctgccaacc tgaaggaatt ttccgactct aacaaactgt tcgagcgcat cctgcagaaa
 3241 cagaaggatg ggctgttcaa gctgtttgaa cagactgaca aagacgatga gaagatcctg
 3301 gcccagacac atagtctgtc aactaaggcc atgctgctgg ctattacccg gatgacaaat
 3361 ctggacaacg atgaggacaa ccagaaaaac aatgacaagg gctggaattt tgaggccatc
 3421 aaaaacttcg atcagaagtt tatcgacatc accaagaaaa acaacaacct gagcctgaaa
 3481 cagaataagc gctacctgga cgatcgattc atcaacgatg ctattctgtc ccctggggtg
 3541 aagcgaatcc tgcgggaggc aaccaaggtc tttaatgcca ttctgaaaca gttctctgaa
 3601 gagtacgacg tgacaaaggt ggtcatcgaa ctggctcgcg agctgagcga agagaaggaa
 3661 ctggagaaca caaagaacta caagaaactg atcaagaaaa acggcgacaa gattagtgag
 3721 ggcctgaaag cactggggat ctcagaagat gagatcaaag acattctgaa gagtcccact
 3781 aaatcataca agtttctgct gtggctgcag caggaccaca tcgatcctta tagcctgaag
 3841 gagatcgcct tcgacgatat ttttaccaaa acagaaaagt tcgagatcga ccatatcatt
 3901 ccctacagca tttccttcga cgattctagt tcaaacaagc tgctggtgct ggctgaaagt
 3961 aatcaggcaa agtcaaacca gactccttat gagttcatca gctccggaaa cgcaggcatt
 4021 aagtgggaag attacgaggc ctattgccgc aagttcaagg atggggactc tagtctgctg
 4081 gacagcaccc agcggtccaa gaaattcgcc aaaatgatga aaaccgatac ctcaagcaag
 4141 tacgacatcg gatttctggc tcgaaatctg aacgatactc ggtacgcaac cattgtgttc
 4201 cgggacgccc tggaggacta tgctaataac cacctggtcg aggacaaacc catgtttaag
 4261 gtggtctgta tcaatgggtc cgtgacctct ttcctgcgga agaactttga cgattcctct
 4321 tacgccaaga aagatagaga caagaatatc caccatgctg tggatgcaag tatcatctca
 4381 attttcagca acgagacaaa gactctgttc aaccagctga ctcagtttgc tgactataaa
 4441 ctgttcaaga acaccgatgg cagctggaag aaaatcgacc ctaagacagg ggtggtcact
 4501 gaagtgaccg acgagaattg gaagcagatt agggtgcgca accaggtgag cgaaatcgcc
 4561 aaagtcattg agaagtacat ccaggatagc aacatcgaaa gaaaggctag gtattcccgc
 4621 aaaatcgaga ataagactaa catttccctg tttaatgaca ccgtgtactc tgccaagaaa
 4681 gtcggctatg aggatcagat caaaagaaag aacctgaaaa ccctggacat tcacgaatct
 4741 gctaaagaga ataagaacag taaagtgaag cggcagtttg tctacagaaa gctggtgaat
 4801 gtcagcctgc tgaataacga taagctggca gacctgttcg ccgaaaaaga ggatatcctg
 4861 atgtataggg ccaatccatg ggtcatcaac ctggctgagc agattttcaa tgaatacact
 4921 gagaacaaga aaatcaagtc ccagaacgtg tttgaaaaat atatgctgga cctgaccaaa
 4981 gagttccccg agaagttcag cgagtttctg gtgaagtcca tgctgagaaa caagaccgcc
 5041 atcatctacg acgataagaa aaacattgtc catcgaatca aacggctgaa gatgctgagt
 5101 tcagaactga aagagaataa gctgtctaac gtgatcatta ggtctaagaa tcagagtggg
 5161 accaaactgt cataccagga tacaatcaac agcctggccc tgatgattat gcgcagcatc
 5221 gaccctactg ctaagaaaca gtatattcga gtgccactga ataccctgaa cctgcacctg
 5281 ggagatcatg actttgatct gcacaatatg gatgcttacc tgaagaaacc aaaattcgtg
 5341 aagtatctga aagcaaacga aatcggcgac gagtacaagc cctggagggt cctgacatct
 5401 ggcactctgc tgatccataa gaaggataag aaactgatgt acatcagctc cttccagaat
 5461 ctgaacgacg tgatcgaaat taagaatctg atcgaaaccg agtataaaga gaacgacgat
 5521 tctgatagta agaaaaagaa aaaggcaaac cgctttctga tgaccctgag cacaatcctg
 5581 aatgactaca ttctgctgga cgccaaggat aacttcgaca tcctggggct gtctaaaaat
 5641 cggatcgatg agattctgaa cagtaagctg ggactggaca agattgtgaa ataagaattc
 5701 ggtaccgagc tcaagcttgc ggccgcctat agtgtcacct aaatcgtaac ccagctttct
 5761 tgtacaaagt ggtcgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
 5821 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
 5881 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
 5941 aagttgggat gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
 6001 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
 6061 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
 6121 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
 6181 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
 6241 tccctgtcct tctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
 6301 ctagaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
 6361 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
 6421 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
