| 质粒名称: | pCAG-luciferase |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 55764 |
| 存储实验室: | Snorri Thorgeirsson |
|
载体骨架基本信息 |
|
| 载体名称: | pGL4.17[luc2/Neo] |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5599 bp |
| 完整质粒大小: | 7323 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CMV enhancer/chicken beta actin promoter |
| 插入大小: | 1732 bp |
| 启动子 | CMV enhancer/chicken beta actin promoter |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SalI(未破坏) |
| 3' 克隆位点: | EcoRV(未破坏) |
| 5' 测序引物 | RV3 primer |
| 3' 测序引物 | pCAG-F |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG-luciferase was a gift from Snorri Thorgeirsson (爱迪基因 plasmid # 55764) |
| 参考文献部分: | Definition of ubiquitination modulator COP1 as a novel therapeutic target in human hepatocellular carcinoma. Lee YH, Andersen JB, Song HT, Judge AD, Seo D, Ishikawa T, Marquardt JU, Kitade M, Durkin ME, Raggi C, Woo HG, Conner EA, Avital I, Maclachlan I, Factor VM, Thorgeirsson SS. Cancer Res. 2010 Nov 1;70(21):8264-9. doi: 10.1158/0008-5472.CAN-10-0749. Epub 2010 Oct 19. 10.1158/0008-5472.CAN-10-0749 PubMed 20959491 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAG-luciferase 7323 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION luciferase reporter for in vivo bioluminescence imaging.
ACCESSION .
VERSION .
KEYWORDS pCAG-luciferase
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7323)
AUTHORS Lee YH, Andersen JB, Song HT, Judge AD, Seo D, Ishikawa T, Marquardt
JU, Kitade M, Durkin ME, Raggi C, Woo HG, Conner EA, Avital I,
Maclachlan I, Factor VM, Thorgeirsson SS
TITLE Definition of ubiquitination modulator COP1 as a novel therapeutic
target in human hepatocellular carcinoma.
JOURNAL Cancer Res. 2010 Nov 1;70(21):8264-9. doi:
10.1158/0008-5472.CAN-10-0749. Epub 2010 Oct 19.
PUBMED 20959491
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCAG-luciferase|
FEATURES Location/Qualifiers
source 1..7323
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 36..415
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 418..693
/label=chicken beta-actin promoter
intron 694..1702
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1626..1648
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1710..1729
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 1818..1827
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1824..3476
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 3520..3641
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3531..3550)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3585..3604
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
promoter 3835..4192
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3858..3878)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 4043..4178
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4105..4124
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4223..5017
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5042..5090
/note="synthetic polyadenylation signal"
primer_bind complement(5246..5263)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5417..6005)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5497..5516)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6205..7065)
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 7170..7218
/note="synthetic polyadenylation signal"
misc_feature 7232..7323
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 7272..7291
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgacat tgattattga ctagttatta
61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
241 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgggtcg
421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
541 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
721 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
841 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
901 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
961 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
1021 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
1081 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
1141 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
1201 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
1261 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
1321 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
1381 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
1441 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
1501 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
1561 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
1621 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
1681 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
1741 ttttggcaaa gaattcctcg aggatatcaa gatctggcct cggcggccaa gcttggcaat
1801 ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag ggcccagcgc
1861 cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct
1921 acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct
1981 acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga
2041 atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt
2101 tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg
2161 agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc
2221 tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg
2281 atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc
2341 cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg
2401 ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc
2461 gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc
2521 ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc
2581 tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat
2641 tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc acactattta
2701 gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg
2761 ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc
2821 taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc
2881 ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg
2941 tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc
3001 gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg
3061 acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct
3121 tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg
3181 aactggagag catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc
3241 ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca
3301 tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc
3361 gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc
3421 gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc gtgtaataat
3481 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
3541 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
3601 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
3661 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
3721 acaaatgtgg taaaatcgat aaggatccgt ttgcgtattg ggcgctcttc cgctgatctg
3781 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttagcta ccttctgagg
3841 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
3901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
3961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
4021 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
4081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
4141 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcga
4201 ttcttctgac actagcgcca ccatgatcga acaagacggc ctccatgctg gcagtcccgc
4261 agcttgggtc gaacgcttgt tcgggtacga ctgggcccag cagaccatcg gatgtagcga
4321 tgcggccgtg ttccgtctaa gcgctcaagg ccggcccgtg ctgttcgtga agaccgacct
4381 gagcggcgcc ctgaacgagc ttcaagacga ggctgcccgc ctgagctggc tggccaccac
4441 cggcgtaccc tgcgccgctg tgttggatgt tgtgaccgaa gccggccggg actggctgct
4501 gctgggcgag gtccctggcc aggatctgct gagcagccac cttgcccccg ctgagaaggt
4561 ttctatcatg gccgatgcaa tgcggcgcct gcacaccctg gaccccgcta cctgcccctt
4621 cgaccaccag gctaagcatc ggatcgagcg tgctcggacc cgcatggagg ccggcctggt
4681 ggaccaggac gacctggacg aggagcatca gggcctggcc cccgctgaac tgttcgcccg
4741 actgaaagcc cgcatgccgg acggtgagga cctggttgtc acacacggag atgcctgcct
4801 ccctaacatc atggtcgaga atggccgctt ctccggcttc atcgactgcg gtcgcctagg
4861 agttgccgac cgctaccagg acatcgccct ggccacccgc gacatcgctg aggagcttgg
4921 cggcgagtgg gccgaccgct tcttagtctt gtacggcatc gcagctcccg acagccagcg
4981 catcgccttc taccgcttgc tcgacgagtt cttttaatga tctagaaccg gtcatggccg
5041 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg ttcgaactag
5101 atgctgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
5161 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
5221 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
5281 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
5341 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
5401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
5461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
5521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
5581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
5641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
5701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
5761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
5821 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
5881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
5941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
6001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
6061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
6121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc
6181 gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc
6241 gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat
6301 tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc
6361 ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc
6421 tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag
6481 ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg
6541 ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg
6601 atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag
6661 taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt
6721 catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga
6781 gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc
6841 acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc
6901 aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc
6961 ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc
7021 cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca
7081 atattattga agcatttatc agggttacta gtacgtctct caaggataag taagtaatat
7141 taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt
7201 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga
7261 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc
7321 tct
//