 6481 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
 6541 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
 6601 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
 6661 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
 6721 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
 6781 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
 6841 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
 6901 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
 6961 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
 7021 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
 7081 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
 7141 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
 7201 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
 7261 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
 7321 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
 7381 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
 7441 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
 7501 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
 7561 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
 7621 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
 7681 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
 7741 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
 7801 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
 7861 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
 7921 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
 7981 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
 8041 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
 8101 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
 8161 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
 8221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
 8281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgtt catgaagccc
 8341 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
 8401 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
 8461 aatgagtatt tggtttagag tttggcaaca tatgcccata tgtaactagc ataacccctt
 8521 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag catgcggagg aaattctcct
 8581 tgaagtttcc ctggtgttca aagtaaagga gtttgcacca gacgcacctc tgttcactgg
 8641 tccggcgtat taaaacacga tacattgtta ttagtacatt tattaagcgc tagattctgt
 8701 gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc
 8761 tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt
 8821 aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg
 8881 tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc
 8941 caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa
 9001 aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt
 9061 gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt
 9121 gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc
 9181 cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc
 9241 gatcaaattt gtagttgagc tttttggaat tgcgatcgca taacttcgta tagcatacat
 9301 tatacgaagt tataagctcg gaacgctgcg ctcggtcgtt cggctgcggc gagcggtatc
 9361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
 9421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
 9481 tttccatagg ctccgccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
 9541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
 9601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
 9661 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
 9721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
 9781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
 9841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
 9901 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
 9961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
10021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
10081 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
10141 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
10201 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
10261 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
10321 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
10381 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
10441 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
10501 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
10561 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
10621 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
10681 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
10741 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
10801 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
10861 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
10921 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
10981 ttattgtctc atgtccgcgc gtt
//

pCold-GST	p3xFLAG-KV2.1
pMV261	pE
pcDNA3.1+P2A-eGFP	pNIT-3
PX391	pcDNA3.1+/N-GST(TEV)
pXIS11	pIAβ8
pGL4.77[hRlucP/Hygro]	IR2
pECFP-Nuc	pET302/NT-His
pHIC-PI	pVW5JI
pFastBac1	pCas-Guide-EF1a-GFP
pTCK303	pGL4.39[luc2P/ATF6 RE/Hygro]
pMKO.1-GFP	pFtsH_TA
pOS1	pLenti CMV/TO Neo empty (w215-1)
pAc5.1-V5-His A	pFastBac-GST-C
pHC79-2cos/tk	pcDNA6.2/V5-PL-DEST
pCDF1-MCS2-EF1-Puro	pDEST10
pFastBac Dual	pCMV-3Tag-3a
p53-Luc	pFB-hrGFP
pAX01-ComK	pBHR68
pFA1	pAc5.1a
pPZP122	pSW510
pLVX-Myc-MCS-Strep-IRES-Puro	pCambia1201
pKLAC2	pBApo-CMV-Pur
pCTAP-Shuttle-A	pYES2-EGFP
pYES2-kan	pQE-32
pCMV5	pcDNA4/V5-His B
pGL101	pcDNA3.1/His B
phCMV1	pFN31K Nluc-CMV-neo-Flexi
pcDNA4/HisMax A	pCAMBIA3200
pCR4-35	pLKO-DEST-EGFP
VaD1	pSinRep5
DR2	pWW60-1
pDP5rs	pME12::Tn5-259
pB-gyrase_TA	pSilencer5.1-H1 Retro
pJW342	pMYs-IRES-GFP
pET-28a-EGFP	pZeoSV2 (+)
pRS426	pGFP22
pMMB33	pEF4/His B
pCas	pEF1/His B
pTrcHis2 C	pG5luc
pTALETF_v2 (NG)	pPH3
pUCDM	pCB1535
pKO11	pTT5
pBad/Myc-His A	pGL4.25[luc2CP/minP]
pET-32 EK/LIC	pMIB/V5-His A
pMSCG19	pacAd5 9.2-100
pTYB2	pBT-5
pLVX-rHom-Sec1	pET-12c(+)
pSRE-Luc	pIJ702-87del(78-86, 88-132)
pIB/V5-His	pGL4.83[hRlucP/Puro]
pDR540	pSC101 and pKG2

xCas9(3.6)-BE3	pET15b_Bst-LF
pCAG Cas9-2A-Citrine	pRK5-p18-HA
pMDIAI	pRK GFP Paxillin
sfGFP-Cx43K258stop	pLenti PGK Puro DEST (w529-2)
pHIV-iRFP720-E2A-Luc	pMXs-hc-MYC
LentiGuide Cherry	pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
pRJPaph-bjGFP	pDA077
pAAV-EF1a-DIO-mCherry	hCas9_D10A
pBabe-puro-miR-10b sponge	pHIV-Luc-ZsGreen
PG13-luc (wt p53 binding sites)	pCMV-ABE7.9
pLentipuro3/TO/V5-GW/EGFP-Firefly Lucife	MSP2135
pCMVInt	DNMT3b KO Hyg
pCMV-SynA	pGL3-mArg1 promoter/enhancer -31/-3810
pFE-sfGFP	pHAGE2-TetOminiCMV-mCherry
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T	pTNT-B18R-6His
pEcgRNA	pPBbsr2-4031NES
pCas9	pHAGE2-TetOminiCMV-SKM
pGIPZ-PD-L1-EGFP	pSELECT-HA-mFOXO1
Stat3 Flag pRc/CMV	p415-GalL-Cas9-CYC1t
pcDNA3.1 myc-NL-GW	MSP680
pmGFP	pBS-hBAF60a
TrkC-GFP	pminiTol2
pCMVHA hEZH2	Flag-TRIM24
pcDNA3/Flag-METTL3	pHAGE NFkB-TA-LUC-UBC-GFP-W
pMaster1	pBECKP-km
pGL3 2 kb prom. CD274	pCMVR8.74
SIRT3 Flag	pBabe-hygro PyMT
pCAS9-TPC	pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-V
pmScarlet_Giantin_C1	Plasmid L5 attB::Pleft* mCherry
pTK299	pPH37
BECLIN-HA WT	pEGFP-NMHC II-C
pHAtC	pCMV-HsPeg10rc3
pc DNA FRT TO- APPSwed/Ind	pHyo094 (eMutaT7)
FUGW-PercevalHR	mTagBFP2-TOMM20-N-10
jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcode	pRN11
pCMV-2NLS-Cas9-6XMS2	pY001 (pFnCpf1_full)
pBFC1207	Tcf/Lef:H2B-GFP
pCI neo-hEST2	pET21a-BirA
pC0061 PsmCas13 (B05) His6-TwinStrep-SUM	pR26 GFP Dest
EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (F	pCRISPR-aBEST
pcDNA3.1-mCherry	CaV1.2
pGL4-ERSE2-luc2P-Hygro	pMSCV-FlagMLL-pl-ENL(5613)
mTert-pBabe-puro	pNLF1W
pSBbi-GN	pJQ200mp18
pBAD18-Cm	pAAV-EF1a-DIO-HTB
pUCmini-iCAP-AAV.CAP-B22	pGM1202
BCR/ABL P190 transgenic construct	pEJS654 All-in-One AAV-sgRNA-hNmeCas9
pYLCRISPR/Cas9pUbi-N	pSIN4-EF1a-LMO2-IRES-Puro
pLV-puro-N-3HA-SREBP1-C-Flag	pF151 pcDNA3.1(+)SOD1WT
cytoplasmic EKAR (Cerulean-Venus)	pNI3

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